Gdf10 (NM_145741) Mouse Tagged ORF Clone

CAT#: MR207626

Gdf10 (Myc-DDK-tagged) - Mouse growth differentiation factor 10 (Gdf10)

Product Images

Circular map for MR207626

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gdf10
• Synonyms: Bmp3; Bmp3b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207626 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCAGGTCCTGCTCGGATCAGCTTGGGGTCCCAGCTGCTGCCCATGGTGCCGCTGCTCCTGCTGC
  TGCGGGGCGCAGGCTGCGGCCACAGGGGCCCCTCATGGTCCTCATTGCCCTCGGCAGCTGCCGGTCTGCA
  GGGGGACAGGGACTCCCAGCAGTCACCCGGGGACGCAGCAGCCGCTCTGGGCCCAGGCGCCCAGGACATG
  GTCGCTATCCACATGCTCAGGCTCTATGAGAAGTACAACCGAAGAGGTGCTCCACCGGGAGGAGGCAACA
  CCGTCCGAAGCTTCCGTGCCCGGCTGGAAATGATCGACCAAAAGCCTGTGTATTTCTTCAACTTGACTTC
  CATGCAAGACTCAGAAATGATCCTCACAGCCGCCTTCCACTTCTACTCAGAACCTCCACGGTGGCCCCGG
  GCTCGTGAGGTATTCTGCAAGCCCCGAGCTAAGAACGCATCCTGCCGCCTCCTGACCCCAGGGCTGCCTG
  CACGCTTGCACCTAATCTTCCGCAGTCTTTCCCAGAACACCGCCACTCAGGGGCTGCTCCGCGGGGCCAT
  GGCCCTGACGCCTCCACCACGTGGCCTGTGGCAGGCCAAGGACATCTCCTCAATCATCAAGGCTGCCCGA
  AGGGATGGAGAGCTGCTTCTCTCTGCTCAGCTGGATACTGGGGAGAAGGACCCCGGAGTGCCACGGCCCA
  GTTCCCACATGCCCTATATCCTTGTCTACGCCAATGACCTGGCCATCTCCGAACCCAACAGTGTAGCAGT
  GTCGCTACAGAGATACGACCCATTTCCAGCTGGAGACTTTGAGCCTGGAGCAGCCCCCAACAGCTCAGCT
  GATCCCCGCGTGCGCAGGGCGGCTCAGGTGTCAAAACCCCTGCAAGACAATGAACTGCCGGGGCTGGATG
  AAAGACCAGCGCCTGCCCTGCATGCCCAGAATTTCCACAAGCACGAGTTCTGGTCCAGTCCTTTCCGGGC
  ACTGAAACCCCGCACGGGGCGCAAAGACCGCAAGAAGAAGGACCAGGACACATTCACCGCCGCCTCCTCT
  CAGGTGCTGGACTTTGACGAGAAGACGATGCAGAAAGCCAGGAGGCGGCAGTGGGATGAGCCCCGGGTCT
  GCTCCAGGAGGTACCTGAAGGTGGATTTTGCAGACATCGGGTGGAATGAATGGATCATCTCTCCCAAATC
  CTTTGACGCCTACTACTGTGCTGGGGCCTGCGAGTTCCCCATGCCCAAGATTGTCCGCCCATCCAACCAT
  GCCACCATCCAGAGCATCGTCAGAGCTGTGGGCATTGTCCCTGGCATCCCAGAGCCATGCTGTGTTCCAG
  ACAAGATGAACTCCCTTGGAGTCCTTTTCCTGGATGAAAATCGGAATGCGGTTCTGAAGGTGTACCCCAA
  TATGTCCGTAGAGACCTGTGCCTGTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207626 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPGPARISLGSQLLPMVPLLLLLRGAGCGHRGPSWSSLPSAAAGLQGDRDSQQSPGDAAAALGPGAQDM
  VAIHMLRLYEKYNRRGAPPGGGNTVRSFRARLEMIDQKPVYFFNLTSMQDSEMILTAAFHFYSEPPRWPR
  AREVFCKPRAKNASCRLLTPGLPARLHLIFRSLSQNTATQGLLRGAMALTPPPRGLWQAKDISSIIKAAR
  RDGELLLSAQLDTGEKDPGVPRPSSHMPYILVYANDLAISEPNSVAVSLQRYDPFPAGDFEPGAAPNSSA
  DPRVRRAAQVSKPLQDNELPGLDERPAPALHAQNFHKHEFWSSPFRALKPRTGRKDRKKKDQDTFTAASS
  QVLDFDEKTMQKARRRQWDEPRVCSRRYLKVDFADIGWNEWIISPKSFDAYYCAGACEFPMPKIVRPSNH
  ATIQSIVRAVGIVPGIPEPCCVPDKMNSLGVLFLDENRNAVLKVYPNMSVETCACR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145741
• ORF Size: 1431 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145741.3
• RefSeq Size: 2613 bp
• RefSeq ORF: 1431 bp
• Locus ID: 14560
• UniProt ID: P97737
• Cytogenetics: 14 20.8 cM
• MW: 52.6 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein has been shown to promote neural repair after stroke and may act as a tumor suppressor. [provided by RefSeq, Jul 2016]