Tbc1d19 (NM_144517) Mouse Tagged ORF Clone

CAT#: MR208446

Tbc1d19 (Myc-DDK-tagged) - Mouse TBC1 domain family, member 19 (Tbc1d19)

Product Images

Circular map for MR208446

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tbc1d19
• Synonyms: 2810453K03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208446 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCAGGAGGAGTCGGACCTGTCCCTCATCATTGCCCAGATCGTCCAGAAACTCAAGGGCTCCAATT
  TGTATGCTCAGCTGGAACGGCAGGCCTGGTCCTGTCTTCAGAGACCCGAGATCAGACTCGAATCACTCAA
  AGAAGATATTAAGGAATTCTTTAAAATATCAGGTTGGGAGAAGAAACTTCAGAATGCTGTTTATAGTGAA
  CTGAATGTGTTTCCTTTGCCAAGTCATCCTTCTGCACCCCCTGAGCATCTCAAAGAACCTTTGGTGTACA
  TGAGGAAAGCACAGGGAAGCTGGGAAAAAAGAATCTTGAAGAGTTTAAATAGTATGTGCACAGAACTGAG
  TATCCCACTAGCACGAAAGAGACCAGCTATGGAGCAGAAAGAGCTTCTCAATAAGTGGAATGAGATGGGA
  ACGGATGAGCCAGATTTAAGCCTTTTTCGACCCGTTTATGCACCTAAGGACTTTCTTGAGGTATTAATTA
  ATCTCCGCAACCCAAATTATGAAAATGGTGATTCTCTTAGTTTTAGGACTCATTTGGGTTTAATCCAAGT
  TCCTCTGAAAGTAAAAGACATCCCTGAATTGAAAGAATGCTTTGTAGAACTTGGCTTAAACACAGGACAA
  CTGGGAATAGATGATTCTACACAAGTGCCTCCTGAACTTTTTGAAAATGAACATGTGCGTATTGGGCAGA
  AAGTTCTAGCGCAGCAGGACAGCGCTGCTGCTCAGCAGTACATCCGACAAGGAAGTCCCACAGCACTGAG
  AGCTGAGCTCTGGGCGCTCATTCTGAATATCTCCAGTCAGCCTGAGGATATCCTGTATTATGAGCAGCTT
  AAGACCAATGTGATCCAGCACGACCTTTTGGTGGACAGTCTAATTTATAAAGATGTGAAGCTGACAGCGA
  GCAATGATGATTACTATTTTGTATTTGAAGATTATTTATATCAGGTGTTACTTTGCTTTTCACGGGATAC
  GTCTGTACTGGGCCACTTTGCATACAACAGTGCTTCACCGCCGAAGTCATACATCAGAGGAAAACTAGGA
  TTAGAAGAATATGCTGTTTTCTATCCGCCCAATGGTGTCATCCCTTTCCATGGGTTTTCCATGTATGTTG
  CACCATTGTGTTTTCTGTATCATGAACCTTACAAATTGTATCAGATATTCCGTGAGATGTACGTCCGCTT
  TTTCTTCAGACTCCACTCCATCTCCTCTCATCCTTCTGGTATTGTGTCACTCTGTTTGCTGTTTGAAACT
  CTTCTTCAAACGTATCTTCCCCAACTCTTTTATCATCTGCGAGAAATTGGAGCTCAACCACTTCGGATAT
  CATTTAAATGGATGGTTCGAGCTTTTTCTGGATACTTAGCTACAGACCAACTCTTGCTTTTATGGGATAG
  AATTCTAGGATACAACTCTCTGGAAATACTTGCTGTGCTGGCAGCTGCCGTGTTTGCTTTCCGAGCAGTG
  AACCTGATGGAGGTGACATCACTGGCTGCAGCTGAAGCAGTTCTGGCTGACCTTTCTACTTTAAAAGTCA
  TGCCTCTTCTTCAAATTTTTCTGTTTGCTACCGTCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208446 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLQEESDLSLIIAQIVQKLKGSNLYAQLERQAWSCLQRPEIRLESLKEDIKEFFKISGWEKKLQNAVYSE
  LNVFPLPSHPSAPPEHLKEPLVYMRKAQGSWEKRILKSLNSMCTELSIPLARKRPAMEQKELLNKWNEMG
  TDEPDLSLFRPVYAPKDFLEVLINLRNPNYENGDSLSFRTHLGLIQVPLKVKDIPELKECFVELGLNTGQ
  LGIDDSTQVPPELFENEHVRIGQKVLAQQDSAAAQQYIRQGSPTALRAELWALILNISSQPEDILYYEQL
  KTNVIQHDLLVDSLIYKDVKLTASNDDYYFVFEDYLYQVLLCFSRDTSVLGHFAYNSASPPKSYIRGKLG
  LEEYAVFYPPNGVIPFHGFSMYVAPLCFLYHEPYKLYQIFREMYVRFFFRLHSISSHPSGIVSLCLLFET
  LLQTYLPQLFYHLREIGAQPLRISFKWMVRAFSGYLATDQLLLLWDRILGYNSLEILAVLAAAVFAFRAV
  NLMEVTSLAAAEAVLADLSTLKVMPLLQIFLFATVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144517
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144517.1
• RefSeq Size: 2688 bp
• RefSeq ORF: 1581 bp
• Locus ID: 67249
• Cytogenetics: 5 C1
• MW: 60.4 kDa