Prei4 (BC033408) Mouse Tagged ORF Clone

CAT#: MR207860

Prei4 (Myc-DDK-tagged) - Mouse preimplantation protein 4 (cDNA clone MGC:38711 IMAGE:5357683)

Product Images

Circular map for MR207860

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prei4
• Synonyms: 2310004G06Rik, mKIAA1434
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207860 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAACAGCCTGGAGATATCCTTAATAAGTGACAATGAGTTCAAGTGCAGGCACTCACAGCCAGAAT
  GTGGGTATGGCTTACAGCCCGATCGTTGGACAGAGTACAGCATACAGACAATGGAACCAGATAATCTGGA
  GCTCATCTTTGACTTTTTTGAGGAAGATCTCAGTGAGCATGTAGTTCAGGGTGATGTTCTTCCTGGACAC
  GTGGGCACAGCATGCCTCCTGTCTTCTACCATTGCTGAGAGTGGAAGAAGCGCTGGAATCCTTACTCTTC
  CCATCATGAGCAGAAATTCCAGAAAAACTATAGGCAAAGTCAGAGTTGATTTTATCATCATCAAGCCATT
  ACCTGGATATAGTTGTTCTATGCAGTCTTCATTTTCCAAGTATTGGAAACCAAGAATACCATTGGACGTT
  GGACATCGTGGTGCAGGGAACTCAACAACGACTGCCAAGCTAGCTAAAGTACAGGAAAATACTATCGCTT
  CTTTAAGAAATGCTGCCAGTCATGGCGCAGCATTTGTAGAATTTGATGTCCACCTTTCAAAGGACTTTGT
  GCCCGTGGTGTATCATGACCTCACCTGCTGTCTGACCATGAAGAGGAAATATGAAGCTGATCCAGTTGAA
  TTGTTTGAAATCCCAGTAAAAGAATTAACATTTGACCAACTCCAGTTATTGAAGCTTTCTCATGTGACTG
  CATTAAAAACCAAAGACCGGAAACAATCTTTGTATGAGGAGGAAAATTTCTTTTCTGAAAATCAGCCATT
  TCCTTCTCTTAAGATGGTTTTAGAATCATTGCCAGAAAATGTAGGATTTAATATAGAAATAAAATGGATT
  TGCCAACACAGGGATGGAGTATGGGATGGCAACTTATCAACATATTTTGATATGAATGTGTTTTTGGATA
  TAATTTTAAAAACTGTTTTAGAAAATTCTGGGAAGAGGAGAATAGTGTTTTCTTCTTTTGATGCAGATAT
  TTGTACAATGGTTCGGCAGAAGCAGAACAAATATCCCATATTATTTTTGACCCAAGGAAAGTCTGATATT
  TACCCCGAACTCATGGACCTCAGATCTCGGACAACACCCATTGCAATGAGTTTTGCACAGTTTGAAAATA
  TTTTGGGGATAAATGCCCATACTGAAGACCTCCTTAGAAACCCATCCTATGTCCAAGAGGCAAAAGCTAA
  GGGATTGGTCATATTCTGCTGGGGTGATGATACCAACGATCCTGAAAACAGAAGGAAACTGAAGGAATTT
  GGAGTAAATGGTCTAATATATGATAGGATATATGATTGGATGCCTGAACAGCCAAATATATTCCAAGTGG
  AGCAGTTGGAGCGCCTGAAGCAAGAATTGCCAGAGCTTAAGAACTGTTTGTGTCCCACTGTTAGCCACTT
  CATCCCTTCTTCTTTCTGTGTGGAGCCTGATATCCACGTGGATGCCAACGGCATTGATAGTGTGGAGAAC
  GCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207860 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSNSLEISLISDNEFKCRHSQPECGYGLQPDRWTEYSIQTMEPDNLELIFDFFEEDLSEHVVQGDVLPGH
  VGTACLLSSTIAESGRSAGILTLPIMSRNSRKTIGKVRVDFIIIKPLPGYSCSMQSSFSKYWKPRIPLDV
  GHRGAGNSTTTAKLAKVQENTIASLRNAASHGAAFVEFDVHLSKDFVPVVYHDLTCCLTMKRKYEADPVE
  LFEIPVKELTFDQLQLLKLSHVTALKTKDRKQSLYEEENFFSENQPFPSLKMVLESLPENVGFNIEIKWI
  CQHRDGVWDGNLSTYFDMNVFLDIILKTVLENSGKRRIVFSSFDADICTMVRQKQNKYPILFLTQGKSDI
  YPELMDLRSRTTPIAMSFAQFENILGINAHTEDLLRNPSYVQEAKAKGLVIFCWGDDTNDPENRRKLKEF
  GVNGLIYDRIYDWMPEQPNIFQVEQLERLKQELPELKNCLCPTVSHFIPSSFCVEPDIHVDANGIDSVEN
  A
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC033408
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC033408, AAH33408
• RefSeq Size: 3174 bp
• RefSeq ORF: 1475 bp
• Locus ID: 74182
• Cytogenetics: 2
• MW: 55.9 kDa
• Gene Summary: May be involved in the negative regulation of skeletal muscle differentiation, independently of its glycerophosphocholine phosphodiesterase activity.[UniProtKB/Swiss-Prot Function]