Ccnb1 (NM_172301) Mouse Tagged ORF Clone

CAT#: MR206857

Ccnb1 (Myc-DDK-tagged) - Mouse cyclin B1 (Ccnb1)

Product Images

Circular map for MR206857

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccnb1
• Synonyms: Ccnb1-rs1; Ccnb1-rs13; Cycb-4; Cycb-5; CycB1; Cycb1-rs1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206857 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCAGGGTCACTAGGAACACGAAAATTAACGCAGAAAATAAGGCCAAGGTCAGTATGGCAGGCG
  CCAAGCGTGTGCCTGTGACAGTTACTGCTGCTTCCAAGCCCGGGCTGAGACCGAGAACTGCTCTTGGAGA
  CATTGGTAATAAAGTCAGCGAAGAGCTACAGGCAAGAGTGCCTCTGAAAAGGGAAGCAAAAACGCTAGGT
  ACTGGAAAAGGTACTGTTAAAGCCCTACCAAAACCTGTAGAGAAGGTGCCTGTGTGTGAACCAGAGGTGG
  AACTTGCTGAGCCTGAGCCTGAACCTGAACTTGAACATGTTAGAGAAGAGAAGCTTTCTCCTGAACCTAT
  TTTGGTTGATAATCCCTCTCCAAGCCCGATGGAAACATCTGGATGTGCGCCTGCAGAAGAGTATCTGTGT
  CAGGCTTTCTCTGATGTAATCCTTGCAGTGAGTGACGTAGACGCAGATGATGGGGCTGACCCAAACCTCT
  GTAGTGAATATGTGAAAGATATCTATGCTTATCTCCGACAACTGGAGGAAGAGCAGTCAGTTAGACCAAA
  ATACCTACAGGGTCGTGAAGTGACTGGAAACATGAGAGCTATCCTCATTGACTGGCTAATACAGGTTCAG
  ATGAAATTTAGGCTGCTTCAGGAGACCATGTACATGACTGTGTCCATTATTGATCGGTTCATGCAGAACA
  GTTGTGTGCCCAAGAAGATGCTACAGCTGGTCGGTGTAACGGCCATGTTTATTGCAAGCAAATATGAGGA
  GATGTACCCTCCAGAAATAGGTGACTTCGCCTTTGTGACTAACAACACGTACACTAAGCACCAGATCAGA
  CAGATGGAGATGAAGATTCTCAGAGTTCTGAACTTCAGCCTGGGTCGCCCTCTGCCTCTGCACTTCCTCC
  GTAGAGCATCTAAAGTCGGAGAGGTTGACGTCGAGCAGCACACTTTGGCCAAATACCTCATGGAGCTCTC
  CATGCTGGACTACGACATGGTGCATTTTGCTCCTTCTCAAATTGCAGCTGGGGCTTTCTGCTTAGCGCTG
  AAAATTCTTGACAACGGTGAATGGACACCAACTCTGCAGCACTACCTATCCTACAGTGAAGACTCCCTGC
  TTCCTGTTATGCAGCACCTGGCTAAGAATGTAGTCATGGTGAACTGTGGCCTCACAAAGCACATGACTGT
  CAAGAACAAGTATGCAGCATCTAAGCATGCTAAGATTAGCACGCTGGCACAGCTGAACTGTACACTAGTT
  CAGAATTTGTCTAAGGCCGTGACAAAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206857 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALRVTRNTKINAENKAKVSMAGAKRVPVTVTAASKPGLRPRTALGDIGNKVSEELQARVPLKREAKTLG
  TGKGTVKALPKPVEKVPVCEPEVELAEPEPEPELEHVREEKLSPEPILVDNPSPSPMETSGCAPAEEYLC
  QAFSDVILAVSDVDADDGADPNLCSEYVKDIYAYLRQLEEEQSVRPKYLQGREVTGNMRAILIDWLIQVQ
  MKFRLLQETMYMTVSIIDRFMQNSCVPKKMLQLVGVTAMFIASKYEEMYPPEIGDFAFVTNNTYTKHQIR
  QMEMKILRVLNFSLGRPLPLHFLRRASKVGEVDVEQHTLAKYLMELSMLDYDMVHFAPSQIAAGAFCLAL
  KILDNGEWTPTLQHYLSYSEDSLLPVMQHLAKNVVMVNCGLTKHMTVKNKYAASKHAKISTLAQLNCTLV
  QNLSKAVTKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172301
• ORF Size: 1293 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172301.2
• RefSeq Size: 2316 bp
• RefSeq ORF: 1293 bp
• Locus ID: 268697
• UniProt ID: P24860
• Cytogenetics: 13 53.23 cM
• MW: 48.1 kDa
• Gene Summary: Essential for the control of the cell cycle at the G2/M (mitosis) transition.[UniProtKB/Swiss-Prot Function]