Acbd3 (NM_133225) Mouse Tagged ORF Clone

CAT#: MR208428

Acbd3 (Myc-DDK-tagged) - Mouse acyl-Coenzyme A binding domain containing 3 (Acbd3)

Product Images

Circular map for MR208428

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acbd3
• Synonyms: 60kDa; 8430407O11Rik; D1Ertd10e; GCP60; Gocap1; GOLPH1; Pap7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208428 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCAGCTGAACGTAGAGCAGCTCGAGGTGTCGCTCGACGGTCTCACGCTGAGCCCGGACTCGG
  AGGAGAGGCCCGGCGCGGAGGGGGCCCCGCCGCAGACGCCGCCGTCCTCGGCCCCGGGGAATGGACTGGG
  CTCCGGCGCCTCGGGGCAGCAGCGGGAGCCCGGGGAGGCGGCGGCTGAGGGCGCGGCCGAGGAGGCGCGG
  CGGATGGAGCAGCACTGGGGCTTTGGCCTGGAGGAGCTTTACGGCCTGGCGCTGCGCTTCTACAAAATAA
  AAGATGGCAAAGCCTTTCATCCAACTTATGAAGAAAAACTGAAGTTCGTGGCACTGCATAAGCAAGTTCT
  TTTGGGCCCATATAACCCAGACACGTCCCCTGAGGTTGGATTCTTTGATGTGTTGGGGAATGATAGGAGG
  AGAGAATGGGCAGCTCTGGGAAACATGTCCAAGGAGGATGCCATGGTAGAGTTTGTGAAGCTTCTAAATA
  AGTGTTGTCCTCTCCTCTCGGCATATGTTGCGTCCCACAGAATAGAGAAGGAAGAAGAAGAGAAAAGAAG
  AAAGGCGGAGGAGGAGCGAAGGCAGCGTGAAGAGGAAGAACGAGAGCGGCTGCAAAAGGAAGAAGAGAAG
  CGGAAGCGAGAGGAGGAAGACCGGCTGAGACGGGAGGAGGAAGAGAGGCGGCGGATAGAGGAAGAGAGGC
  TTCGGCTGGAACAGCAAAAGCAGCAGATAATGGCAGCTTTAAACTCGCAGACTGCCGTGCAATTCCAGCA
  GTATGCAGCCCAGCAGTATCCAGGGAACTACGAACAACAGCAGATTCTCATCCGCCAGCTGCAGGAGCAG
  CACTATCAGCAGTATATGCAGCAGTTATATCAAGTCCAGCTTGCACAACAACAGGCAGCATTACAGAAAC
  AGCAAGAAGTAGTGATGGCTGGGGCATCATTGCCTGCATCATCAAAGGTGAACACAGTTGGAGCAAGTGA
  TACACTGTCAGTTAATGGACAGGCCAAAACCCACACTGAAAATTCCGAAAAAGTCCTTGAGCCAGAAGCT
  GCAGAAGAAGCCTTGGAAAATGGACCAAAAGACTCTCTTCCAGTGATTGCAGCTCCATCCATGTGGACAA
  GACCACAAATCAAAGACTTTAAAGAGAAGATTCGGCAGGATGCAGATTCTGTGATTACAGTACGTCGAGG
  AGAAGTCGTCACCGTCCGAGTCCCGACTCATGAGGAAGGATCATACCTATTTTGGGAATTTGCCACAGAC
  AGTTATGACATTGGGTTTGGGGTTTATTTTGAATGGACAGACTCTCCAAATGCTGCTGTCAGTGTGCATG
  TCAGTGAGTCCAGTGACGAGGAGGAGGAGGAGGAAGAAAATGTCACTTGTGAAGAAAAAGCAAAAAAGAA
  CGCCAACAAGCCTCTGCTGGATGAGATTGTACCTGTGTACCGGCGGGACTGTCACGAGGAAGTATATGCA
  GGCAGCCACCAGTATCCAGGGAGGGGAGTCTATCTCCTCAAGTTTGATAATTCCTACTCTCTGTGGAGGT
  CCAAGTCCGTCTACTACAGAGTCTATTATACTAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208428 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAQLNVEQLEVSLDGLTLSPDSEERPGAEGAPPQTPPSSAPGNGLGSGASGQQREPGEAAAEGAAEEAR
  RMEQHWGFGLEELYGLALRFYKIKDGKAFHPTYEEKLKFVALHKQVLLGPYNPDTSPEVGFFDVLGNDRR
  REWAALGNMSKEDAMVEFVKLLNKCCPLLSAYVASHRIEKEEEEKRRKAEEERRQREEEERERLQKEEEK
  RKREEEDRLRREEEERRRIEEERLRLEQQKQQIMAALNSQTAVQFQQYAAQQYPGNYEQQQILIRQLQEQ
  HYQQYMQQLYQVQLAQQQAALQKQQEVVMAGASLPASSKVNTVGASDTLSVNGQAKTHTENSEKVLEPEA
  AEEALENGPKDSLPVIAAPSMWTRPQIKDFKEKIRQDADSVITVRRGEVVTVRVPTHEEGSYLFWEFATD
  SYDIGFGVYFEWTDSPNAAVSVHVSESSDEEEEEEENVTCEEKAKKNANKPLLDEIVPVYRRDCHEEVYA
  GSHQYPGRGVYLLKFDNSYSLWRSKSVYYRVYYTR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133225
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133225.1, NM_133225.2, NM_133225.3, NP_573488.1
• RefSeq Size: 3487 bp
• RefSeq ORF: 1578 bp
• Locus ID: 170760
• UniProt ID: Q8BMP6
• Cytogenetics: 1 84.46 cM
• MW: 60.2 kDa
• Gene Summary: Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi (By similarity). Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (PubMed:12711385). Recruits PI4KB to the Golgi apparatus membrane; enhances the enzyme activity of PI4KB activity via its membrane recruitment thereby increasing the local concentration of the substrate in the vicinity of the kinase (By similarity).[UniProtKB/Swiss-Prot Function]