Nucb1 (NM_001163662) Mouse Tagged ORF Clone

CAT#: MR207335

Nucb1 (Myc-DDK-tagged) - Mouse nucleobindin 1 (Nucb1), transcript variant 1

Product Images

Circular map for MR207335

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nucb1
• Synonyms: B230337F23Rik; C77483; Calnuc; MTEST82; Nucb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207335 representing NM_001163662
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTACCTCGGTGCCCCGCGGGGCCCCTTTTCTTCTCCTACCACCTCTACTGATGCTGTCTGCTGTGC
  TGGCAGTGCCCGTGGACCGCGCAGCACCTCCTCAGGAGGACAGCCAGGCCACTGAGACCCCGGACACGGG
  CCTGTACTACCACCGGTACCTCCAGGAGGTCATCAACGTGCTAGAGACAGATGGGCACTTCCGGGAGAAG
  CTGCAGGCTGCCAACGCTGAGGACATTAAGAGTGGAAAGCTGAGCCAAGAGCTGGACTTCGTCAGCCACA
  ACGTCCGAACCAAGCTGGATGAGCTCAAGCGACAGGAGGTGTCGAGGCTGCGGATGCTGCTCAAGGCCAA
  GATGGATGCAAAGCAGGAGCCCAACTTGCAGGTGGACCACATGAACCTCCTGAAGCAGTTTGAGCACCTG
  GACCCTCAGAACCAGCACACGTTTGAGGCTCGGGACCTAGAGCTGCTGATCCAGACGGCCACCCGAGACC
  TCGCCCAGTATGACGCTGCACACCATGAAGAGTTCAAACGCTACGAGATGCTCAAGGAACATGAGAGGAG
  ACGATACCTGGAGTCTCTGGGAGAGGAGCAGCGGAAGGAGGCCGAGAGGAAGCTCCAAGAGCAACAGCGC
  AGACACCGGGAACACCCCAAAGTCAATGTTCCTGGCAGCCAAGCCCAGTTGAAGGAGGTGTGGGAGGAGT
  TGGATGGATTGGACCCCAACAGGTTCAACCCCAAGACCTTCTTCATACTGCATGACATCAACAGTGATGG
  TGTCCTAGATGAGCAAGAACTGGAAGCTCTCTTTACCAAGGAGCTGGAGAAGGTGTATGACCCGAAGAAT
  GAGGAGGATGACATGAGAGAGATGGAGGAAGAACGGCTTCGCATGCGGGAGCACGTGATGAAGAATGTGG
  ACACCAACCAGGACCGGCTTGTGACCCTGGAGGAGTTCCTGGCATCCACCCAGAGGAAGGAGTTCGGGGA
  CACTGGGGAGGGGTGGAAGACAGTGGAAATGTCCCCAGCCTACACAGAGGAGGAGCTGAAGCGTTTTGAA
  GAGGAGCTGGCTGCCCGGGAAGCTGAGCTGAATGCCAGGGCCCAGCGCCTCAGCCAGGAGACAGAGGCCC
  TGGGGCGCTCCCAGGACCGCCTGGAGGCACAGAAGAGAGAGCTGCAGCAGGCTGTTCTGCAGATGGAGCA
  GAGGAAGCAGCAACTGCAAGAACAGAGCGCTCCGCCTTCCAAACCTGACGGGCAGCTGCAGTTCCGTGCA
  GACACAGATGACGCTCCTGTCCCAGCTCCAGCAGGTGACCAGAAAGATGTGCCTGCTTCTGAGAAGAAGG
  TCCCAGAGCAGCCCCCTGAGCTGCCACAGCTGGATTCCCAGCATTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207335 representing NM_001163662
  Red=Cloning site Green=Tags(s)
  
  MPTSVPRGAPFLLLPPLLMLSAVLAVPVDRAAPPQEDSQATETPDTGLYYHRYLQEVINVLETDGHFREK
  LQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPNLQVDHMNLLKQFEHL
  DPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQR
  RHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKN
  EEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWKTVEMSPAYTEEELKRFE
  EELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQLQEQSAPPSKPDGQLQFRA
  DTDDAPVPAPAGDQKDVPASEKKVPEQPPELPQLDSQHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163662
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163662.1, NP_001157134.1
• RefSeq Size: 2639 bp
• RefSeq ORF: 1380 bp
• Locus ID: 18220
• UniProt ID: Q02819
• Cytogenetics: 7 B3
• MW: 53.9 kDa
• Gene Summary: Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis (By similarity).[UniProtKB/Swiss-Prot Function]