Pold3 (NM_133692) Mouse Tagged ORF Clone
CAT#: MR207366
- TrueORF®
Pold3 (Myc-DDK-tagged) - Mouse polymerase (DNA-directed), delta 3, accessory subunit (Pold3)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_133692" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Pold3 |
Synonyms | 2410142G14Rik; C85233; P66; P68 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207366 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAACAGCTGTATCTAGAAAACATAGACGAGTTCGTCACGGACCAGAACAAGATCGTGACTTACA AGTGGCTAAGCTATACACTAGGGGTTCATGTTAACCAGGCCAAACAGATGCTCTATGAATATGTTGAAAG GAAACGGAAAGAAAATTCGGGAGCTCAGCTGCATGTTACCTACTTGGTGTCTGGCAGTCTTATACAGAAC GGACATTCTTGCCACAAGGTTGCAGTAGTGAGAGAAGATAAACTGGAAGCAGTGAAGTCCAAGTTAGCTG TGACTGCCAGCATCCATGTGTACAGCATCCAGAAAGCTATGCTAAAGGACAGTGGGCCTCTGTTCAATAC CGACTATGACATCCTTAAAAGCAATTTGCAGAACTGCAGCAAGTTTAGTGCCATACAGTGTGCAGCTGCA GTCCCCAGAGCTCCTGCAGAATCCCCATCTTCCAGAAAGTATGAACAGTCAAATCTTCAGGCAGCGAGTG AGGCACAAGCCAGTGAGCTGACTACCAATGGCCATGGTCCACCTGCCTCCAAACAGGCTTCCCAGCAGCC CAAAGGAATTATGGGAATGTTAATCTCTAAAGCTGCTACTAAAACCCAGGACACCAACAAGGAAACCAAA CCAGAGGCCCGAGAAGTAACCAGTGCATCTTCTGCTGGGGGCAAAGCACCAGGAAAAGGGAGTGTGATGA GCAACTTTTTTGGAAAAGCTGCAATGAATAAACTTAAAGTCAATTTGGATTCAGAACAAGCAGTAAAGGA AGAAAAAACAGTGGAGCAACCTCCAGTGTCTGTCACTGAACCAAAGCTGGCAGCTCCCCCAGCTCAGAAG AAATCCAGCAGAAAGTCCGAGCCTGGGAAGGTGCAGCAGAAGGAGAAAAGCAGGGGCAAGCGAGTAGACT TGTCGGATGAGGAGGCAAAGGAAACCGAACACCTGAAGAAAAAGAGAAGAAGAATCAAGCTTCCTCAGTC TGATAGCAGTGAAGATGAAGTCTTCGAAGACTCCCCTGAGATGTATGAAGCAGACTCACCATCTCCACCT CCTGTATCTCCACCTCCTGATCCTATGCCAAAAACTGAGCCCCCTCCTGTCAAGCGTTCAAGTGGAGAAA CCAAAAGGAGACGAAAGCGTGTACTGAAATCTAAAACCTTTGTGGATGAAGAAGGCTGCATAGTGACTGA GAAAGTCTATGAGAGTGAGTCCTGCACAGACAGTGAGGAGGAGCTTAAGATGAAGCCGGCCTCAGCACAC AAACCCCCTGCTGCCGCTGTGAAAAGGGAGCCCAGAGAAGAACGGAAGGGCCCCAAGAAAGGGGCTGCTG CTCTGGGCAAAGCCAACAGACAAGTGTCCATTACTGGCTTCTTCCAGAAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207366 protein sequence
Red=Cloning site Green=Tags(s) MAEQLYLENIDEFVTDQNKIVTYKWLSYTLGVHVNQAKQMLYEYVERKRKENSGAQLHVTYLVSGSLIQN GHSCHKVAVVREDKLEAVKSKLAVTASIHVYSIQKAMLKDSGPLFNTDYDILKSNLQNCSKFSAIQCAAA VPRAPAESPSSRKYEQSNLQAASEAQASELTTNGHGPPASKQASQQPKGIMGMLISKAATKTQDTNKETK PEAREVTSASSAGGKAPGKGSVMSNFFGKAAMNKLKVNLDSEQAVKEEKTVEQPPVSVTEPKLAAPPAQK KSSRKSEPGKVQQKEKSRGKRVDLSDEEAKETEHLKKKRRRIKLPQSDSSEDEVFEDSPEMYEADSPSPP PVSPPPDPMPKTEPPPVKRSSGETKRRRKRVLKSKTFVDEEGCIVTEKVYESESCTDSEEELKMKPASAH KPPAAAVKREPREERKGPKKGAAALGKANRQVSITGFFQKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_133692 |
ORF Size | 1386 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133692.2, NP_598453.1 |
RefSeq Size | 3002 bp |
RefSeq ORF | 1386 bp |
Locus ID | 67967 |
Cytogenetics | 7 E2 |
MW | 50.7 kDa |
Gene Summary | As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:10219083, PubMed:27524497). Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216088 | Pold3 (untagged) - Mouse polymerase (DNA-directed), delta 3, accessory subunit (Pold3), (10ug) |
USD 503.00 |
|
MG207366 | Pold3 (tGFP-tagged) - Mouse polymerase (DNA-directed), delta 3, accessory subunit (Pold3) |
USD 657.00 |
|
MR207366L3 | Lenti ORF clone of Pold3 (Myc-DDK-tagged) - Mouse polymerase (DNA-directed), delta 3, accessory subunit (Pold3) |
USD 757.00 |
|
MR207366L4 | Lenti ORF clone of Pold3 (mGFP-tagged) - Mouse polymerase (DNA-directed), delta 3, accessory subunit (Pold3) |
USD 757.00 |
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