Ppm1d (BC023492) Mouse Tagged ORF Clone

CAT#: MR209279

Ppm1d (Myc-DDK-tagged) - Mouse protein phosphatase 1D magnesium-dependent, delta isoform (cDNA clone MGC:32306 IMAGE:5026376)

Product Images

Circular map for MR209279

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppm1d
• Synonyms: AV338790; Wip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209279 representing BC023492
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCGGACCAGGGCGGGAGGAAGTACATGGAGGACG
  TAACTCAGATCGTGGTGGAGCCCGAGCCGGCTGCGGAGGACAAGCCGGCGCCGGTACCGCGGCGGGCGCT
  CGGGTTGCCGGCGACCCCCACTCTCGCCGGCGTCGGGCCATCGGAAAAAGGCCCGGCGGCGGCCCGCGAC
  CCTGCCCCGGACGCCGCGGCCTCGCTACCCGCTGGCCGCTGCTGTCGCCGCCGCTCTTCGGTGGCCTTCT
  TTGCAGTGTGCGACGGGCACGGCGGTCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTGTGGGGTTTCAT
  CAAGAAGCAGAAAGGCTTCACCTCGTCCGAGCCCGCCAAGGTGTGCGCTGCCATCCGCAAAGGTTTCCTC
  GCCTGTCACCTCGCCATGTGGAAGAAACTGGAATGGCCAAAGACTATGACAGGTCTTCCCAGCACGTCCG
  GGACAACTGCCAGTGTGGTTATAATACGGGGCATGAAGATGTATGTAGCGCATGTAGGTGACTCTGGGGT
  GGTCCTTGGAATTCAGGATGACCCAAAGGATGATTTTGTGAGAGCTGTGGAGGTGACACAAGATCACAAG
  CCAGAACTTCCCAAGGAAAGAGAAAGAATCGAAGGACTTGGCGGCAGTGTGATGAACAAGTCTGGAGTGA
  ACCGAGTAGTTTGGAAAAGGCCCCGGCTCACTCACAGTGGACCTGTCAGAAGGAGCACAGTCATTGACCA
  GATTCCTTTTCTGGCTGTAGCAAGAGCGCTTGGTGACTTGTGGAGCTATGATTTCTTCAGTGGTAAGTTT
  GTGGTGTCACCTGAACCAGACACCAGTGTCCACACTCTTGACCCCCGGAAGCACAAGTATATTATCCTGG
  GAAGTGATGGACTTTGGAATATGGTTCCACCTCAAGATGCCATCTCCATGTGCCAAGACCAAGAGGAGAA
  AAAATACTTGATGGGTGAGCAAGGACAGTCCTGTGCCAAAATGCTTGTGAATCGAGCACTTGGCCGCTGG
  AGGCAGCGTATGCTTCGGGCAGATAACACAAGTGCCATCGTAATCTGCATCTCTCCAGAAGTAGACAACC
  AAGGGAACTTCACCAATGAAGATGAGCTCTTTCTGAACCTGACTGATAGCCCTACTTACAACAGCCAGGA
  GACCTGTGTGATGACTTCTTCTCCAAGTTCTACACCACCAATCAAGTCACCGGAAGAAGATGCATGGCCA
  AGGCTGAGCTCTAAGGACCATATACCTGCCCTTGTTCGCAGTAATGCCTTCTCAGAGAAGTTTTTAGAGG
  TCCCAGCTGAGATAGCTAGAGGGAATATCCAGACTGTAGTGATGACCTCAAAAGACTCAGAGACACTTGA
  AGAAAATTGCCCCAAAGCCCTGACTTTAAGGATTCATGATTCTTTGAATAATACTCTGTCAGTTGGCCTC
  ATTCCAACCAATTCAACAAATACTATCATGGACCAAAAAAACTTAAAGATGTCAACTCCAGGTCAAATGA
  AAGCTCAAGAAGTTGAAAGAACCCCTCCAGCCAATTTTAAAAGGACATTAGAAGAATCCAACTCTGGCCC
  CCTTATGAAGAAGCACCGACGAAATGGCTTAAGTCGAAGTAGCGGGGCCCAGGCTTCCAGTCTCCCTACA
  GCATCCCAGCGCAGGCACTCTGTCAAACTGACCCTGAGGCGCAGACTCAGGGGCCAGAGGAAGATGGGAA
  ATCCTCTTCTCCACCAGCACCGGAAAACAGTGTGTGTGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209279 representing BC023492
  Red=Cloning site Green=Tags(s)
  
  MAGLYSLGVSVFSDQGGRKYMEDVTQIVVEPEPAAEDKPAPVPRRALGLPATPTLAGVGPSEKGPAAARD
  PAPDAAASLPAGRCCRRRSSVAFFAVCDGHGGREAAQFAREHLWGFIKKQKGFTSSEPAKVCAAIRKGFL
  ACHLAMWKKLEWPKTMTGLPSTSGTTASVVIIRGMKMYVAHVGDSGVVLGIQDDPKDDFVRAVEVTQDHK
  PELPKERERIEGLGGSVMNKSGVNRVVWKRPRLTHSGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGKF
  VVSPEPDTSVHTLDPRKHKYIILGSDGLWNMVPPQDAISMCQDQEEKKYLMGEQGQSCAKMLVNRALGRW
  RQRMLRADNTSAIVICISPEVDNQGNFTNEDELFLNLTDSPTYNSQETCVMTSSPSSTPPIKSPEEDAWP
  RLSSKDHIPALVRSNAFSEKFLEVPAEIARGNIQTVVMTSKDSETLEENCPKALTLRIHDSLNNTLSVGL
  IPTNSTNTIMDQKNLKMSTPGQMKAQEVERTPPANFKRTLEESNSGPLMKKHRRNGLSRSSGAQASSLPT
  ASQRRHSVKLTLRRRLRGQRKMGNPLLHQHRKTVCVC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023492
• ORF Size: 1791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023492.1
• RefSeq Size: 2023 bp
• RefSeq ORF: 1793 bp
• Locus ID: 53892
• Cytogenetics: 11 51.34 cM
• MW: 74.1 kDa
• Gene Summary: Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (By similarity). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (PubMed:24135283).[UniProtKB/Swiss-Prot Function]