Glyctk (NM_174846) Mouse Tagged ORF Clone

CAT#: MR208380

Glyctk (Myc-DDK-tagged) - Mouse glycerate kinase (Glyctk), transcript variant 1

Product Images

Circular map for MR208380

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glyctk
• Synonyms: 6230410P16Rik; C430006L12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208380 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCCCTACAGGTCTTGCCCTGTCTCCTCCGAGCCCCGTCCCGTCCACTCCTCTGGGGGCCCC
  CAGTGGCCCGGATGACCAGTGGTATGGCTTTGGCAGAACAGGCACGGCAGCTGTTTGATAGTGCAGTGGG
  TGCTGTGCAGCCAGGCCCCATGCTGCAGAGGACACTGTCCCTGGATCCTAGTGGCAGACAGCTGAAGGTG
  CGGGACCGGACCTTCCAGCTGCGGGAAAACCTCTACCTAGTGGGCTTCGGCAAGGCCGTACTGGGCATGG
  CAGCTGCTGCCGAGGAGCTGCTGGCTCAGCATCTTGTGCAGGGTGTGATCAGCGTACCCAAGGGGATCCG
  AGCCGCTATGGAACATGCTGGAAAGAAAGAGATGCTGCTGAAGCCACACAGCCGCGTCCAGGTGTTCGAG
  GGCGCCGAGGACAACTTACCGGATCGGGATGCACTCCGGGCCGCGCTGACCATCCAGCAGCTGGCAGAGG
  GGCTCACTGCAGATGACCTGCTGCTGGTGCTCATCTCAGGTGGGGGCTCAGCCCTGCTGCCTGCTCCAAT
  CCCACCTGTCACACTGGAGGAGAAACAGATGCTCACCAAGCTGCTGGCAGCCCGAGGAGCCACGATCCAG
  GAGCTGAACACCATCCGCAAGGCCCTGTCGCAGCTGAAGGGTGGAGGGCTGGCTCAGGCTGCCTATCCTG
  CTCAGGTGATAAGCCTCATCTTGTCAGATGTGATTGGGGACCCTTTGGAAGTGATTGCCAGTGGCCCTAC
  GGTGGCCAGCGCCCACAGTGTGCAAGACTGCCTGCATATTCTCAACCACTATGGCCTCCGTGCTGCTCTT
  CCCCGCTCTGTGAAGACTGTGCTCTCCCGAGCTGACTCTGACCCCCATGGACCACACACCTGTGGCCACG
  TCCTCAATGTGATCATCGGCTCTAACTCTCTGGCGCTGGCTGAGGCTCAGAGGCAAGCTGAGGTGCTGGG
  CTACCATGCCATGGTGTTGAGCACAGCCATGCAGGGTGATGTAAAAAGAGTGGCTCGGTTCTACGGGCTG
  CTAGCCCGGGTGGCTGCAGCCCACCTCACTCCATCTTTGGCTGAGCGTCCCTTGGAGGAGGAGGCGGAAC
  TCCATCAGCTGGCAGCTGAGCTCCAGCTCCCGGACCTGCAGCTGGAGGAGGCTCTGGAAGCGGTGGCAAA
  GGCTAAAGGGCCGGTCTGCCTCCTGGCTGGTGGTGAGCCCACGGTGCAGCTGCAGGGATCTGGCAAGGGC
  GGTCGGAACCAAGAACTGGCCTTGCACGTGGGAGTGGAGCTGGGAAGGCAGCCACTGGGACCTATTGATG
  TGCTATTTTTGAGTGGAGGCACTGATGGGCAGGATGGGCCTACAAAGGTGGCCGGGGCCTGGGTCATGTC
  TGATCTCATCAGCCAGGCTTCTGCTGAGAGCCTGGACATCGCCACCTCCCTCACCAACAACGACTCATAC
  ACTTTCTTTTGCCGCTTCCGGGGAGGAACACATTTGCTGCACACAGGGTTGACAGGGACCAATGTTATGG
  ATGTCCACCTCCTGATTCTTCATCCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208380 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAALQVLPCLLRAPSRPLLWGPPVARMTSGMALAEQARQLFDSAVGAVQPGPMLQRTLSLDPSGRQLKV
  RDRTFQLRENLYLVGFGKAVLGMAAAAEELLAQHLVQGVISVPKGIRAAMEHAGKKEMLLKPHSRVQVFE
  GAEDNLPDRDALRAALTIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQMLTKLLAARGATIQ
  ELNTIRKALSQLKGGGLAQAAYPAQVISLILSDVIGDPLEVIASGPTVASAHSVQDCLHILNHYGLRAAL
  PRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNSLALAEAQRQAEVLGYHAMVLSTAMQGDVKRVARFYGL
  LARVAAAHLTPSLAERPLEEEAELHQLAAELQLPDLQLEEALEAVAKAKGPVCLLAGGEPTVQLQGSGKG
  GRNQELALHVGVELGRQPLGPIDVLFLSGGTDGQDGPTKVAGAWVMSDLISQASAESLDIATSLTNNDSY
  TFFCRFRGGTHLLHTGLTGTNVMDVHLLILHPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174846
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_174846.3
• RefSeq Size: 4226 bp
• RefSeq ORF: 1572 bp
• Locus ID: 235582
• UniProt ID: Q8QZY2
• Cytogenetics: 9 F1
• MW: 55.3 kDa