Pold3 (NM_133692) Mouse Untagged Clone

CAT#: MC216088

Pold3 (untagged) - Mouse polymerase (DNA-directed), delta 3, accessory subunit (Pold3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pold3
• Synonyms: 2410142G14Rik; C85233; P66; P68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216088 representing NM_133692
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAACAGCTGTATCTAGAAAACATAGACGAGTTCGTCACGGACCAGAACAAGATCGTGACTTACA
  AGTGGCTAAGCTATACACTAGGGGTTCATGTTAACCAGGCCAAACAGATGCTCTATGAATATGTTGAAAG
  GAAACGGAAAGAAAATTCGGGAGCTCAGCTGCATGTTACCTACTTGGTGTCTGGCAGTCTTATACAGAAC
  GGACATTCTTGCCACAAGGTTGCAGTAGTGAGAGAAGATAAACTGGAAGCAGTGAAGTCCAAGTTAGCTG
  TGACTGCCAGCATCCATGTGTACAGCATCCAGAAAGCTATGCTAAAGGACAGTGGGCCTCTGTTCAATAC
  CGACTATGACATCCTTAAAAGCAATTTGCAGAACTGCAGCAAGTTTAGTGCCATACAGTGTGCAGCTGCA
  GTCCCCAGAGCTCCTGCAGAATCCCCATCTTCCAGAAAGTATGAACAGTCAAATCTTCAGGCAGCGAGTG
  AGGCACAAGCCAGTGAGCTGACTACCAATGGCCATGGTCCACCTGCCTCCAAACAGGCTTCCCAGCAGCC
  CAAAGGAATTATGGGAATGTTAATCTCTAAAGCTGCTACTAAAACCCAGGACACCAACAAGGAAACCAAA
  CCAGAGGCCCGAGAAGTAACCAGTGCATCTTCTGCTGGGGGCAAAGCACCAGGAAAAGGGAGTGTGATGA
  GCAACTTTTTTGGAAAAGCTGCAATGAATAAACTTAAAGTCAATTTGGATTCAGAACAAGCAGTAAAGGA
  AGAAAAAACAGTGGAGCAACCTCCAGTGTCTGTCACTGAACCAAAGCTGGCAGCTCCCCCAGCTCAGAAG
  AAATCCAGCAGAAAGTCCGAGCCTGGGAAGGTGCAGCAGAAGGAGAAAAGCAGGGGCAAGCGAGTAGACT
  TGTCGGATGAGGAGGCAAAGGAAACCGAACACCTGAAGAAAAAGAGAAGAAGAATCAAGCTTCCTCAGTC
  TGATAGCAGTGAAGATGAAGTCTTCGAAGACTCCCCTGAGATGTATGAAGCAGACTCACCATCTCCACCT
  CCTGTATCTCCACCTCCTGATCCTATGCCAAAAACTGAGCCCCCTCCTGTCAAGCGTTCAAGTGGAGAAA
  CCAAAAGGAGACGAAAGCGTGTACTGAAATCTAAAACCTTTGTGGATGAAGAAGGCTGCATAGTGACTGA
  GAAAGTCTATGAGAGTGAGTCCTGCACAGACAGTGAGGAGGAGCTTAAGATGAAGCCGGCCTCAGCACAC
  AAACCCCCTGCTGCCGCTGTGAAAAGGGAGCCCAGAGAAGAACGGAAGGGCCCCAAGAAAGGGGCTGCTG
  CTCTGGGCAAAGCCAACAGACAAGTGTCCATTACTGGCTTCTTCCAGAAAAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133692
• Insert Size: 1386 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133692.2, NP_598453.1
• RefSeq Size: 3002 bp
• RefSeq ORF: 1386 bp
• Locus ID: 67967
• Cytogenetics: 7 E2
• Gene Summary: As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair (PubMed:10219083, PubMed:27524497). Required for optimal Pol-delta activity. Stabilizes the Pol-delta complex and plays a major role in Pol-delta stimulation by PCNA. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may also be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. In this context, POLD3, along with PCNA and RFC1-replication factor C complex, is required to recruit POLD1, the catalytic subunit of the polymerase delta complex, to DNA damage sites. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites performed by Pol-delta4, independently of DNA polymerase zeta (REV3L) or eta (POLH). Facilitates abasic site bypass by DNA polymerase delta by promoting extension from the nucleotide inserted opposite the lesion. Also involved in TLS, as a component of the POLZ complex. Along with POLD2, dramatically increases the efficiency and processivity of DNA synthesis of the minimal DNA polymerase zeta complex, consisting of only REV3L and REV7 (By similarity).[UniProtKB/Swiss-Prot Function]