Ddost (NM_007838) Mouse Tagged ORF Clone

CAT#: MR207041

Ddost (Myc-DDK-tagged) - Mouse dolichyl-di-phosphooligosaccharide-protein glycotransferase (Ddost)

Product Images

Circular map for MR207041

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ddost
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207041 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGGATCCCCGCCTCGCCGTCCGCGCCTGGCCCCTCTGCGGGCTGCTGCTGGCCGTGCTCGGCT
  GCGTCTGCGCCAGCGGCCCCCGCACCCTCGTGCTGCTGGACAACCTGAACGTGCGGGACACGCACTCGCT
  GTTCTTCCGCAGCCTGAAGGACCGGGGCTTTGAGCTCACCTTCAAGACCGCAGATGACCCCAGCTTGTCC
  CTCATTAAGTACGGGGAGTTCCTCTATGACAACCTTATCATCTTTTCCCCGTCGGTGGAAGATTTTGGAG
  GCAACATCAACGTGGAGACCATCAGTGCCTTCATTGATGGTGGCGGCAGCGTTTTGGTGGCTGCCAGCTC
  TGATATTGGTGACCCTCTTCGGGAGCTGGGCAGTGAGTGTGGGATTGAATTTGATGAAGAGAAAACAGCT
  GTCATCGACCACCACAACTATGATGTTTCTGACCTTGGCCAGCACACACTCATTGTGGCTGACACTGAGA
  ACCTGCTGAAGGCCCCGACCATTGTTGGCAAGTCATCTCTGAACCCCATTCTCTTCCGAGGAGTTGGAAT
  GGTGGCAGACCCGGACAATCCCTTGGTTTTGGACATCCTAACAGGCTCTTCAACCTCTTACTCCTTCTTC
  CCAGATAAACCAATCACCCAGTACCCCCATGCGGTGGGGAGGAACACTCTGCTGATTGCCGGGCTCCAGG
  CCAGGAACAACGCCCGGGTCATCTTCAGTGGCTCTCTGGATTTCTTCAGCGATGCCTTCTTCAACTCGGC
  AGTGCAGAAGGCCACACCCGGTGCGCAGAGGTATTCTCAGACAGGCAACTATGAACTAGCTGTGGCCCTC
  TCACGCTGGGTGTTCAAGGAGGAGGGTGTCCTTCGAGTAGGGCCTGTGTCCCATCACCGGGTGGGCGAGA
  TGGCTCCACCCAATGCCTACACTGTCACCGACTTGGTGGAGTATAGCATCATCATAGAACAGCTCTCCAA
  TGGCAAGTGGGTCCCCTTTGATGGTGATGACATTCAGCTGGAGTTCGTGCGCATCGACCCCTTCGTGAGG
  ACCTTCCTGAAGAGGAAAGGTGGCAAGTACAGTGTCCAGTTCAAGCTGCCTGACGTGTATGGTGTATTCC
  AGTTTAAAGTGGATTACAACCGGCTAGGCTACACCCACCTGTACTCTTCCACCCAGGTGTCAGTGAGGCC
  ACTCCAGCACACGCAGTATGAGCGCTTCATCCCCTCGGCCTATCCCTACTATGCCAGTGCCTTCTCCATG
  ATGGCCGGGCTCTTCATCTTCAGCATCGTCTTCTTGCACATGAAGGAGAAGGAGAAGTCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207041 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKMDPRLAVRAWPLCGLLLAVLGCVCASGPRTLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLS
  LIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTA
  VIDHHNYDVSDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFF
  PDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQTGNYELAVAL
  SRWVFKEEGVLRVGPVSHHRVGEMAPPNAYTVTDLVEYSIIIEQLSNGKWVPFDGDDIQLEFVRIDPFVR
  TFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSM
  MAGLFIFSIVFLHMKEKEKSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007838
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007838.3
• RefSeq Size: 2134 bp
• RefSeq ORF: 1326 bp
• Locus ID: 13200
• UniProt ID: O54734
• Cytogenetics: 4 D3
• MW: 49 kDa
• Gene Summary: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes (By similarity).[UniProtKB/Swiss-Prot Function]