Rbm28 (BC018373) Mouse Tagged ORF Clone

CAT#: MR209032

Rbm28 (Myc-DDK-tagged) - Mouse RNA binding motif protein 28 (cDNA clone MGC:12075 IMAGE:3708613)

Product Images

Circular map for MR209032

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rbm28
• Synonyms: MGC12075, MGC38664
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209032 representing BC018373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGATAAAAGGACGGACTGTGGCTGTGGATTGGGCTGTGGCAAAGGACAAATACAAAGATGCCC
  AGCATGCTTCTGCCCCAGGTGTAAAGAAGAGCAGTGACCGGAAGCCTAAGGAATCAGGTAAGAAGAACTG
  CAGGGTGGAAGAACAAGTAGAAGATAGTGATGATGAAGAAGATGATGATAGCCATGATGACGAAGAAGAA
  AGAGAATCAACAATAGCTTCTCCTGTGAGTGTGCACAAGAGAGCAGTCAAGAGAGCAGCTCCCGAGGAAA
  GCATCGAGGAAGACGACTCCTACGAGGACAGTGACCTGGAGGAAGGAGGCAGCTCCTATGATGAGGGGAC
  AGTGGACAGTGAGAGCAGCGCTGAAGATCAAGAGGATGAAGATGTTCCAGTCTCAGAGAAAAAGAAGAGG
  AAGTTACCCTCGGATGTGACGGAAGGGAAAACTGTCTTCATCAGAAATCTTTCATTTGACTCAGAAGAAG
  AGGCACTTGGCGAGGTCCTCCAACAGTTTGGTGATCTTAAGTATGTCCGAGTTGTCTTGCATCCAGACAC
  AGAGCATTCTAAAGGTTGTGCATTCGCACAATTCATGACTCAGGAAGCAGCTCAGAAATGTCTTGCAGCG
  GCCTCTCTAGAGGCTGAGGGTGGTGGGCTTAAACTGGACGGCCGACAGCTCAAGGTCGACTTAGCTGTGA
  CCCGGGATGAGGCAGCAAAGCTTCAGACAAAGAAGGTGAAGAAGCCAACTGGAACCCGGAACCTCTATCT
  GGCCAGAGAAGGCTTGATCCGCGCTGGGACGAAGGCTGCTGAGGGTGTGAGTGCCGCAGATATGGCCAAA
  CGCGAGCGGTTTGAGCTACTGAAACATCAGAAACTCAAGAACCAGAATATCTTTGTCTCGCAGACCAGGC
  TTTGCCTGCACAATCTCCCAAAGGCTGTGGACGATAAACAGCTAAGGAAGCTGCTGCTGGAGGCCACTCG
  AGGAGAGAAGGGGGTTCGAATTAAGGAGTGTAGAGTGATGCGGGACCTTAAAGGAGTTCATGGGAAAATG
  AAGGGTCAGTCCCTGGGCTATGCCTTTGCAGAGTTCCAAAAGCATGAGCATGCCCTCAGAGCCCTCCGCC
  ACTTCAACAACAACCCAGAGATCTTTGGGTCCCAAAAGAGACCCATAGTGGAGTTTTCTTTAGAAGACCG
  AAGGAAACTTAAAGTGAAGGAGCTGAGGATCCAACGCAGCCTGCAAAAAATGGAATCCAAGCCTGTGACC
  AGTAAGCCCCAAAAGGAGCAAAAAGAAGTTGGAAAAGACAAGCAACAGAAAGCAGTTCAGAGCGCCACAC
  AGGATCAGAGTAAGGCGCCTGGAGAGCAGAAGGGGAAAGCCGGCTCCACCTCGTGGACAGGCTTCCAGAC
  CAAGGCTGAAGTGGAGCAGGTGGAGCTGCCTGACGGGAAGAAGAGAAGAAAGGTCCTGGCACTCCCTTCA
  CACCGAGGCCCCAAAATTAGGCTGCGGGACAAAGGCAAAGTGAAGTCCCTGCCTTCTAGGAAGCCAAAGC
  CCCAGATGGGCCAGCGGAAGCAAAAGAAGCAGCAGTTAGCGTCTTCCGTGCAGGCGCCTAAAAGAAAGGC
  TAAGGAGAATAAGGCAGAAGCCCGCTTCAACCAGCTGGTTGAACAATATAAGCAGAAGTTGCTGGGCCCT
  TCTAAAGGAGCGCCCCTCATGAAGAGAAGCAAATGGTTTGACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209032 representing BC018373
  Red=Cloning site Green=Tags(s)
  
  MKEIKGRTVAVDWAVAKDKYKDAQHASAPGVKKSSDRKPKESGKKNCRVEEQVEDSDDEEDDDSHDDEEE
  RESTIASPVSVHKRAVKRAAPEESIEEDDSYEDSDLEEGGSSYDEGTVDSESSAEDQEDEDVPVSEKKKR
  KLPSDVTEGKTVFIRNLSFDSEEEALGEVLQQFGDLKYVRVVLHPDTEHSKGCAFAQFMTQEAAQKCLAA
  ASLEAEGGGLKLDGRQLKVDLAVTRDEAAKLQTKKVKKPTGTRNLYLAREGLIRAGTKAAEGVSAADMAK
  RERFELLKHQKLKNQNIFVSQTRLCLHNLPKAVDDKQLRKLLLEATRGEKGVRIKECRVMRDLKGVHGKM
  KGQSLGYAFAEFQKHEHALRALRHFNNNPEIFGSQKRPIVEFSLEDRRKLKVKELRIQRSLQKMESKPVT
  SKPQKEQKEVGKDKQQKAVQSATQDQSKAPGEQKGKAGSTSWTGFQTKAEVEQVELPDGKKRRKVLALPS
  HRGPKIRLRDKGKVKSLPSRKPKPQMGQRKQKKQQLASSVQAPKRKAKENKAEARFNQLVEQYKQKLLGP
  SKGAPLMKRSKWFDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC018373
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC018373.1
• RefSeq Size: 2117 bp
• RefSeq ORF: 1727 bp
• Locus ID: 68272
• Cytogenetics: 6 A3.3
• MW: 77.6 kDa
• Gene Summary: Nucleolar component of the spliceosomal ribonucleoprotein complexes.[UniProtKB/Swiss-Prot Function]