Cwf19l1 (NM_001081077) Mouse Tagged ORF Clone

CAT#: MR208591

Cwf19l1 (Myc-DDK-tagged) - Mouse CWF19-like 1, cell cycle control (S. pombe) (Cwf19l1)

Product Images

Circular map for MR208591

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cwf19l1
• Synonyms: 2610528C06Rik; AI854304; AV336991
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208591 representing NM_001081077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGAAGCCACTGCGTCTCTTGGCCTGTGGAGATGTTGAAGGAAAGTTTGATGTTTTATTCAATA
  GAGTTCGGACAATTCAGAAGAAAAGTGGAAACTTTGATCTGCTGTTGTGTGTAGGAAACTTTTTTGGCTC
  TGCCCAGGATGCTGAGTGGGAGGAGTATAAGACCGGCAACAAGAAAGCTCCTATTCAGACATATGTGCTT
  GGTGCCAATAACGAGGAGACAGCAAACTATTTCCAGGGTGCTGATGGCTGTGAATTGGCTGAAAACATCA
  CTTACCTGGGTCGGAAAGGTGTCTTCACTGGAAGCTCGGGGCTACAGATTGTATACCTCAGTGGGACCGA
  GTCCTTAGATGAACCAGTCCCAGCCCACAGCTTTAGTCCCAAGGATGTGTCTTCTCTGAGAACAATGCTG
  TGCTCGGCTTCCCAGTTTAAAGGTGTTGATATCTTACTCACGTCCCCGTGGCCCAAGTACGTGGGGAGCT
  TTGGGAATTCCTCTGGAGAAGTAGACACCAAAAACTGTGGCTCAGCTTTGATCTCCAGCCTCGCCGTGAG
  CTTGAAACCGAGGTATCATTTTGCTGCTTTGGAAAAGTCGTATTATGAGAGGCTTCCGTACCGAAACCAT
  GTTGTTCTACAAGAAAGCGCGCAGCATGCCACTCGCTTCATAGCGCTGGCGAATGTTGGAAATCCAGAAA
  AGAAGAAGTATCTTTATGCCTTCAGTATTGTTCCCATGAAGCTGATGGCTGTAGCCGAACTGGTAAAGCA
  GCCTCCGGACGTCACCGAGAACCCTTACCGAGACTCTGGGAAGCAGGCAGCCGGAGGGAAACACATTCCC
  GCCCCTCAGGAAGAGTCAGCCTGTCAGTTCTTCTTCGATTTGAGTGAAAAGCAGGGAAGGAAGCGGCCGT
  CCACAGGCAGGGATACCAGGCCTCCCCACGCTAAGCAGCCTCGCAAGCCTCCTCAGCCTCCAGGCCCTTG
  TTGGTTTTGCCTCGCCAGCCCTGAAGTGGAAAAGCACCTGGTGGTCAACATTGGCACACATTGCTACCTT
  GCCCTGGCCAAAGGAGGCTTATCTGATGACCATGTCCTCATCCTGCCCATTGGACACTACCAGTCCGTTG
  TGGAGCTCTCGGCAGAGGTGGTGGAAGAAGTGGAGAAATATAAGGCTACTCTGCAACGCTTCTTTAAGAG
  TCGGGGGAAGCGTTGTGTTCTATTTGAAAGAAATTATAGGAGCCATCACCTCCAGCTTCAGGTCATTCCT
  GTGCCCCTCAGCTGCTGTGCTACAGATGACATTAAAGATGCCTTCATTACCCAGGCACAGGAGCAGCAGA
  TAGAGCTGTTGGAGATTCCAGAACACTCAGACATCAAACAGATTGCACAGCCAGGAGCAGCATATTTTTA
  TGTTGAACTCGACACAGGAGAAAAGCTTTTCCACAGAATTAAAAAGAATTTTCCTTTGCAATTTGGAAGG
  GAGGTCCTGGCCAGCGAAGCTATTCTCAACATTCCTGAGAAGGCTGACTGGAGGCAGTGTCAAACCAGCA
  AGGACGAGGAGGAGGCCCTGGCCCGCCGCTTCCGGAAAGACTTTGAACCCTTTGACTTCACTCTGGATGA
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208591 representing NM_001081077
  Red=Cloning site Green=Tags(s)
  
  MAQKPLRLLACGDVEGKFDVLFNRVRTIQKKSGNFDLLLCVGNFFGSAQDAEWEEYKTGNKKAPIQTYVL
  GANNEETANYFQGADGCELAENITYLGRKGVFTGSSGLQIVYLSGTESLDEPVPAHSFSPKDVSSLRTML
  CSASQFKGVDILLTSPWPKYVGSFGNSSGEVDTKNCGSALISSLAVSLKPRYHFAALEKSYYERLPYRNH
  VVLQESAQHATRFIALANVGNPEKKKYLYAFSIVPMKLMAVAELVKQPPDVTENPYRDSGKQAAGGKHIP
  APQEESACQFFFDLSEKQGRKRPSTGRDTRPPHAKQPRKPPQPPGPCWFCLASPEVEKHLVVNIGTHCYL
  ALAKGGLSDDHVLILPIGHYQSVVELSAEVVEEVEKYKATLQRFFKSRGKRCVLFERNYRSHHLQLQVIP
  VPLSCCATDDIKDAFITQAQEQQIELLEIPEHSDIKQIAQPGAAYFYVELDTGEKLFHRIKKNFPLQFGR
  EVLASEAILNIPEKADWRQCQTSKDEEEALARRFRKDFEPFDFTLDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081077
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081077.1, NP_001074546.1
• RefSeq Size: 3581 bp
• RefSeq ORF: 1614 bp
• Locus ID: 72502
• UniProt ID: Q8CI33
• Cytogenetics: 19 C3
• MW: 60.2 kDa