Rufy3 (NM_027530) Mouse Tagged ORF Clone

CAT#: MR207512

Rufy3 (Myc-DDK-tagged) - Mouse RUN and FYVE domain containing 3 (Rufy3)

Product Images

Circular map for MR207512

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rufy3
• Synonyms: 2810428M05Rik; 6330416M07Rik; AW455998; AW538594; D5Bwg0860e; Ripx; Rpipx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207512 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCCCTGACGCCTCCGACTGATATGCCAACCCCCACCACTGACAAGATCACACAGGCTGCCATGG
  AGACCATCTACCTTTGCAAATTCCGAGTGTCTATGGACGGAGAATGGCTCTGCCTTCGGGAGCTGGATGA
  CATCTCCCTTACACCCGACCCAGAGCCGACCCATGAAGATCCCAATTATCTCATGGCTAACGAACGCATG
  AACCTGATGAACATGGCAAAGCTGAGCATCAAGGGCTTGATTGAATCGGCTCTGAATCTGGGGCGGACCC
  TGGACTCTGACTACGCACCTCTCCAGCAGTTTTTCGTGGTGATGGAACACTGCCTGAAACACGGCTTGAA
  AGCCAAGAAAACTTTTCTTGGACAAAATAAATCCTTCTGGGGGCCTCTAGAGCTGGTGGAGAAGCTTGTT
  CCAGAAGCTGCAGAAATAACAGCGAGTGTAAAAGACCTCCCAGGACTCAAGACACCAGTTGGCAGAGGAA
  GAGCCTGGCTTCGGTTGGCATTGATGCAAAAGAAGCTTTCTGAGTATATGAAAGCCTTGATCAATAAGAA
  GGAACTTCTCAGTGAGTTCTATGAAGTCAATGCCCTCATGATGGAAGAAGAAGGAGCTATTATCGCTGGT
  CTCCTGGTGGGTCTGAATGTCATCGATGCCAATTTCTGCATAAAAGGAGAAGACCTGGACTCTCAGGTTG
  GAGTTATAGATTTTTCGATGTATCTCAAAGATGGAAACAGTAGTAAAGGTAGTGAAGGGGATGGACAGAT
  TACTGCGATCCTAGACCAGAAGAACTATGTAGAAGAACTCAACAGACATCTGAATGCAACTGTAAACAAC
  CTTCAGACAAAAGTAGATCTGTTAGAAAAATCCAACACTAAATTGACAGAAGAACTTGCCGTTGCCAACA
  ACAGAATTATTACCTTACAAGAAGAAATGGAACGAGTTAAAGAAGAAAGCTCCTATCTACTGGAATCCAA
  TCGGAAGGGTCCTAAACAAGACAGAACTGCAGAAGGGCAAGCGCTGAGCGAAGCCAGAAAGCATCTAAAG
  GAGGAGACACAGTTAAGATTGGATGTCGAGAAGGAGCTGGAGCTGCAGATCAGCATGAGGCAGGAGATGG
  AACTGGCTATGAAGATGCTGGAGAAGGATGTCTGTGAGAAGCAGGATGCCCTGGTGTCTCTGCGGCAGCA
  GCTGGACGATCTCCGGGCTCTTAAGCATGAGCTCGCCTTTAAACTGCAGAGTTCAGACCTAGGAGTGAAA
  CAGAAAAGTGAATTAAACAGTCGCTTGGAAGAAAAGACCAATCAGATGGCTGCCACCATTAAACAGCTGG
  AGCAAAGTGAAAAAGATTTGGTGAAACAGGCAAAGACCTTAAATAGTGCAGCAAATAAACTGATCCCAAA
  GCACCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207512 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSALTPPTDMPTPTTDKITQAAMETIYLCKFRVSMDGEWLCLRELDDISLTPDPEPTHEDPNYLMANERM
  NLMNMAKLSIKGLIESALNLGRTLDSDYAPLQQFFVVMEHCLKHGLKAKKTFLGQNKSFWGPLELVEKLV
  PEAAEITASVKDLPGLKTPVGRGRAWLRLALMQKKLSEYMKALINKKELLSEFYEVNALMMEEEGAIIAG
  LLVGLNVIDANFCIKGEDLDSQVGVIDFSMYLKDGNSSKGSEGDGQITAILDQKNYVEELNRHLNATVNN
  LQTKVDLLEKSNTKLTEELAVANNRIITLQEEMERVKEESSYLLESNRKGPKQDRTAEGQALSEARKHLK
  EETQLRLDVEKELELQISMRQEMELAMKMLEKDVCEKQDALVSLRQQLDDLRALKHELAFKLQSSDLGVK
  QKSELNSRLEEKTNQMAATIKQLEQSEKDLVKQAKTLNSAANKLIPKHH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027530
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027530.1, NM_027530.2, NM_027530.3, NP_081806.1
• RefSeq Size: 4097 bp
• RefSeq ORF: 1410 bp
• Locus ID: 52822
• UniProt ID: Q9D394
• Cytogenetics: 5 43.77 cM
• MW: 53 kDa
• Gene Summary: Plays a role in the generation of neuronal polarity formation and axon growth (PubMed:24720729). Implicated in the formation of a single axon by developing neurons (PubMed:24720729). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery (By similarity). Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (PubMed:24720729). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]