Sf3a2 (NM_013651) Mouse Tagged ORF Clone

CAT#: MR207770

Sf3a2 (Myc-DDK-tagged) - Mouse splicing factor 3a, subunit 2 (Sf3a2)

Product Images

Circular map for MR207770

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sf3a2
• Synonyms: 66kDa; AW047242; PRP11; Sap62; SFA66
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207770 representing NM_013651
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTTTCAGCACAGACCTGGAGGCAAGACTGGGAGTGGGGGCGTAGCCTCCTCCTCTGAGAGCAACC
  GGGATCGGAGAGAGCGTCTGCGCCAATTGGCCCTGGAAACCATTGACATCAACAAGGACCCGTATTTTAT
  GAAGAACCACTTGGGATCCTATGAGTGCAAACTCTGCCTGACTCTGCATAACAATGAGGGGAGCTACCTG
  GCCCATACCCAAGGGAAGAAACATCAGACTAACTTGGCCCGGCGAGCTGCCAAGGAGGCCAAAGAAGCCC
  CCGCCCAGCCTGCACCGGAGAAGGTCAAGGTGGAGGTGAAGAAGTTTGTGAAGATTGGCCGCCCTGGGTA
  CAAAGTGACCAAGCAGAGGGACACAGAGATGGGCCAGCAGAGCCTGCTGTTCCAGATTGACTACCCTGAG
  ATCGCAGAGGGTATCATGCCACGCCACCGTTTCATGTCTGCTTATGAGCAGAGGATCGAGCCTCCAGACC
  GCCGCTGGCAGTACCTGCTCATGGCTGCTGAGCCCTATGAGACCATTGCTTTCAAGGTGCCAAGCCGGGA
  GATTGACAAGGCTGAGGGCAAGTTTTGGACCCACTGGAACAGAGAAACCAAGCAGTTTTTTCTTCAGTTC
  CACTTTAAGATGGAGAAGCCCCCAGCACCTCCCAGCCTCCCAGCAGGACCCCCAGGCGTCAAGCGGCCCC
  CGCCCCCACTCATGAATGGACTGCCTCCTCGCCCACCACTCCCTGATGCCTTGCCCCCACCTCCACCTGG
  TGGCCTGCCTCTCCCTCCTATGCCCCCCACTGGGCCTGCTCCCTCTGGGCCCCCTGGACCTCCCCAGATG
  CCCCCACCAGCTCCTGGGGTGCACCCCCCGGCCCCAGTAGTACACCCACCAACATCTGGAGTCCATCCCC
  CAGCTCCTGGGGTACACCCCCCAGCACCAGTGGTCCATCCACCAACATCTGGGGTCCATCCCCCAGCCCC
  TGGAGTGCACCCACCCACCCCTGGAGTGCACCCACCAGCCCCAGGAGTGCACCCACCAGCTCCAGGTGTC
  CACCCACCAGCTCCAGGGGTACACCCCCCAACTCCAGGGGTGCACCCACCAGCTCCAGGGGTGCACCCAC
  CAGCTCCAGGGGTGCACCCACCAGCTCCAGGGGTACACCCTCCTCCATCAGCTGGAGTTCATCCTCAGGC
  TCCAGGGGTACACCCACCTGCTCCTGCAGTACACCCTCAAGCCCCTGGGGTACACCCCCCAGCCCCTGGG
  ATCCACCCTCAGGCACCAGGGGTTCACCCTCAGCCGCCTCCTGGAGTCCACCCTGCTGCCCCGGGGGTCC
  ACCCTCAGCCTCCAGGGGTTCACCCCTCAAATCCTGGGGTGCACCCTGCTCCTATGCCCCCCATGCTGAG
  GCCCCCACTCCCCTCAGATGGCCCTGGGAACATGCCTCCCCCTCCCCCAGGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207770 representing NM_013651
  Red=Cloning site Green=Tags(s)
  
  MDFQHRPGGKTGSGGVASSSESNRDRRERLRQLALETIDINKDPYFMKNHLGSYECKLCLTLHNNEGSYL
  AHTQGKKHQTNLARRAAKEAKEAPAQPAPEKVKVEVKKFVKIGRPGYKVTKQRDTEMGQQSLLFQIDYPE
  IAEGIMPRHRFMSAYEQRIEPPDRRWQYLLMAAEPYETIAFKVPSREIDKAEGKFWTHWNRETKQFFLQF
  HFKMEKPPAPPSLPAGPPGVKRPPPPLMNGLPPRPPLPDALPPPPPGGLPLPPMPPTGPAPSGPPGPPQM
  PPPAPGVHPPAPVVHPPTSGVHPPAPGVHPPAPVVHPPTSGVHPPAPGVHPPTPGVHPPAPGVHPPAPGV
  HPPAPGVHPPTPGVHPPAPGVHPPAPGVHPPAPGVHPPPSAGVHPQAPGVHPPAPAVHPQAPGVHPPAPG
  IHPQAPGVHPQPPPGVHPAAPGVHPQPPGVHPSNPGVHPAPMPPMLRPPLPSDGPGNMPPPPPGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013651
• ORF Size: 1455 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013651.4, NP_038679.3
• RefSeq Size: 1674 bp
• RefSeq ORF: 1458 bp
• Locus ID: 20222
• Cytogenetics: 10 39.72 cM
• MW: 51.3 kDa