Chrna2 (NM_144803) Mouse Tagged ORF Clone

CAT#: MR208228

Chrna2 (Myc-DDK-tagged) - Mouse cholinergic receptor, nicotinic, alpha polypeptide 2 (neuronal) (Chrna2)

Product Images

Circular map for MR208228

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chrna2
• Synonyms: Acra-2; Acra2; BC011490
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208228 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCTCCCATCCTGCTTTCCAGTTCTGGATACATCTTTATCTCTGGTGTCTGCTTCTGATGCCAG
  CAGTGTTGGCCCAGCAAGGCTCGCACACCCATGCCGAGGACCGCCTGTTCAAACACCTCTTTGGAGGCTA
  CAATCGCTGGGCACGGCCAGTGCCCAACACTTCCGATGTGGTCATCGTGCGCTTTGGACTATCCATTGCA
  CAGCTCATAGATGTGGATGAGAAGAATCAAATGATGACCACCAATGTCTGGCTAAAGCAGGAATGGAATG
  ACTACAAGCTGCGCTGGGACCCGGCTGAGTTTGGCAACATCACCTCCCTCCGCGTCCCCTCAGAGATGAT
  CTGGATCCCAGACATTGTTCTCTACAACAATGCAGACGGGGAGTTTGCGGTGACCCACATGACCAAGGCC
  CACCTCTTCTTCACGGGCACTGTGCACTGGGTGCCCCCAGCCATCTACAAGAGCTCCTGCAGCATCGACG
  TGACTTTCTTCCCCTTCGACCAGCAGAACTGCAAGATGAAGTTTGGCTCCTGGACATACGACAAGGCCAA
  GATCGACCTGGAGCAGATGGAGCGGACGGTGGACCTGAAGGACTACTGGGAGAGCGGCGAGTGGGCCATT
  ATCAACGCCACCGGAACCTATAACAGCAAGAAGTACGACTGCTGCGCGGAGATCTACCCCGACGTCACCT
  ACTACTTCGTTATCCGCCGGCTGCCGCTGTTCTACACCATCAACCTCATTATCCCGTGCCTGCTCATCTC
  CTGCCTCACCGTGCTCGTGTTCTACTTGCCCTCTGAGTGTGGAGAGAAGATCACGCTGTGCATCTCGGTG
  CTGCTCTCCCTCACTGTCTTCCTGCTGCTCATCACAGAAATTATCCCATCCACCTCACTGGTCATCCCAC
  TCATCGGCGAGTACCTGCTCTTCACCATGATCTTCGTCACCCTCTCTATTGTGATCACAGTCTTCGTGCT
  CAATGTCCACCACCGTTCCCCTAGCACGCACAACATGCCCAACTGGGTGAGGGTGGCCCTGCTAGGCCGG
  GTGCCCCGGTGGCTGATGATGAATCGGCCCCTGCCGCCCATGGAGCTCCATGGCTCTCCAGGTCTGAAGC
  TCAGCCCCACTTATCATTGGCTGGAGACCAACATGGATGCTGAAGAAAGGGAGGAGACAGAGGAAGAGGA
  GGAGGAGGAAGAAGATGAAAACATATGTATGTGTGCAGGCCTCCCAGACTCTTCCATGGGTGTCCTCTAT
  GGCCATGGCAGCCTGCATCTGAGGGCCATGGGGCCTGAGGCCAAGACTCCATCCCAGGCTAGTGAGATCC
  TGCTGTCACCTCAAATACAGAAAGCACTAGAAGGTGTACACTACATTGCTGACCACCTGAGGTCTGAGGA
  TGCTGACTCTTCGGTGAAGGAAGATTGGAAGTATGTGGCCATGGTGGTAGACCGGATCTTCCTCTGGCTG
  TTCATTATCGTCTGCTTCCTGGGGACCATCGGGCTCTTCCTCCCTCCGTTCCTGGCTGGAATGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208228 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPSHPAFQFWIHLYLWCLLLMPAVLAQQGSHTHAEDRLFKHLFGGYNRWARPVPNTSDVVIVRFGLSIA
  QLIDVDEKNQMMTTNVWLKQEWNDYKLRWDPAEFGNITSLRVPSEMIWIPDIVLYNNADGEFAVTHMTKA
  HLFFTGTVHWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLEQMERTVDLKDYWESGEWAI
  INATGTYNSKKYDCCAEIYPDVTYYFVIRRLPLFYTINLIIPCLLISCLTVLVFYLPSECGEKITLCISV
  LLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHRSPSTHNMPNWVRVALLGR
  VPRWLMMNRPLPPMELHGSPGLKLSPTYHWLETNMDAEEREETEEEEEEEEDENICMCAGLPDSSMGVLY
  GHGSLHLRAMGPEAKTPSQASEILLSPQIQKALEGVHYIADHLRSEDADSSVKEDWKYVAMVVDRIFLWL
  FIIVCFLGTIGLFLPPFLAGMI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144803
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144803.2, NP_659052.1
• RefSeq Size: 3527 bp
• RefSeq ORF: 1539 bp
• Locus ID: 110902
• UniProt ID: Q91X60
• Cytogenetics: 14 34.36 cM
• MW: 58.7 kDa
• Gene Summary: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.[UniProtKB/Swiss-Prot Function]