Atp6v1b1 (NM_134157) Mouse Tagged ORF Clone

CAT#: MG208239

Atp6v1b1 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit B1 (Atp6v1b1)

Product Images

Circular map for MG208239

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp6v1b1
• Synonyms: Atp6b1; AW208839; D630003L15; D630030L16Rik; D630039P21Rik; Vpp-3; Vpp3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208239 representing NM_134157
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACAACAGTAGACAGGTCCTCGGGATTCACTGGCAATAGCTGTGACCCAGGCACAGCCCAAGAGC
  ATGTGCAGGCTGTGACCCGAAACTACATCACCCACCCACGAGTCACCTATAGGACAGTGTGCAGCGTCAA
  TGGGCCTCTGGTGGTGCTGGACCAGGTCAAGTTTGCCCAGTATGCTGAGATTGTCAACTTTACCCTCCCT
  GATGGTACTCAGAGGAGTGGCCAGGTGCTTGAAGTGGCTGGGACCAAGGCCATTGTTCAGGTGTTTGAAG
  GGACTTCAGGGATTGATTCCCAGAAGACCACATGTGAATTCACAGGGGACATCCTGCGGACTCCTGTGTC
  AGAGGACATGCTGGGTCGGATTTTCAATGGCTCTGGCAAACCCATTGACAAAGGACCTGCTGTCATGGCA
  GAGGAGTTTCTGGACATCAATGGCCAGCCAATCAATCCCCATGACCGAATCTACCCCGAGGAGATGATCC
  AGACGGGAATCTCCCCCATTGATGTCATGAACAGCATCGCTCGTGGCCAGAAGATCCCCATCTTCTCTGC
  GGCTGGGCTCCCCCACAATGAGATTGCAGCCCAGATCTGCCGCCAAGCCGGGCTGGTGAAGAAATCCAAG
  GCTGTGCTGGATTATCATGAAGACAACTTCGCCATTGTCTTTGCAGCCATGGGGGTGAACATGGAGACAG
  CCAGGTTCTTCAAGTCAGATTTCGAGCAGAATGGAACCATGGGAAATGTCTGTCTCTTCCTGAATTTGGC
  CAATGACCCTACGATTGAGCGGATCATCACGCCACGTCTGGCACTGACCACGGCTGAGTTCCTTGCCTAC
  CAGTGTGAGAAGCACGTGCTGGTGATACTGACTGACATGAGCTCCTATGCGGAGGCCTTGCGGGAGGTCT
  CAGCTGCCCGGGAGGAAGTGCCAGGACGCCGTGGCTTTCCTGGATATATGTACACAGACTTGGCCACCAT
  CTATGAGCGAGCTGGCCGTGTGGAGGGCCGGGGTGGTTCCATCACCCAGATCCCCATTCTCACCATGCCT
  AATGATGATATCACCCATCCCATCCCAGACTTGACTGGCTTCATCACAGAGGGGCAGATTTATGTGGACC
  GACAGCTACATAACCGGCAGGTCTACCCACCCATCAATGTGCTCCCATCCCTCTCGCGACTGATGAAGTC
  GGCCATAGGCGAGGGCATGACCAGAAAGGACCACGGAGATGTCTCCAACCAGCTGTACGCCTGCTACGCC
  ATCGGGAAGGACGTGCAGGCCATGAAAGCGGTGGTGGGGGAGGAGGCGCTCACCTCCGAGGACCTGCTCT
  ACCTGGAGTTCCTGCAGAAGTTTGAGAAGAATTTCATCACCCAAGGTCCTTACGAGAACCGCACGGTGTT
  CGAGTCGCTGGACCTGGGCTGGAAACTGCTGCGCATCTTTCCGAAAGAGATGCTGAAGCGCATTCCGCAG
  AGCATGACGGACGAGTTCTACTCTCGCCAGGGGGCGCAGCAGGACCCCGCATCCGACACGGCGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208239 representing NM_134157
  Red=Cloning site Green=Tags(s)
  
  MATTVDRSSGFTGNSCDPGTAQEHVQAVTRNYITHPRVTYRTVCSVNGPLVVLDQVKFAQYAEIVNFTLP
  DGTQRSGQVLEVAGTKAIVQVFEGTSGIDSQKTTCEFTGDILRTPVSEDMLGRIFNGSGKPIDKGPAVMA
  EEFLDINGQPINPHDRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSK
  AVLDYHEDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLAY
  QCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRGGSITQIPILTMP
  NDDITHPIPDLTGFITEGQIYVDRQLHNRQVYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYACYA
  IGKDVQAMKAVVGEEALTSEDLLYLEFLQKFEKNFITQGPYENRTVFESLDLGWKLLRIFPKEMLKRIPQ
  SMTDEFYSRQGAQQDPASDTAL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134157
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134157.2, NP_598918.1
• RefSeq Size: 1945 bp
• RefSeq ORF: 1542 bp
• Locus ID: 110935
• UniProt ID: Q91YH6
• Cytogenetics: 6 35.94 cM
• Gene Summary: Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:16174750, PubMed:23028982). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (By similarity). Essential for the proper assembly and activity of V-ATPase (By similarity). In renal intercalated cells, mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification (PubMed:16174750). Required for optimal olfactory function by mediating the acidification of the nasal olfactory epithelium (PubMed:23028982).[UniProtKB/Swiss-Prot Function]