Smpdl3b (NM_133888) Mouse Tagged ORF Clone

CAT#: MR207286

Smpdl3b (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase, acid-like 3B (Smpdl3b)

Product Images

Circular map for MR207286

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smpdl3b
• Synonyms: 1110054A24Rik; Asml3b; AU045240
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207286 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCTGCTCGGGTGGCTGATATTCCTGGCCCCCTGGGGAGTCGCAGGGGCTCAACTAGGGAGGTTCT
  GGCACATCTCCGACCTGCATCTGGACCCCAACTACACCGTATCCAAAGACCCCCTCCAGGTGTGCCCGTC
  GGCCGGCTCCCAGCCTGTGCTAAATGCTGGCCCCTGGGGGGACTACCTCTGCGATTCTCCTTGGGCCCTT
  ATCAACTCGTCCTTGTATGCCATGAAGGAGATTGAACCAAAGCCTGACTTCATTCTCTGGACAGGGGACG
  ACACACCGCACGTCCCCAATGAGAGTCTAGGAGAGGCAGCTGTGCTGGCAATTGTGGAACGCTTGACCAA
  CCTCATCAAGGAAGTCTTTCCAGACACTAAAGTCTATGCTGCTCTGGGAAATCATGACTTCCACCCTAAG
  AACCAGTTCCCAGCACAGAGCAACCGCATCTATAACCAGGTGGCAGAGCTGTGGAGACCCTGGCTTAGTA
  ACGAATCCTACGCTCTCTTCAAAAGAGGTGCCTTCTATTCTGAGAAGTTGCCGGGTCCCAGCAGGGCGGG
  GCGAGTTGTGGTCCTCAACACCAATCTGTACTACAGCAACAACGAGCAGACAGCTGGCATGGCTGACCCC
  GGCGAGCAGTTCCGGTGGCTGGGAGATGTCCTGAGCAATGCATCTCGGGATGGGGAGATGGTGTATGTTA
  TTGGCCACGTGCCCCCGGGGTTCTTTGAGAAGACACAGAACAAGGCCTGGTTCCGAGAGAGCTTCAATGA
  GGAGTATCTGAAGGTGATCCAGAAGCACCATCGGGTCATAGCGGGGCAGTTCTTTGGACACCACCATACC
  GACAGCTTCCGAATGTTCTATGACAACACAGGTGCCCCCATAAACGTCATGTTTCTCACACCCGGGGTCA
  CACCGTGGAAGACCACATTACCTGGAGTGGTCGATGGGGCCAACAATCCAGGGATACGCATTTTCGAGTA
  TGATCGAGCCACACTCAACTTGAAGGACTTGGTGACTTACTTCTTGAACCTGAGGCAGGCGAATGTACAA
  GAGACCCCACGGTGGGAGCAGGAGTACCGCCTGACGGAGGCCTACCAGGTGCCGGATGCCAGCGTCAGCT
  CCATGCACACGGCGCTGACCCGCATTGCCAGTGAGCCTCACATCCTGCAACGTTATTACGTCTATAACTC
  GGTCAGCTACAACCATTTGACCTGTGAGGACAGCTGCCGCATCGAGCACGTTTGTGCTATACAACACGTG
  GCTTTCAACACTTATGCTACCTGCTTGCATGGTCTTGGTGCCAAGCTGGTGCCTGGTTTCCTGCTCATAC
  TGACTCTGCTGCCAAGCCTGCACGTACTGGAGGTGTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207286 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTLLGWLIFLAPWGVAGAQLGRFWHISDLHLDPNYTVSKDPLQVCPSAGSQPVLNAGPWGDYLCDSPWAL
  INSSLYAMKEIEPKPDFILWTGDDTPHVPNESLGEAAVLAIVERLTNLIKEVFPDTKVYAALGNHDFHPK
  NQFPAQSNRIYNQVAELWRPWLSNESYALFKRGAFYSEKLPGPSRAGRVVVLNTNLYYSNNEQTAGMADP
  GEQFRWLGDVLSNASRDGEMVYVIGHVPPGFFEKTQNKAWFRESFNEEYLKVIQKHHRVIAGQFFGHHHT
  DSFRMFYDNTGAPINVMFLTPGVTPWKTTLPGVVDGANNPGIRIFEYDRATLNLKDLVTYFLNLRQANVQ
  ETPRWEQEYRLTEAYQVPDASVSSMHTALTRIASEPHILQRYYVYNSVSYNHLTCEDSCRIEHVCAIQHV
  AFNTYATCLHGLGAKLVPGFLLILTLLPSLHVLEVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133888
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133888.1
• RefSeq Size: 1925 bp
• RefSeq ORF: 1371 bp
• Locus ID: 100340
• UniProt ID: P58242
• Cytogenetics: 4 D2.3
• MW: 51.6 kDa
• Gene Summary: Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity (PubMed:26095358). Acts as a negative regulator of Toll-like receptor signaling (PubMed:26095358, PubMed:27687724). Has in vitro phosphodiesterase activity, but the physiological substrate is unknown (PubMed:26095358, PubMed:27687724). Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro) (PubMed:27687724).[UniProtKB/Swiss-Prot Function]