Lrit1 (NM_146245) Mouse Tagged ORF Clone

CAT#: MR209465

Lrit1 (Myc-DDK-tagged) - Mouse leucine-rich repeat, immunoglobulin-like and transmembrane domains 1 (Lrit1)

Product Images

Circular map for MR209465

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrit1
• Synonyms: BC032270; Lrrc21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209465 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGTGGCCCTGGGCATGCTCTGGCTCCTCGCACTTGGGGGACCTCACCAGGCCTGGGGCTTCTGTC
  CTTCTGAATGCAGCTGCAGTCTGCGCATCCTGAGTGACGGCAGCAAGGCCAGGACAGTGGTGTGCAGCGA
  CCCTGACTTGACTCTGCCTCCAGCTTCGATTCCTCCAGACACCTGCAAGCTGCGCTTAGAGAGAACCGCC
  ATTCGCAGGGTGCCGGGAGAGACCTTCAGGCCTCTCAGCCGCCTGGAGCAGCTGTGGCTACCTTACAATG
  CTCTCAGTGAGCTTAGTGCCCTCATGCTCAGGGGCCTGAGACGCCTACGAGAGCTGCGGCTGCCTGGGAA
  CCGCCTGGTCACGTTCCCCTGGGCTGCGCTGAGGGACACTCCGCAGCTGCAGCTGCTGGACCTGCAGGCC
  AATCGCCTCTCGACCTTGCCACCCGAGGCTGCACACTTCCTGGAGAACCTTACTTTCCTGGACCTGTCCA
  ATAACCAGCTGATGAGGCTTCCTGAGGAGCTACTGGACGTGTGGGCTCACCTGAAGACCGGGCCCTTCCT
  TTCCGGCCATCATGCCAGGCTAATCTTAGGGCTTCAGGACAACCCCTGGGTGTGTGACTGTCGGCTCTAT
  GACCTGGTTCATCTTCTAGATGGCTGGGTTTCTTCAAACCTGATCTTCATCGAGGCTAGACTGAGATGTG
  CCAGTCCACGCAGCCTGGCTGGAGTGGCCTTCAGCCAGCTGGAGCTAAGAAAGTGTCAGAGCCCAGAGCT
  CCGTCCAGGGGTGACCAGCATCATATCCCCTTTGGGTAGCACAGTATTGCTACGTTGTGGAGCAACTGGG
  ATCCCAGGACCTGAGATGAGCTGGAGAAGGGCCAATGGACGACCACTCAATGGCACAGTACACCAGGAAG
  TCTCCAGTGACGGCTCAAGTTGGACTTTGCTAGATTTGCCTGTTGTGTCTCTCTTTGACTCTGGGGACTA
  CATCTGCCAAGCCAAGAACTTCCTGGGAGCTTCTGAAACCCTGATCTCCTTGATTGTCACTGAGCCACAG
  ACTTCTACCGGATACAGTGGGATTCCAGGTGTCCTGTGGGCAAGAACAGGGGAGGGGGCAGAAGCTGCTG
  CCTACAACAACAAGCTGGTGGCCAGGCATGTTCCTCATATGCCCGAGCATGTAGCCCTGGCTACCAAGCC
  CTCAATGCCCAGCATAAAGGAGGAGCTGGCTCTCCAGAACTTTCAGATGGATGTCCCAGGAGAGTTCTCC
  AGAGAGCCATCAGAACACCAGGAGGCACAGATGGTCAGGTCTCTCAAGGTAGTAGGAGATACTTACCACA
  GTGTGTCTTTGGTGTGGAAGGCCCCTCAAGCTGGGAACACAACCGCCTTTAGTGTCCTTTATGCAGTCTT
  TGGGCATCGAGACATGAGAAGGATGACTGTGGAGCCTGGGAAGACTAGTGTCACTATCGAGGGACTTGCT
  CCAAAGACCAAGTATGTGGCATGTGTCTGTGTGCGGGGCTTGGTGCCTACGAAGGAGCAATGTGTCATCT
  TCTCTACTGATGAGGTAGTGGATGCAGAGGGCACCCAGCGACTCATCAACATGGTGGTGATCAGCGTGGC
  CGCCATCATCGCGCTGCCTCCCACCCTGCTGGTTTGCTGTGGGGCTCTCCGAAGACGCTGCCACAAGTGC
  CGCACTGGGGGTTCTGCAGAGGCCTCTGGGGCCTATGTTAATTTGGAAAGACTGGGCCATAGTGAGGACA
  GCTCAGAAGTTCTGTCCAGGAGCAGCCTCAGTGAGGGAGATAGGCTTCTCTCAGCCCGTTCCAGCCTGGA
  CTCCCAGGTCTTGGGTGTCAGGGGCGGCAGACGCATCAATGAGTACTTCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209465 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWVALGMLWLLALGGPHQAWGFCPSECSCSLRILSDGSKARTVVCSDPDLTLPPASIPPDTCKLRLERTA
  IRRVPGETFRPLSRLEQLWLPYNALSELSALMLRGLRRLRELRLPGNRLVTFPWAALRDTPQLQLLDLQA
  NRLSTLPPEAAHFLENLTFLDLSNNQLMRLPEELLDVWAHLKTGPFLSGHHARLILGLQDNPWVCDCRLY
  DLVHLLDGWVSSNLIFIEARLRCASPRSLAGVAFSQLELRKCQSPELRPGVTSIISPLGSTVLLRCGATG
  IPGPEMSWRRANGRPLNGTVHQEVSSDGSSWTLLDLPVVSLFDSGDYICQAKNFLGASETLISLIVTEPQ
  TSTGYSGIPGVLWARTGEGAEAAAYNNKLVARHVPHMPEHVALATKPSMPSIKEELALQNFQMDVPGEFS
  REPSEHQEAQMVRSLKVVGDTYHSVSLVWKAPQAGNTTAFSVLYAVFGHRDMRRMTVEPGKTSVTIEGLA
  PKTKYVACVCVRGLVPTKEQCVIFSTDEVVDAEGTQRLINMVVISVAAIIALPPTLLVCCGALRRRCHKC
  RTGGSAEASGAYVNLERLGHSEDSSEVLSRSSLSEGDRLLSARSSLDSQVLGVRGGRRINEYFC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146245
• ORF Size: 1875 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146245.2
• RefSeq Size: 4337 bp
• RefSeq ORF: 1875 bp
• Locus ID: 239037
• UniProt ID: Q8K099
• Cytogenetics: 14 B
• MW: 68.5 kDa
• Gene Summary: Possible role in phototransduction.[UniProtKB/Swiss-Prot Function]