E2f1 (NM_007891) Mouse Tagged ORF Clone
CAT#: MR206856
- TrueORF®
E2f1 (Myc-DDK-tagged) - Mouse E2F transcription factor 1 (E2f1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_007891" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | E2f1 |
Synonyms | E2F-1; mKIAA4009; Tg(Wnt1-cre)2Sor |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206856 representing NM_007891
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTAGCCCCCGCGGGCGGCCAGCACGCGCCAGCGCTGGAGGCCCTGCTCGGGGCGGGCGCGTTGC GGCTGCTCGACTCCTCGCAGATCGTCATCATCTCCACCGCGCCCGATGTCGGCGCCCCGCAGCTCCCCGC CGCGCCGCCCACTGGCCCTCGCGATTCTGACGTGCTGCTCTTCGCCACGCCGCAGGCGCCCCGACCCGCG CCTAGTGCACCGCGCCCGGCTCTCGGCCGCCCGCCGGTGAAACGGAGGCTGGATCTGGAGACTGACCATC AGTACCTCGCTGGTAGCAGTGGGCCATTCCGGGGCAGAGGCCGCCACCCAGGGAAAGGTGTGAAATCTCC GGGGGAGAAGTCACGCTATGAAACCTCACTAAATCTGACCACCAAACGCTTCTTGGAGCTGCTGAGCCGC TCAGCTGACGGTGTCGTTGACCTGAACTGGGCAGCTGAGGTGCTGAAGGTGCAGAAACGGCGCATCTATG ACATCACCAATGTCCTGGAGGGCATCCAGCTCATTGCCAAGAAGTCCAAGAATCATATCCAGTGGCTAGG CAGCCACACCATGGTGGGGATTGGTAAGCGGCTTGAAGGCCTGACCCAGGACCTGCAGCAACTGCAGGAG AGTGAGCAGCAGCTGGATCACCTGATGCACATCTGTACCACACAGCTGCAACTGCTTTCGGAGGACTCCG ACACCCAGCGCCTGGCCTATGTGACCTGCCAGGACCTTCGCAGCATTGCAGACCCTGCAGAACAGATGGT CATAGTGATCAAGGCCCCTCCTGAGACCCAACTACAAGCTGTGGATTCTTCAGAGACATTTCAGATCTCC CTTAAGAGCAAACAAGGCCCCATTGATGTTTTCCTGTGCCCGGAGGAGAGTGCAGACGGGATTAGCCCTG GGAAGACCTCATGCCAGGAGACATCCTCTGGGGAGGACCGGACTGCAGACTCTGGCCCAGCAGGGCCTCC ACCATCACCTCCCTCCACATCCCCAGCCTTGGATCCCAGTCAATCCCTGTTGGGCCTGGAGCAAGAAGCA GTATTGCCACGGATGGGCCACCTGAGGGTCCCTATGGAAGAGGACCAACTGTCACCACTGGTGGCTGCTG ACTCACTCCTGGAGCATGTTAAAGAAGACTTCTCTGGGCTCCTCCCTGGGGAGTTCATCAGCCTCTCCCC ACCCCACGAGGCCCTTGACTATCACTTTGGTCTCGAGGAGGGTGAGGGCATTAGAGATCTCTTTGACTGT GACTTTGGGGACCTGACCCCTCTGGATTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206856 representing NM_007891
Red=Cloning site Green=Tags(s) MAVAPAGGQHAPALEALLGAGALRLLDSSQIVIISTAPDVGAPQLPAAPPTGPRDSDVLLFATPQAPRPA PSAPRPALGRPPVKRRLDLETDHQYLAGSSGPFRGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSR SADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTMVGIGKRLEGLTQDLQQLQE SEQQLDHLMHICTTQLQLLSEDSDTQRLAYVTCQDLRSIADPAEQMVIVIKAPPETQLQAVDSSETFQIS LKSKQGPIDVFLCPEESADGISPGKTSCQETSSGEDRTADSGPAGPPPSPPSTSPALDPSQSLLGLEQEA VLPRMGHLRVPMEEDQLSPLVAADSLLEHVKEDFSGLLPGEFISLSPPHEALDYHFGLEEGEGIRDLFDC DFGDLTPLDF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_007891 |
ORF Size | 1290 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007891.5 |
RefSeq Size | 2469 bp |
RefSeq ORF | 1293 bp |
Locus ID | 13555 |
UniProt ID | Q61501 |
Cytogenetics | 2 76.79 cM |
MW | 46.8 kDa |
Gene Summary | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:11672531, PubMed:20176812). Positively regulates transcription of RRP1B (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208010 | E2f1 (untagged) - Mouse E2F transcription factor 1 (E2f1), (10ug) |
USD 457.00 |
|
MG206856 | E2f1 (tGFP-tagged) - Mouse E2F transcription factor 1 (E2f1) |
USD 657.00 |
|
MR206856L3 | Lenti ORF clone of E2f1 (Myc-DDK-tagged) - Mouse E2F transcription factor 1 (E2f1) |
USD 757.00 |
|
MR206856L4 | Lenti ORF clone of E2f1 (mGFP-tagged) - Mouse E2F transcription factor 1 (E2f1) |
USD 757.00 |
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