Chuk (BC018243) Mouse Tagged ORF Clone

CAT#: MR209133

Chuk (Myc-DDK-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275)

Product Images

Circular map for MR209133

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chuk
• Synonyms: IKK1, Chuk1, IKK-1, IKK[a], IKK-alpha, IKKalpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209133 representing BC018243
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGCCCCTGGGAGATGCGGGAACGGCTTGGCACCG
  GCGGTTTCGGGAACGTCAGTCTGTACCAGCACCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCG
  TTTAGAGCTAAGTTCCAAAAACAGAGAGCGATGGTGCCATGAAATCCAGATCATGAAAAAGTTGGACCAT
  GCGAATGTTGTAAAGGCCTGTGATGTCCCTGAGGAATTGAACTTTTTAATTAACGATGTGCCTCTTCTGG
  CAATGGAGTACTGTTCTGGAGGGGACCTCCGGAAGCTACTCAACAAACCAGAAAATTGTTGTGGACTTAA
  AGAAAGCCAGATACTTTCTTTACTGAGTGACATAGGATCTGGGATCCGATATCTGCATGAAAACAAAATT
  ATACATCGAGATCTAAAACCTGAAAATATAGTTCTTCAAGATGTTGGTGGGAAGACAATACATAAAATAA
  TTGATTTGGGTTATGCCAAAGATGTTGATCAAGGAAGTCTCTGTACATCTTTTGTGGGAACATTGCAGTA
  TTTGGCCCCAGAGCTCTTTGAAAATAAGCCGTACACAGCCACTGTGGATTATTGGAGCTTTGGGACCATG
  GTGTTTGAATGTATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGCATGAGAAGA
  TTAAGAAGAAAGATCCAAAGTGTATATTTGCATGTGAAGAGATGACTGGAGAAGTTCGGTTTAGTAGCCA
  TTTACCTCAGCCAAACAGCCTTTGTAGTTTAATAGTAGAGCCAATGGAAAGCTGGCTCCAATTGATGCTG
  AATTGGGACCCACAGCAGAGAGGGGGACCTATTGATCTTACTTTGAAGCAGCCAAGATGTTTTGCATTAA
  TGGATCACATTCTCAATTTAAAGATAGTGCACATCCTAAATATGACTTCTGCAAAAATCATTTCTTTTCT
  GTTACCATGTGATGAAAGTCTTCATTCACTACAGTCTCGAATTGAGCGTGAAACAGGAATAAATACAGGT
  TCTCAGGAGCTTCTGTCAGAGACAGGGATTTCTCTGGATCCTCGGAAACCAGCCTCTCAGTGTGTTCTAG
  ATGGAGTTAGAGGCTGTGATAGCTACATGGTTTATTTGTTTGATAAAAGTAAGACTGTATATGAAGGACC
  ATTTGCATCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAAGACAGCAAAATACAACTGCCAATT
  ATACAGCTGCGGAAAGTATGGGCTGAAGCAGTGCACTACGTATCTGGGCTAAAGGAAGACTACAGCAGGC
  TCTTCCAGGGACAAAGAGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTGACAAAAATGAAGAA
  TACTTTGATCTCAGCATCACAGCAACTCAAAGCTAAATTGGAGTTTTTTCGAAAAAGCATTCAGCTTGAC
  TTGGAGAGATATAGTGAGCAGATGACTTATGGGATATCTTCAGAAAAAATGTTAAAAGCATGGAAAGAAA
  TGGAAGAAAAGGCCATTCACTATTCTGAGGTTGGTGTCATTGGTTATCTTGAGGATCAAATTATGTCTTT
  GCACACTGAAATCATGGAGCTGCAGAAGAGCCCCTATGGACGACGCCAGGGAGACTTGATGGAGTCTCTG
  GAGCAGCGTGCCATTGATCTCTATAAGCAGCTAAAGCACAGACCTCCTGGTAAGACACTTCAGTCACAGT
  AT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209133 representing BC018243
  Red=Cloning site Green=Tags(s)
  
  MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSSKNRERWCHEIQIMKKLDH
  ANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKI
  IHRDLKPENIVLQDVGGKTIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTM
  VFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNSLCSLIVEPMESWLQLML
  NWDPQQRGGPIDLTLKQPRCFALMDHILNLKIVHILNMTSAKIISFLLPCDESLHSLQSRIERETGINTG
  SQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPI
  IQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLD
  LERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESL
  EQRAIDLYKQLKHRPPGKTLQSQY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC018243
• ORF Size: 1752 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018243, AAH18243
• RefSeq Size: 2056 bp
• RefSeq ORF: 1754 bp
• Locus ID: 12675
• Cytogenetics: 19 36.71 cM
• MW: 75.4 kDa