Pepd (NM_008820) Mouse Tagged ORF Clone

CAT#: MR207914

Pepd (Myc-DDK-tagged) - Mouse peptidase D (Pepd)

Product Images

Circular map for MR207914

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pepd
• Synonyms: dal; Pep-4; Pep4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207914 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCACTGTGCGCCCTTCCTTCTCCCTGGGGAATGAGACCCTGAAGGTGCCACTGGCACTATTCG
  CCCTGAACAGGCAGCGCCTGTGTGAGAGGCTGAGGAAGAATGGAGCTGTACAGGCCGCCTCAGCTGTCGT
  GTTGCAGGGCGGGGAAGAGATGCAACGGTACTGCACCGATACCTCCATCATCTTCCGCCAGGAGTCCTTC
  TTTCACTGGGCCTTCGGTGTCGTCGAGTCAGGCTGCTATGGTGTCATCGATGTGGACACTGGAAAATCAA
  CCCTGTTTGTGCCCAGGCTTCCTGATAGCTACGCCACCTGGATGGGAAAGATCCATTCCAAGGAGTATTT
  CAAGGAGAAGTACGCTGTGGATGATGTCCAGTACACGGATGAGATTGCCAGTGTCCTGACCTCACGGAAC
  CCCTCTGTCCTCCTCACTTTGCGCGGTGTCAACACAGACAGTGGCAGTGTCTGCAGGGAGGCCTCCTTCG
  AGGGCATTAGCAAGTTCAATGTCAACAACACGATTCTTCACCCAGAGATCGTGGAATGCCGAGTGTTCAA
  GACAGACATGGAACTAGAGGTTTTGCGCTACACAAATCGGATCTCCAGCGAGGCCCACCGTGAGGTAATG
  AAGGCTGTAAAAGTGGGGATGAAGGAATACGAGATGGAAAGCCTGTTCCAGCATTATTGTTACTCTAGGG
  GCGGCATGCGCCACACCTCTTACACCTGTATCTGCTGTAGCGGTGAGAACGCGGCCGTGCTGCACTATGG
  CCATGCTGGAGCCCCCAATGACCGGACGATCAAGGATGGAGATATTTGCCTGTTCGACATGGGTGGCGAG
  TACTACTGCTTTGCTTCTGACATCACCTGCTCCTTCCCAGCCAACGGCAAGTTCACAGAGGACCAGAAGG
  CCATCTACGAAGCAGTGCTCAGGAGCTGCAGAACTGTCATGAGCACTATGAAGCCAGGGGTCTGGTGGCC
  TGATATGCATCGGCTGGCTGACCGTATCCACCTGGAGGAACTGGCACGCATTGGCCTCCTGAGTGGCAGT
  GTGGATGCCATGCTCCAGGTCCACCTGGGAGCAGTATTCATGCCACATGGACTTGGCCACTTTCTGGGCC
  TAGATGTGCACGACGTGGGAGGCTACCCTGAGGGTGTAGAGCGCATCGATGAACCTGGCCTGCGAAGCCT
  GCGTACGGCAAGGCACCTGGAGCCGGGCATGGTGCTGACTGTGGAGCCAGGCATCTACTTCATTGACCAC
  CTCTTGGACCAGGCCCTGGCAGACCCGGCCCAAGCCTGCTTCTTTAACCAAGAGGTCCTGCAGCGCTTCC
  GCAACTTCGGTGGGGTGCGGATCGAGGAAGATGTTGTCGTGACTGACAGCGGCATGGAGCTGCTGACATG
  TGTACCCCGGACTGTGGAGGAAATCGAAGCGTGCATGGCAGGCTGCGACAAGGCATCTGTGCCCTTCTCT
  GGCCAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207914 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASTVRPSFSLGNETLKVPLALFALNRQRLCERLRKNGAVQAASAVVLQGGEEMQRYCTDTSIIFRQESF
  FHWAFGVVESGCYGVIDVDTGKSTLFVPRLPDSYATWMGKIHSKEYFKEKYAVDDVQYTDEIASVLTSRN
  PSVLLTLRGVNTDSGSVCREASFEGISKFNVNNTILHPEIVECRVFKTDMELEVLRYTNRISSEAHREVM
  KAVKVGMKEYEMESLFQHYCYSRGGMRHTSYTCICCSGENAAVLHYGHAGAPNDRTIKDGDICLFDMGGE
  YYCFASDITCSFPANGKFTEDQKAIYEAVLRSCRTVMSTMKPGVWWPDMHRLADRIHLEELARIGLLSGS
  VDAMLQVHLGAVFMPHGLGHFLGLDVHDVGGYPEGVERIDEPGLRSLRTARHLEPGMVLTVEPGIYFIDH
  LLDQALADPAQACFFNQEVLQRFRNFGGVRIEEDVVVTDSGMELLTCVPRTVEEIEACMAGCDKASVPFS
  GQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008820
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008820.2, NP_032846.2
• RefSeq Size: 1856 bp
• RefSeq ORF: 1482 bp
• Locus ID: 18624
• UniProt ID: Q11136
• Cytogenetics: 7 20.7 cM
• MW: 55 kDa
• Gene Summary: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because of the high level of iminoacids in collagen.[UniProtKB/Swiss-Prot Function]