Cndp2 (NM_023149) Mouse Tagged ORF Clone

CAT#: MR207616

Cndp2 (Myc-DDK-tagged) - Mouse CNDP dipeptidase 2 (metallopeptidase M20 family) (Cndp2)

Product Images

Circular map for MR207616

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cndp2
• Synonyms: 0610010E05Rik; C76600; Cn2; Dip-2; Pep-1; Pep1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207616 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCCCTCAAAGCTGTCTTCCAGTATATCGATGAAAACCAGGACCGCTACGTCAAGAAACTTGCAG
  AATGGGTGGCCATCCAGAGCGTGTCCGCATGGCCGGAGAAGAGAGGAGAGATCAGAAGGATGATGGAGGT
  GGCAGCTGCCGATGTCCAGAGGCTGGGGGGCTCCGTGGAACTGGTGGATATTGGGAAGCAGAAGCTCCCA
  GATGGCTCGGAGATACCACTTCCTCCCATTCTGCTGGGCAAGCTAGGCAGCGACCCCCAGAAGAAAACCG
  TGTGCATTTACGGGCACCTGGACGTGCAGCCTGCGGCCCTGGAGGACGGGTGGGACAGCGAGCCCTTCAC
  CTTGGTGGAGCGGGAAGGCAAGCTGTATGGGAGAGGCTCCACGGACGATAAGGGGCCAGTGGCCGGGTGG
  ATGAATGCCCTGGAAGCCTATCAGAAGACTGGTCAGGAGATCCCAGTCAACCTGCGGTTCTGCCTGGAAG
  GCATGGAGGAGTCTGGCTCCGAAGGCCTGGATGAGCTCATTTTTGCCCAGAAAGACAAGTTCTTCAAAGA
  TGTGGACTATGTGTGCATTTCAGACAATTACTGGCTGGGGAAGAATAAGCCGTGTATCACGTACGGCCTC
  AGAGGCATCTGTTACTTCTTCATCGAGGTGGAATGCAGTGACAAAGACCTCCATTCTGGGGTATATGGAG
  GCTCAGTGCACGAGGCCATGACGGATCTCATTTCACTGATGGGCTGCCTGGTAGACAAGAAGGGGAAGAT
  CCTCATCCCGGGCATCAACGATGCCGTGGCGCCTGTGACGGATGAGGAGCATGCGCTCTACGACCACATT
  GACTTCGATATGGAAGAGTTCGCCAAGGACGTGGGGGCTGAGACCCTTCTGCACAGCTGCAAGAAAGACA
  TCCTGATGCACAGATGGCGATACCCGTCCCTCTCCCTGCATGGGATCGAAGGGGCCTTCTCCGGTTCAGG
  GGCCAAGACTGTGATCCCCAGAAAGGTGGTCGGCAAGTTCTCCATCAGGCTCGTGCCAGACATGATACCG
  GAAGTTGTTAGCGAGCAAGTTTCAAGCTACTTGTCAAAAAAATTTGCTGAACTGCAGAGCCCCAACAAGT
  TCAAGGTGTACATGGGCCACGGCGGGAAGCCCTGGGTGTCTGACTTCAACCATCCTCATTACCAGGCGGG
  GAGAAGAGCCCTGAAGACAGTGTTTGGTGTTGAGCCTGACTTGACCAGAGAAGGTGGCAGTATTCCTGTG
  ACCTTGACCTTTCAAGAGGCCACAGGCAAAAATGTCATGCTATTGCCAGTGGGGTCAGCAGATGACGGTG
  CCCACTCGCAGAATGAGAAGCTCAACAGGCTCAACTACATAGAAGGAACCAAAATGCTGGCTGCATATCT
  GTATGAGGTGTCTCAGCTGAAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207616 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSALKAVFQYIDENQDRYVKKLAEWVAIQSVSAWPEKRGEIRRMMEVAAADVQRLGGSVELVDIGKQKLP
  DGSEIPLPPILLGKLGSDPQKKTVCIYGHLDVQPAALEDGWDSEPFTLVEREGKLYGRGSTDDKGPVAGW
  MNALEAYQKTGQEIPVNLRFCLEGMEESGSEGLDELIFAQKDKFFKDVDYVCISDNYWLGKNKPCITYGL
  RGICYFFIEVECSDKDLHSGVYGGSVHEAMTDLISLMGCLVDKKGKILIPGINDAVAPVTDEEHALYDHI
  DFDMEEFAKDVGAETLLHSCKKDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPRKVVGKFSIRLVPDMIP
  EVVSEQVSSYLSKKFAELQSPNKFKVYMGHGGKPWVSDFNHPHYQAGRRALKTVFGVEPDLTREGGSIPV
  TLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRLNYIEGTKMLAAYLYEVSQLKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023149
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023149.3
• RefSeq Size: 2137 bp
• RefSeq ORF: 1428 bp
• Locus ID: 66054
• UniProt ID: Q9D1A2
• Cytogenetics: 18 E4
• MW: 52.8 kDa
• Gene Summary: Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Catalyzes the production of N-lactoyl-amino acids from lactate and amino acids by reverse proteolysis.[UniProtKB/Swiss-Prot Function]