Arih1 (NM_019927) Mouse Tagged ORF Clone

CAT#: MR207099

Arih1 (Myc-DDK-tagged) - Mouse ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1 (Drosophila) (Arih1)

Product Images

Circular map for MR207099

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arih1
• Synonyms: Ari; Ari1; AU021774; Hari; Hhari; Ubch7bp; Uip77
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207099 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAATGTATCCGGGAGGTCAACGAGGTCATCCAGAATCCAGCAACTATCACGAGAATACTCCTTA
  GCCACTTCAATTGGGATAAAGAGAAGCTAATGGAAAGGTACTTTGATGGAAACCTGGAGAAGCTCTTTGC
  TGAGTGTCATGTAATTAATCCAAGTAAAAAGTCTCGAACTCGCCAGATGAATACAAGGTCATCGGCACAG
  GATATGCCGTGTCAGATCTGCTACTTGAACTACCCTAACTCGTATTTTACTGGCCTTGAATGTGGACATA
  AGTTTTGTATGCAGTGCTGGAGTGAATATTTAACTACAAAAATAATGGAGGAAGGCATGGGGCAGACTAT
  TTCGTGTCCTGCGCATGGTTGTGATATCTTAGTGGATGACAACACAGTTATGCGCCTGATCACAGATTCC
  AAAGTTAAGTTAAAATATCAACATTTAATAACAAATAGCTTTGTAGAGTGCAACCGACTGTTAAAGTGGT
  GTCCTGCCCCAGATTGCCACCATGTTGTTAAAGTCCAGTATCCTGATGCAAAACCAGTTCGCTGCAAATG
  TGGCCGCCAGTTTTGCTTTAACTGTGGTGAAAATTGGCATGATCCAGTTAAATGTAAGTGGTTGAAGAAG
  TGGATTAAAAAGTGTGATGATGACAGTGAAACTTCTAATTGGATTGCAGCTAACACAAAGGAATGTCCCA
  AATGCCATGTCACAATTGAGAAGGATGGCGGTTGTAATCACATGGTCTGTCGTAACCAGAACTGTAAAGC
  AGAATTTTGCTGGGTGTGTCTTGGCCCTTGGGAACCGCATGGATCTGCCTGGTACAACTGTAACCGCTAT
  AATGAGGATGATGCAAAGGCAGCAAGAGATGCCCAAGAGCGATCTAGGGCGGCCCTGCAGAGATACCTGT
  TCTACTGTAATCGCTATATGAACCACATGCAGAGTCTACGCTTCGAGCATAAGCTGTATGCTCAGGTGAA
  ACAGAAGATGGAGGAGATGCAGCAGCACAACATGTCCTGGATCGAGGTGCAGTTCCTGAAGAAAGCAGTT
  GATGTCCTCTGCCAGTGTCGTGCCACACTCATGTACACTTATGTCTTCGCTTTCTACCTCAAAAAGAATA
  ACCAGTCCATTATCTTTGAGAATAATCAAGCAGATCTAGAAAATGCCACAGAGGTGCTTTCGGGCTACCT
  TGAACGAGATATTTCCCAAGATTCTCTGCAAGATATAAAGCAGAAAGTCCAAGATAAGTACAGATACTGT
  GAGAGTCGACGAAGGGTTTTGTTACAGCATGTGCATGAAGGCTATGAAAAAGATCTGTGGGAGTACATTG
  AGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207099 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVECIREVNEVIQNPATITRILLSHFNWDKEKLMERYFDGNLEKLFAECHVINPSKKSRTRQMNTRSSAQ
  DMPCQICYLNYPNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMGQTISCPAHGCDILVDDNTVMRLITDS
  KVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCKCGRQFCFNCGENWHDPVKCKWLKK
  WIKKCDDDSETSNWIAANTKECPKCHVTIEKDGGCNHMVCRNQNCKAEFCWVCLGPWEPHGSAWYNCNRY
  NEDDAKAARDAQERSRAALQRYLFYCNRYMNHMQSLRFEHKLYAQVKQKMEEMQQHNMSWIEVQFLKKAV
  DVLCQCRATLMYTYVFAFYLKKNNQSIIFENNQADLENATEVLSGYLERDISQDSLQDIKQKVQDKYRYC
  ESRRRVLLQHVHEGYEKDLWEYIED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019927
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019927.1, NP_064311.1
• RefSeq Size: 6435 bp
• RefSeq ORF: 1668 bp
• Locus ID: 23806
• UniProt ID: Q9Z1K5
• Cytogenetics: 9 B
• MW: 52.7 kDa
• Gene Summary: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-RING ubiquitin ligase (CRL) complexes and initiating ubiquitination of CRL substrates: associates with CRL complexes and specifically mediates addition of the first ubiquitin on CRLs targets. The initial ubiquitin is then elongated by CDC34/UBE2R1 and UBE2R2. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated cullin-RING ubiquitin ligase complexes. Plays a role in protein translation in response to DNA damage by mediating ubiquitination of EIF4E2, the consequences of EIF4E2 ubiquitination are however unclear. According to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest. According to another report EIF4E2 ubiquitination leads to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2.[UniProtKB/Swiss-Prot Function]