Optn (NM_181848) Mouse Tagged ORF Clone

CAT#: MR209132

Optn (Myc-DDK-tagged) - Mouse optineurin (Optn)

Product Images

Circular map for MR209132

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Optn
• Synonyms: 4930441O07Rik; FIP2; HYPL; NRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209132 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCATCAACCTCTGAGCTGCCTGACTGAGAAGGGGGACAGCCCTTGTGAGACCCCAGGAAATGGAC
  CCTCCAATATGGTTCACCCCAGCCTGGACACATTCACCCCTGAGGAGCTGCTGCAGCAAATGAAGGAACT
  CCTGGTTGAGAACCACCAGCTGAAAGAAGCCATGAAGCTAAATAATCAAGCTATGAAAGGGCGATTTGAG
  GAGCTGTCCGCCTGGACAGAGAAGCAGAAGGAAGAGCGCCTGTTGTTTGAGATGCAAAGCAAAGAGGTTA
  AGGAGCGCCTTAAGGCCCTGACTCATGAAAATGAGAGGCTGAAGGAAGAGCTTGGAAAATTCAAAGAGAA
  ATCAGAAAAGCCATTGGAAGACCTCACAGGTGGCTACAGGTATCCCAGAGCCTTGGAGGAGGAAGTGGAG
  AAGCTGAAGACCCAGGTGGAGCAGGAAGTGGAGCATCTGAAGATCCAGGTGATGCGCCTTCGGGCTGAAA
  AGGCAGACCTGCTGGGCATCGTCTCAGAACTGCAGCTCAAACTCAACTCCGGCGGCTCCTCGGAAGACTC
  CTTCGTTGAGATCAGGATGACCGAAGGAGAGACTGAAGGGGCAATGAAGGAGATGAAGAACTGCCCTACA
  CCCACAAGAACAGACCCCATCAGCTTGAGCAACTGTACAGAGGATGCCAGGAGTTGTGCGGAGTTTGAAG
  AACTGACTGTGAGCCAGCTTCTGCTTTGCCTAAGGGAAGGAAACCAAAAGGTGGAGAGACTTGAAGTCGC
  CCTCAGAGAAGCCAAAGAAAGAATTTCAGATTTTGAAAAGAAAGCAAATGGCCATTCTTCTACTGAGAAG
  CAGACAGCGAGGAGAGCAGACAGAGAGAAGGAGGACAAAGGCCAAGAGAGTGTTGGAAGCGAAGTGGAAA
  CACTGAGCATTCAAGTGACCTCTCTGTTTAAGGAGCTTCAAGAGGCACACACAAAACTCAGTGAGGCTGA
  GCTGATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCTCTGGAGAGGAAGAACTCTGCAACACCATCAGAG
  CTGAATGAAAAGCAAGAGCTCGTTTACAGTAACAAGAAGTTAGAGCTGCAGGTGGAGAGCATGCGCTCCG
  AAATCAAGATGGAGCAGGCCAAGACAGAGGAGGAGAAGTCCAGGTTAGCCACTCTGCAGGCAACTCACAA
  CAAGCTCCTTCAAGAACATAATAAGGCACTGAAAACAATTGAAGAACTAACCAAGCAACAGGCAGAAAAG
  GTGGACAAGATGTTGCTGCAGGAGCTCAGCGAGAAGCTGGAGCTGGCAGAGCAGGCTCTGGCATCCAAAC
  AGCTCCAGATGGATGAGATGAAGCAGACGCTCGCTAAGCAGGAGGAAGACCTGGAGACCATGGCCGTCCT
  CAGGGCTCAGATGGAGGTGTACTGCTCAGATTTTCACGCTGAGAGAGCAGCAAGAGAGAAGATTCATGAA
  GAAAAGGAGCAGCTGGCCTTGCAGCTCGCGATTTTGCTGAAAGAGAACAATGACATTGAAGAGGGAGGCA
  GTAGACAGTCCCTGATGGAAATGCAGTGCCGACACGGGGCAAGAACCAGTGACTCTGACCAGCAGACTTA
  CCTGTTTCAAAGAGGAGCCGAGGACAGGAGCTGGCAGCACGGGCAGCAGCCTCGCAGTATTCCGATTCAC
  TCCTGCCCCAAGTGCGGAGAGGTCCTGCCGGACATCGACACGCTTCAGATCCATGTGATGGACTGCATCA
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209132 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHQPLSCLTEKGDSPCETPGNGPSNMVHPSLDTFTPEELLQQMKELLVENHQLKEAMKLNNQAMKGRFE
  ELSAWTEKQKEERLLFEMQSKEVKERLKALTHENERLKEELGKFKEKSEKPLEDLTGGYRYPRALEEEVE
  KLKTQVEQEVEHLKIQVMRLRAEKADLLGIVSELQLKLNSGGSSEDSFVEIRMTEGETEGAMKEMKNCPT
  PTRTDPISLSNCTEDARSCAEFEELTVSQLLLCLREGNQKVERLEVALREAKERISDFEKKANGHSSTEK
  QTARRADREKEDKGQESVGSEVETLSIQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSATPSE
  LNEKQELVYSNKKLELQVESMRSEIKMEQAKTEEEKSRLATLQATHNKLLQEHNKALKTIEELTKQQAEK
  VDKMLLQELSEKLELAEQALASKQLQMDEMKQTLAKQEEDLETMAVLRAQMEVYCSDFHAERAAREKIHE
  EKEQLALQLAILLKENNDIEEGGSRQSLMEMQCRHGARTSDSDQQTYLFQRGAEDRSWQHGQQPRSIPIH
  SCPKCGEVLPDIDTLQIHVMDCII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181848
• ORF Size: 1755 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181848.3
• RefSeq Size: 2413 bp
• RefSeq ORF: 1755 bp
• Locus ID: 71648
• UniProt ID: Q8K3K8
• Cytogenetics: 2 3.15 cM
• MW: 67 kDa
• Gene Summary: Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Plays a role in the activation of innate immune response during viral infection. Mechanistically, recruits TBK1 at the Golgi apparatus, promoting its trans-phosphorylation after RLR or TLR3 stimulation. In turn, activated TBK1 phosphorylates its downstream partner IRF3 to produce IFN-beta. Plays a neuroprotective role in the eye and optic nerve. May act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). Mediates the interaction of Rab8 with the probable GTPase-activating protein TBC1D17 during Rab8-mediated endocytic trafficking, such as of transferrin receptor (TFRC/TfR); regulates Rab8 recruitnment to tubules emanating from the endocytic recycling compartment. Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family; targets ubiquitin-coated bacteria (xenophagy), such as cytoplasmic Salmonella enterica, and appears to function in the same pathway as SQSTM1 and CALCOCO2/NDP52. May constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death.[UniProtKB/Swiss-Prot Function]