Angptl3 (NM_013913) Mouse Tagged ORF Clone

CAT#: MR207262

Angptl3 (Myc-DDK-tagged) - Mouse angiopoietin-like 3 (Angptl3)

Product Images

Circular map for MR207262

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Angptl3
• Synonyms: hy; hypl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207262 representing NM_013913
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACACAATTAAATTATTCCTTTTTGTTGTTCCTTTAGTAATTGCATCCAGAGTGGATCCAGACCTTT
  CATCATTTGATTCTGCACCTTCAGAGCCAAAATCAAGATTTGCTATGTTGGATGATGTCAAAATTTTAGC
  GAATGGCCTCCTGCAGCTGGGTCATGGACTTAAAGATTTTGTCCATAAGACTAAGGGACAAATTAACGAC
  ATATTTCAGAAGCTCAACATATTTGATCAGTCTTTTTATGACCTATCACTTCGAACCAATGAAATCAAAG
  AAGAGGAAAAGGAGCTAAGAAGAACTACATCTACACTACAAGTTAAAAACGAGGAGGTGAAGAACATGTC
  AGTAGAACTGAACTCAAAGCTTGAGAGTCTGCTGGAAGAGAAGACAGCCCTTCAACACAAGGTCAGGGCT
  TTGGAGGAGCAGCTAACCAACTTAATTCTAAGCCCAGCTGGGGCTCAGGAGCACCCAGAAGTAACATCAC
  TCAAAAGTTTTGTAGAACAGCAAGACAACAGCATAAGAGAACTCCTCCAGAGTGTGGAAGAACAGTATAA
  ACAATTAAGTCAACAGCACATGCAGATAAAAGAAATAGAAAAGCAGCTCAGAAAGACTGGTATTCAAGAA
  CCCTCAGAAAATTCTCTTTCTTCTAAATCAAGAGCACCAAGAACTACTCCCCCTCTTCAACTGAACGAAA
  CAGAAAATACAGAACAAGATGACCTTCCTGCCGACTGCTCTGCCGTTTATAACAGAGGCGAACATACAAG
  TGGCGTGTACACTATTAAACCAAGAAACTCCCAAGGGTTTAATGTCTACTGTGATACCCAATCAGGCAGT
  CCATGGACATTAATTCAACACCGGAAAGATGGCTCACAGGACTTCAACGAAACATGGGAAAACTACGAAA
  AGGGCTTTGGGAGGCTCGATGGAGAATTTTGGTTGGGCCTAGAGAAGATCTATGCTATAGTCCAACAGTC
  TAACTACATTTTACGACTCGAGCTACAAGACTGGAAAGACAGCAAGCACTACGTTGAATACTCCTTTCAC
  CTGGGCAGTCACGAAACCAACTACACGCTACATGTGGCTGAGATTGCTGGCAATATCCCCGGGGCCCTCC
  CAGAGCACACAGACCTGATGTTTTCTACATGGAATCACAGAGCAAAGGGACAGCTCTACTGTCCAGAAAG
  TTACTCAGGTGGCTGGTGGTGGAATGACATATGTGGAGAAAACAACCTAAATGGAAAATACAACAAACCC
  AGAACCAAATCCAGACCAGAGAGAAGAAGAGGGATCTACTGGAGACCTCAGAGCAGAAAGCTCTATGCTA
  TCAAATCATCCAAAATGATGCTCCAGCCCACCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207262 representing NM_013913
  Red=Cloning site Green=Tags(s)
  
  MHTIKLFLFVVPLVIASRVDPDLSSFDSAPSEPKSRFAMLDDVKILANGLLQLGHGLKDFVHKTKGQIND
  IFQKLNIFDQSFYDLSLRTNEIKEEEKELRRTTSTLQVKNEEVKNMSVELNSKLESLLEEKTALQHKVRA
  LEEQLTNLILSPAGAQEHPEVTSLKSFVEQQDNSIRELLQSVEEQYKQLSQQHMQIKEIEKQLRKTGIQE
  PSENSLSSKSRAPRTTPPLQLNETENTEQDDLPADCSAVYNRGEHTSGVYTIKPRNSQGFNVYCDTQSGS
  PWTLIQHRKDGSQDFNETWENYEKGFGRLDGEFWLGLEKIYAIVQQSNYILRLELQDWKDSKHYVEYSFH
  LGSHETNYTLHVAEIAGNIPGALPEHTDLMFSTWNHRAKGQLYCPESYSGGWWWNDICGENNLNGKYNKP
  RTKSRPERRRGIYWRPQSRKLYAIKSSKMMLQPTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013913
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013913.1, NM_013913.2, NM_013913.3, NM_013913.4, NP_038941.1
• RefSeq Size: 1605 bp
• RefSeq ORF: 1368 bp
• Locus ID: 30924
• UniProt ID: Q9R182
• Cytogenetics: 4 45.6 cM
• MW: 53 kDa
• Gene Summary: This gene encodes a member of the angiopoietin-like family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may inhibit triglyceride metabolism. Homozygous knockout mice for this gene exhibit reduced plasma lipid concentrations, including reduced plasma triglyceride concentrations, and enhanced activity of enzymes involved in triglyceride metabolism. [provided by RefSeq, Aug 2015]