Rnmt (NM_026440) Mouse Tagged ORF Clone

CAT#: MR207439

Rnmt (Myc-DDK-tagged) - Mouse RNA (guanine-7-) methyltransferase (Rnmt), transcript variant 1

Product Images

Circular map for MR207439

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnmt
• Synonyms: 2610002P10Rik; AI848273; mKIAA0398; Rg7mt1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207439 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGTTCCGCGAAAGCGTCAGTCGCTTCTGACCCAGAGTCCCCTCCAGGTGGTAATGAGCCCGCTG
  CTGCCTCTGGGCAGAGGCTTCCAGAAAATACTCCTCCATGCCAGCAAGTAGATCAGCCAAAAATGCAGAA
  GGAGTTTGGGGAGGATCTTGTAGAGCAAAATTCGAGTTATGTGCAAGATTCTCCATCCAAGAAAAGGAAA
  CTTGATGTTGAAATCATCTTAGAGGAAAAGCACTCTGAGGATGATGGAGGTTCGGCAAAGAGAAGCAAAC
  TGGAAAGAGGTGATGTGTCGGAAGATGAGCCTTCCCTTGGTAGGCTTAACCAGACCAAGAGAAAACTGCA
  GCCTCAGGACGATGAGGTACCGCAGAAGTTGCAGAAGCTGGAGGAAGGACACAGCTCAGCCGTGGCTGCC
  CACTATAATGAGCTTCAGGAAGTTGGCCTGGCGAAGCGTAGTCAAAGTCGCATTTTTTACCTAAGAAACT
  TCAATAATTGGATTAAAAGTATTCTGATTGGGGAAATTTTAGAAAAGGTACGACAAAGAAAAACCCGTGA
  CATCACTGTTTTGGACCTGGGATGTGGAAAAGGTGGAGATTTGCTCAAGTGGAGGAAGGGGAGAATTAGC
  AGGCTAGTGTGTGCTGATATCGCTGATATTTCTATGAAACAATGTCAACAGCGTTACGAAGACATGAGAT
  GTCGTCGCGATAATGAGCATATTTTTAGTGCAGAATTTATAACTGCTGACTGTTCAAAGGAACTTTTGGT
  TGAAAAATTCCGTGACCCAGAAATGTACTTTGATGTCTGCAGCTGCCAGTTTGCTTGCCATTACTCCTTT
  GAGTCCCAGGTGCAGGCGGATACGATGCTTAGAAATGCCTGCGGGAGGCTTAACCCTGGCGGCTATTTTA
  TCGGCACCACTCCCAATAGCTTTGAACTGATAAGACGCCTTGAAGCTTCAGAAACAGAATCATTTGGAAA
  TGAAATATACACTGTGAAATTCCAGAAGAAAGGAAATTATCCTTTATTTGGCTGCAAATATGACTTTAAC
  TTGGAAGGTGTTGTGGATGTCCCTGAATTCTTGGTCTATTTTCCATTGCTAACTGAAATGGCAAAGAAGT
  ATAATATGAAACTAATCTACAAAAAAACATTTTTGGAATTCTATGAAGAAAAGATTAAAAACAATGAAAA
  CAAAATGCTCTTAAAACGAATGCAGGCCCTGGAGCAATACCCTGCACATGAGAACTCTAAACTTGCCTCT
  GAAAAGGTGGGTGACTACACGCATGCAGCAGAGTACCTGAAGAAGAGCCAAGTGAGGTTACCTCTGGGAA
  CCTTAAGTAAGTCAGAATGGGAAGCTACAAGTATCTACTTGGTCTTTGCCTTTGAGAAGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207439 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGSAKASVASDPESPPGGNEPAAASGQRLPENTPPCQQVDQPKMQKEFGEDLVEQNSSYVQDSPSKKRK
  LDVEIILEEKHSEDDGGSAKRSKLERGDVSEDEPSLGRLNQTKRKLQPQDDEVPQKLQKLEEGHSSAVAA
  HYNELQEVGLAKRSQSRIFYLRNFNNWIKSILIGEILEKVRQRKTRDITVLDLGCGKGGDLLKWRKGRIS
  RLVCADIADISMKQCQQRYEDMRCRRDNEHIFSAEFITADCSKELLVEKFRDPEMYFDVCSCQFACHYSF
  ESQVQADTMLRNACGRLNPGGYFIGTTPNSFELIRRLEASETESFGNEIYTVKFQKKGNYPLFGCKYDFN
  LEGVVDVPEFLVYFPLLTEMAKKYNMKLIYKKTFLEFYEEKIKNNENKMLLKRMQALEQYPAHENSKLAS
  EKVGDYTHAAEYLKKSQVRLPLGTLSKSEWEATSIYLVFAFEKQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026440
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026440.1, NM_026440.2, NM_026440.3, NM_026440.4, NP_080716.1
• RefSeq Size: 5018 bp
• RefSeq ORF: 1398 bp
• Locus ID: 67897
• UniProt ID: Q9D0L8
• Cytogenetics: 18 E2
• MW: 53.3 kDa
• Gene Summary: Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAMAC complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.[UniProtKB/Swiss-Prot Function]