Faf2 (NM_178397) Mouse Tagged ORF Clone

CAT#: MR207077

Faf2 (Myc-DDK-tagged) - Mouse Fas associated factor family member 2 (Faf2)

Product Images

Circular map for MR207077

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Faf2
• Synonyms: 2210404D11Rik; AI462440; mKIAA0887; Ubxd8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207077 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCTGAGGAGCAGGATCTAACTCAGGAGCAGACCGAGAAGCTGCTGCAGTTTCAGGATCTGA
  CTGGCATAGAGTCCATGGAGCAGTGTCGCCTTGCTTTGGAGCAGCATAACTGGAACATGGAGGCTGCTGT
  CCAGGACAGACTGAATGAGCAGGAAGGTGTACCAAGTGTTTTCAATCCACCGCCAGCTCGGCCCCTACAG
  GTTACAGCTGACCACAGGATCTACAGCTATGTTGTCTCAAGACCACAACCAAGGGGCCTGCTTGGATGGG
  GTTATTACTTGATAATGCTTCCATTCCGGTTTACCTATTACACGATACTTGATATATTTAGGTTTGCTCT
  TCGTTTTATACGGCCTGACCCTCGCAGCCGGGTCACTGACCCTGTTGGGGACATTGTTTCATTTATGCAC
  TCTTTTGAAGAGAAATATGGGAGGGCACACCCTGTCTTCTACCAGGGAACGTACAGCCAGGCACTTAACG
  ATGCCAAGCGGGAACTTCGATTTCTTTTGGTTTATCTTCATGGAGATGACCACCAGGACTCTGATGAGTT
  CTGCCGCAATGCTCTCTGCGCACCTGAGGTCATCTCACTGATAAACAGCAGGATGCTTTTCTGGGCTTGT
  TCTACAAACAAGCCTGAGGGGTACAGGGTGTCCCAGGCCTTACGGGAGAACACCTATCCATTCCTAGCTA
  TGATTATGTTGAAGGATCGGCGAATGACTGTGGTTGGAAGGCTAGAAGGCCTCATTCAACCTGATGACCT
  CATCAACCAGCTGACATTCATCATGGATGCAAACCAGACTTACCTGGTGTCAGAACGTCTAGAAAGGGAA
  GAAAGGAACCAAACCCAGGTGCTGAGACAACAGCAGGATGAAGCCTACCTGGCTTCTCTCAGGGCAGATC
  AGGAGAAAGAGAGGAAGAAGAGGGAGGAGAAAGAGCGGAAACGCCGGAAGGAGGAGGAGGTGCAGCAACA
  GAAGCTGGCGGAGGAGCGAAGACGACAGAACTTGCAGGAGGAAAAGGAGAGGAAGCTGGAATGCCTGCCC
  CCTGAGCCCTCCCCTGATGATCCTGAAAGTGTCAAGATCATTTTCAAGTTACCCAATGATTCCAGAGTAG
  AGAGACGATTCCACTTCTCACAGTCTCTAACAGTCATCCATGACTTCTTATTCTCCTTGAAGGAAAGCCC
  TGAAAAGTTTCAGATTGAAGCCAATTTTCCAAGACGGGTACTTCCCTGTGTTCCTTCAGAGGAATGGCCC
  AACCCCCCAACACTGCAGGAGGCGGGACTCAGCCACACAGAAGTTCTCTTTGTCCAGGACCTGACAGACG
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207077 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPEEQDLTQEQTEKLLQFQDLTGIESMEQCRLALEQHNWNMEAAVQDRLNEQEGVPSVFNPPPARPLQ
  VTADHRIYSYVVSRPQPRGLLGWGYYLIMLPFRFTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMH
  SFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNALCAPEVISLINSRMLFWAC
  STNKPEGYRVSQALRENTYPFLAMIMLKDRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLERE
  ERNQTQVLRQQQDEAYLASLRADQEKERKKREEKERKRRKEEEVQQQKLAEERRRQNLQEEKERKLECLP
  PEPSPDDPESVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCVPSEEWP
  NPPTLQEAGLSHTEVLFVQDLTDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178397
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178397.2, NP_848484.1
• RefSeq Size: 4322 bp
• RefSeq ORF: 1338 bp
• Locus ID: 76577
• UniProt ID: Q3TDN2
• Cytogenetics: 13 B1
• MW: 52.4 kDa
• Gene Summary: Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis.[UniProtKB/Swiss-Prot Function]