Gdap2 (NM_010269) Mouse Tagged ORF Clone

CAT#: MR207990

Gdap2 (Myc-DDK-tagged) - Mouse ganglioside-induced differentiation-associated-protein 2 (Gdap2)

Product Images

Circular map for MR207990

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gdap2
• Synonyms: C77050; D3Ertd801e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207990 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCTTGGGTGCACCTTCCCAGTTTGTGGATGTGGATACCCTGCTAAGCTGGGGCGACTCGTACG
  AAGATGAAGTAGACTGCGCTGACAGTACTGCCGAGGCCTTCCAGGAAGACGCCAGCAGATCACCTTTTGT
  TTATAGTCGGGACGTCAATGGAAAAGTGGTTCTTTGGAAAGGAGATGTGGCATTACTGAACTGCACAGCC
  ATTGTGAATACCAGCAATGAAAGCCTCACAGATAAGAACCCTGTGTCAGAGAGCATCTTCATGCTTGCGG
  GGCCAGATTTGAAGGAGGACCTTCAGAAACTCAAAGGTTGCCGAACTGGTGAAGCAAAATTGACGAAAGG
  CTTTAACCTAGCTGCTCGGTTCATCATTCACACAGTGGGACCTAAGTACAAGAGCCGCTACCGCACTGCA
  GCGGAGAGTTCCCTGTACAGCTGTTACAGAAACGTGCTTCAGCTGGCAAAAGAACAGTCCATGTCTTCTG
  TTGGCTTCTGTGTCATCAATTCTGCCAAGCGAGGTTATCCACTAGAAGATGCAACACACATCGCTCTTCG
  TACTGTGAGGCGGTTCCTAGAGATCCATGGGGAAAACATTGAAAAAGTAGTATTTGCTGTCTCAGAACTC
  GAAGAGGCCACTTACCAAAAGCTGCTACCTCTGTACTTCCCAAGGTCATTAAAGGAGGAGATCCGGTCAT
  TGCCGTACCTACCTGCAGACATTGGAAATGCAGAAGGGGAGCCCGTGGTGCCCGAGCGGCAAATTAGAAT
  CAGTGAGAAACCCGGTGCCTCGGAGGACAATGAAGAGGAAGATGAGGATGAAGGCTTGGGAGTGGATCTG
  TCTTTTATTGGCTCTCATGCTTTTGCTCGGATGGAAGGAGATATTGACAAGCAAAGAAAGTTGATCCTCC
  AGGGACAGTTGTCTGAAGCCGCCCTGCAGAAGCAGCATCAAAGAAATTATAATCGCTGGTTATGCCAAGC
  AAGATCTGAAGACCTGTCTGATATTGCTTCTCTGAAAGCTTTATACCAAACAGGGGTAGATAACTGTGGC
  CGCACAGTGATGGTGGTAGTTGGAAGAAACATTCCTGTGACACTGATAGATATGGACAAGGCCCTCTTGT
  ATTTTATCCATGTAATGGATCACATTGCTGTGAAGGAGTATGTGCTGGTGTATTTCCACACACTGACCAG
  CGACTACAATCACCTGGACTCCGACTTCCTGAAGAAACTCTACGATGTGGTTGACATCAAATACAAGAGG
  AATTTGAAGGCTGTTTATTTCGTTCATCCAACATTCCGTTCAAAGGTCTCAACATGGTTTTTCACCACCT
  TTTCTGTCTCAGGACTGAAGGACAAGATCCACCACGTAGACAGCCTGCAGCAGCTCTTCTCTGCCATATC
  ACCAGAGCAGATAGACTTTCCTCCTTTTGTCCTTGAATATGACGCGAGGGAAAATGGGCCTTATTTTGCA
  TCTTATCCTCCATCACCAGATTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207990 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPLGAPSQFVDVDTLLSWGDSYEDEVDCADSTAEAFQEDASRSPFVYSRDVNGKVVLWKGDVALLNCTA
  IVNTSNESLTDKNPVSESIFMLAGPDLKEDLQKLKGCRTGEAKLTKGFNLAARFIIHTVGPKYKSRYRTA
  AESSLYSCYRNVLQLAKEQSMSSVGFCVINSAKRGYPLEDATHIALRTVRRFLEIHGENIEKVVFAVSEL
  EEATYQKLLPLYFPRSLKEEIRSLPYLPADIGNAEGEPVVPERQIRISEKPGASEDNEEEDEDEGLGVDL
  SFIGSHAFARMEGDIDKQRKLILQGQLSEAALQKQHQRNYNRWLCQARSEDLSDIASLKALYQTGVDNCG
  RTVMVVVGRNIPVTLIDMDKALLYFIHVMDHIAVKEYVLVYFHTLTSDYNHLDSDFLKKLYDVVDIKYKR
  NLKAVYFVHPTFRSKVSTWFFTTFSVSGLKDKIHHVDSLQQLFSAISPEQIDFPPFVLEYDARENGPYFA
  SYPPSPDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010269
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010269.3
• RefSeq Size: 2934 bp
• RefSeq ORF: 1497 bp
• Locus ID: 14547
• UniProt ID: Q9DBL2
• Cytogenetics: 3 F2.2
• MW: 56.3 kDa