Pepd (NM_008820) Mouse Untagged Clone

CAT#: MC216809

Pepd (untagged) - Mouse peptidase D (Pepd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pepd
• Synonyms: dal; Pep-4; Pep4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216809 representing NM_008820
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCACTGTGCGCCCTTCCTTCTCCCTGGGGAATGAGACCCTGAAGGTGCCACTGGCACTATTCG
  CCCTGAACAGGCAGCGCCTGTGTGAGAGGCTGAGGAAGAATGGAGCTGTACAGGCCGCCTCAGCTGTCGT
  GTTGCAGGGCGGGGAAGAGATGCAACGGTACTGCACCGATACCTCCATCATCTTCCGCCAGGAGTCCTTC
  TTTCACTGGGCCTTCGGTGTCGTCGAGTCAGGCTGCTATGGTGTCATCGATGTGGACACTGGAAAATCAA
  CCCTGTTTGTGCCCAGGCTTCCTGATAGCTACGCCACCTGGATGGGAAAGATCCATTCCAAGGAGTATTT
  CAAGGAGAAGTACGCTGTGGATGATGTCCAGTACACGGATGAGATTGCCAGTGTCCTGACCTCACGGAAC
  CCCTCTGTCCTCCTCACTTTGCGCGGTGTCAACACAGACAGTGGCAGTGTCTGCAGGGAGGCCTCCTTCG
  AGGGCATTAGCAAGTTCAATGTCAACAACACGATTCTTCACCCAGAGATCGTGGAATGCCGAGTGTTCAA
  GACAGACATGGAACTAGAGGTTTTGCGCTACACAAATCGGATCTCCAGCGAGGCCCACCGTGAGGTAATG
  AAGGCTGTAAAAGTGGGGATGAAGGAATACGAGATGGAAAGCCTGTTCCAGCATTATTGTTACTCTAGGG
  GCGGCATGCGCCACACCTCTTACACCTGTATCTGCTGTAGCGGTGAGAACGCGGCCGTGCTGCACTATGG
  CCATGCTGGAGCCCCCAATGACCGGACGATCAAGGATGGAGATATTTGCCTGTTCGACATGGGTGGCGAG
  TACTACTGCTTTGCTTCTGACATCACCTGCTCCTTCCCAGCCAACGGCAAGTTCACAGAGGACCAGAAGG
  CCATCTACGAAGCAGTGCTCAGGAGCTGCAGAACTGTCATGAGCACTATGAAGCCAGGGGTCTGGTGGCC
  TGATATGCATCGGCTGGCTGACCGTATCCACCTGGAGGAACTGGCACGCATTGGCCTCCTGAGTGGCAGT
  GTGGATGCCATGCTCCAGGTCCACCTGGGAGCAGTATTCATGCCACATGGACTTGGCCACTTTCTGGGCC
  TAGATGTGCACGACGTGGGAGGCTACCCTGAGGGTGTAGAGCGCATCGATGAACCTGGCCTGCGAAGCCT
  GCGTACGGCAAGGCACCTGGAGCCGGGCATGGTGCTGACTGTGGAGCCAGGCATCTACTTCATTGACCAC
  CTCTTGGACCAGGCCCTGGCAGACCCGGCCCAAGCCTGCTTCTTTAACCAAGAGGTCCTGCAGCGCTTCC
  GCAACTTCGGTGGGGTGCGGATCGAGGAAGATGTTGTCGTGACTGACAGCGGCATGGAGCTGCTGACATG
  TGTACCCCGGACTGTGGAGGAAATCGAAGCGTGCATGGCAGGCTGCGACAAGGCATCTGTGCCCTTCTCT
  GGCCAAAAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008820
• Insert Size: 1482 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008820.2, NP_032846.2
• RefSeq Size: 1856 bp
• RefSeq ORF: 1482 bp
• Locus ID: 18624
• UniProt ID: Q11136
• Cytogenetics: 7 20.7 cM
• Gene Summary: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because of the high level of iminoacids in collagen.[UniProtKB/Swiss-Prot Function]