Nle1 (NM_145431) Mouse Tagged ORF Clone

CAT#: MR207771

Nle1 (Myc-DDK-tagged) - Mouse notchless homolog 1 (Drosophila) (Nle1)

Product Images

Circular map for MR207771

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nle1
• Synonyms: AL022765; BC018399; l11Jus1; l11Jus4; Nle
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207771 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGTGGTGGAAGAGGCGGCGGCAGGCGACGTGCAGCGGCTGCTCGTACAGTTCCAGGATG
  AGGGCGGTCAGCTGCTGGGCTCCCCGTTCGACGTGCCCGTGGACATCACGCCGGACAAGCTGCAGCTGGT
  GTGCAACGCGCTGCTGGCCCAGGAGGAGCCCCTGCCCCTGGCGTTCTATGTCCACGATGCTGAGATTGTG
  TCCTCCCTGGGGAAGACGCTGGAGTCCCAGTCTGTGGAGACAGAGAAAATCGTCGATATCATCTACCAGC
  CCCAAGCTGTCTTCAGGGTCCGGGCTGTGACGCGGTGCACCAGCTCATTGGAGGGTCACAGCGAAGCTGT
  CATTTCTGTGGCCTTCAGCCCCACAGGAAAGTACCTGGCCAGTGGTTCCGGAGACACCACTGTGCGCTTC
  TGGGATCTCAGCACTGAGACACCGCACTTCACATGCAAAGGACACCGGCACTGGGTCCTTAGCATATCCT
  GGTCCCCAGATGGCAAGAAACTGGCCTCAGGCTGCAAGAATGGCCAGATTCTGCTGTGGGACCCAAGCAC
  GGGGCTGCAGGTGGGCAGGACCCTCACTGGCCACAGCAAATGGATCACAGGCCTGAGCTGGGAGCCCCTT
  CACATGAACCCCGAGTGCCGCTACGTGGCCAGCAGCTCCAAAGACGGCAGTGTACGAGTCTGGGATACAA
  CCGCAGGCCGCTGTGAACGCATCCTCACGGGACACACGCAGTCAGTCACCTGCCTCCGATGGGGAGGAGA
  TGGGCTTCTATATTCTGCTTCCCAGGACCGAACCATCAAAGTCTGGAGGGCCCATGACGGTGTGCTGTGT
  CGTACTCTTCAAGGTCATGGCCACTGGGTGAACACCATGGCGCTTAGCACAGACTATGCCCTGCGCACAG
  GGGCCTTTGAGCCTGCTGAAGCCACAGTGAATGCCCAAGACCTGCAGGGGTCCTTGAAGGAGCTGAAGGA
  GAGGGCATCGAGCCGCTACAACCTCGTGCGGGGCCAAGGCCCAGAGAGGCTGGTCTCTGGCTCAGACGAC
  TTCACCTTATTCCTGTGGTCCCCAGCAGAGGACAAGAAGCCCCTCGCCCGGATGACAGGACACCAGGCCC
  TCATCAACCAGGTGCTCTTCTCTCCTGACTCGCGCATCGTGGCCAGTGCCTCCTTCGACAAGTCTATCAA
  GCTGTGGGATGGCCGGACGGGCAAGTACCTAGCTTCCCTGCGTGGCCACGTGGCAGCCGTGTACCAGATT
  GCCTGGTCAGCTGACAGCCGACTATTAGTCAGCGGCAGCAGTGACAGCACGCTGAAGGTGTGGGATGTGA
  AGGCCCAGAAACTGGCCACGGACCTGCCTGGCCATGCCGATGAGGTATATGCTGTTGACTGGAGTCCAGA
  TGGCCAGAGAGTAGCAAGCGGCGGGAAAGACAAGTGCCTCCGGATATGGAGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207771 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVVEEAAAGDVQRLLVQFQDEGGQLLGSPFDVPVDITPDKLQLVCNALLAQEEPLPLAFYVHDAEIV
  SSLGKTLESQSVETEKIVDIIYQPQAVFRVRAVTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRF
  WDLSTETPHFTCKGHRHWVLSISWSPDGKKLASGCKNGQILLWDPSTGLQVGRTLTGHSKWITGLSWEPL
  HMNPECRYVASSSKDGSVRVWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHDGVLC
  RTLQGHGHWVNTMALSTDYALRTGAFEPAEATVNAQDLQGSLKELKERASSRYNLVRGQGPERLVSGSDD
  FTLFLWSPAEDKKPLARMTGHQALINQVLFSPDSRIVASASFDKSIKLWDGRTGKYLASLRGHVAAVYQI
  AWSADSRLLVSGSSDSTLKVWDVKAQKLATDLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145431
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145431.1, NP_663406.1
• RefSeq Size: 1771 bp
• RefSeq ORF: 1458 bp
• Locus ID: 217011
• UniProt ID: Q8VEJ4
• Cytogenetics: 11 C
• MW: 53.1 kDa
• Gene Summary: Plays a role in regulating Notch activity (Probable). Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival.[UniProtKB/Swiss-Prot Function]