Atp6v1b1 (NM_134157) Mouse Tagged ORF Clone

CAT#: MR208239

Atp6v1b1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1)

Product Images

Circular map for MR208239

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp6v1b1
• Synonyms: Atp6b1; AW208839; D630003L15; D630030L16Rik; D630039P21Rik; Vpp-3; Vpp3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208239 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACAACAGTAGACAGCAGGTCCTCGGGATTCACTGGCAATAGCTGTGACCCAGGCACAGCCCAAG
  AGCATGTGCAGGCTGTGACCCGAAACTACATCACCCACCCCCGAGTCACCTATAGGACAGTGTGCAGCGT
  CAATGGGCCTCTGGTGGTGCTGGACCAGGTCAAGTTTGCCCAGTATGCTGAGATTGTCAACTTTACCCTC
  CCTGATGGTACTCAGAGGAGTGGCCAGGTGCTTGAAGTGGCTGGGACCAAGGCCATTGTTCAGGTGTTTG
  AAGGGACTTCAGGGATTGATTCCCAGAAGACCACATGTGAATTCACAGGGGACATCCTGCGGACTCCTGT
  GTCAGAGGACATGCTGGGTCGGATTTTCAATGGCTCTGGCAAACCCATTGACAAAGGACCTGCTGTCATG
  GCAGAGGAGTTTCTGGACATCAACGGCCAGCCAATCAATCCCCATGACCGAATCTACCCCGAGGAGATGA
  TCCAGACGGGAATCTCCCCCATTGATGTCATGAACAGCATCGCTCGTGGCCAGAAGATCCCCATCTTCTC
  TGCGGCTGGGCTCCCCCACAATGAGATTGCAGCCCAGATCTGCCGCCAAGCCGGGCTGGTGAAGAAATCC
  AAGGCTGTGCTGGATTATCATGAAGACAACTTCGCCATTGTCTTTGCAGCCATGGGGGTGAACATGGAGA
  CAGCCAGGTTCTTCAAGTCAGATTTCGAGCAGAATGGAACCATGGGAAATGTCTGTCTCTTCCTGAATTT
  GGCCAATGACCCTACGATTGAGCGGATCATCACGCCACGTCTGGCACTGACCACGGCTGAGTTCCTTGCC
  TACCAGTGTGAGAAGCACGTGCTGGTGATACTGACTGACATGAGCTCCTATGCGGAGGCCTTGCGGGAGG
  TCTCAGCTGCCCGGGAGGAAGTGCCAGGACGCCGTGGCTTTCCTGGATATATGTACACAGACTTGGCCAC
  CATCTATGAGCGAGCTGGCCGTGTGGAGGGCCGGGGTGGTTCCATCACCCAGATCCCCATTCTCACCATG
  CCTAATGATGATATCACCCATCCCATCCCAGACTTGACTGGCTTCATCACAGAGGGGCAGATTTATGTGG
  ACCGACAGCTACATAACCGGCAGGTCTACCCACCCATCAATGTGCTCCCATCCCTCTCGCGACTGATGAA
  GTCGGCCATAGGCGAGGGCATGACCAGAAAGGACCACGGAGATGTTTCCAACCAGCTGTACGCCTGCTAC
  GCCATCGGGAAGGACGTGCAGGCCATGAAAGCGGTGGTGGGGGAGGAGGCGCTCACCTCCGAGGACCTGC
  TCTACCTGGAGTTCCTGCAGAAGTTTGAGAAGAATTTCATCACCCAAGGTCCTTACGAGAACCGCACGGT
  GTTCGAGTCGCTGGACCTGGGCTGGAAACTGCTGCGCATCTTTCCGAAAGAGATGCTGAAGCGCATTCCG
  CAGAGCATGACGGACGAGTTCTACTCTCGCCAGGGGGCGCAGCAGGACCCCGCATCCGACACGGCGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208239 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATTVDSRSSGFTGNSCDPGTAQEHVQAVTRNYITHPRVTYRTVCSVNGPLVVLDQVKFAQYAEIVNFTL
  PDGTQRSGQVLEVAGTKAIVQVFEGTSGIDSQKTTCEFTGDILRTPVSEDMLGRIFNGSGKPIDKGPAVM
  AEEFLDINGQPINPHDRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKS
  KAVLDYHEDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLA
  YQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRGGSITQIPILTM
  PNDDITHPIPDLTGFITEGQIYVDRQLHNRQVYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYACY
  AIGKDVQAMKAVVGEEALTSEDLLYLEFLQKFEKNFITQGPYENRTVFESLDLGWKLLRIFPKEMLKRIP
  QSMTDEFYSRQGAQQDPASDTAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134157
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134157.1
• RefSeq Size: 1945 bp
• RefSeq ORF: 1542 bp
• Locus ID: 110935
• UniProt ID: Q91YH6
• Cytogenetics: 6 35.94 cM
• MW: 56.8 kDa
• Gene Summary: Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:16174750, PubMed:23028982). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Essential for the proper assembly and activity of V-ATPase (By similarity). In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed:16174750). Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (PubMed:23028982).[UniProtKB/Swiss-Prot Function]