Cpa4 (NM_027926) Mouse Tagged ORF Clone

CAT#: MR206686

Cpa4 (Myc-DDK-tagged) - Mouse carboxypeptidase A4 (Cpa4)

Product Images

Circular map for MR206686

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cpa4
• Synonyms: 1110019K20Rik; AV009555
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206686 representing NM_027926
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGGCTACTGTTCTTTGGGGCCCTGATTGGGGCAGGCATCTGCGGCCGAGATAAATTCTTTGGGG
  ACCAAGTTTTTAGGATTAATGTCAGAAATGGAGACGAGATCAGAAAACTGACTGAGCTAGTGAATTCTGA
  CCACTTAAAGCTCAGTGTCTGGAAATCTCCCTCCACTTTTGATCGGCCCGTGGATATTCTCGTCCCTTCT
  GTCAGCTTGCTCCCAGTCAAGTCCTTCCTGAAGTCCCAGGGTTTAGACTACTCCGTGACAATTGAGGACC
  TCCAGGCCCTTTTAGATAACGAAGATGAAGAAATGCAGCACAATGAAGGGATAGAGCGGAGTGGTGACTT
  CAACTACGGAGCTTACCATCCCCTGGAGGCTATTTACCACGAGATGGACAGTATTGCCACAGACTTTCCT
  GAGCTGGTGAGCAGAGTGAAGATTGGAGAGACATTTGAAAAGCGGCCCATGTATGTTCTAAAGTTCAGCA
  CGGGAGGAGGCAAGAAGCGGCCAGCCATTTGGTTGAATGCAGGCATCCATGCCCGTGAGTGGATCTCACA
  GGCCACAGCCATCTGGACAGCGAGGAAGATTGTAACTGATTATAAAAAGGACCCGGCTATCACCTCCATC
  TTGAAGAAAGTGGATATTTTCTTGTTGCCCGTGGCCAATCCTGATGGATATGTGTACACGCAAAGCCAGA
  ACCGATTATGGAGAAAGACGCGGTCCCGGAACCCAGGAAGCCGCTGTGTTGGAGCCGATCCAAACAGAAA
  CTGGAATGCTAGTTTTGCAGGAGAGGGGACCAGTGATAACCCTTGCTCTGAAGTGTACCATGGCTCCCAC
  CCCAATTCTGAAGTGGAGGTGAAATCGGTGGTGGATTTCATTCAAAAGCATGGGAACTTCAAATGCTTCA
  TTGACCTGCATAGCTACTCACAGCTGCTGATGTATCCCTATGGGTACACAGTCAAGAAGGCCCCGGATGC
  TGAGGAGCTGGACGATGTGGCGAGGAATGCAGCCCAAGCCCTGGCTTCTCTCTCGGGCACTAAGTACCGA
  GTGGGCCCAACTTGCACCACCGTCTATCCAGCTAGTGGGAGCAGCGTTGACTGGGCATACGACAATGGCA
  TCAAGTATGCTTTCACATTTGAGCTGAGAGACACTGGGTACTACGGCTTCCTCTTGCCAGCCAGCCAGAT
  CATCCCCACCGCGGAGGAGACCTGGCTAGGGCTGAAGACCATCATGGAACATGTTCGGGACCACCTCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206686 representing NM_027926
  Red=Cloning site Green=Tags(s)
  
  MKWLLFFGALIGAGICGRDKFFGDQVFRINVRNGDEIRKLTELVNSDHLKLSVWKSPSTFDRPVDILVPS
  VSLLPVKSFLKSQGLDYSVTIEDLQALLDNEDEEMQHNEGIERSGDFNYGAYHPLEAIYHEMDSIATDFP
  ELVSRVKIGETFEKRPMYVLKFSTGGGKKRPAIWLNAGIHAREWISQATAIWTARKIVTDYKKDPAITSI
  LKKVDIFLLPVANPDGYVYTQSQNRLWRKTRSRNPGSRCVGADPNRNWNASFAGEGTSDNPCSEVYHGSH
  PNSEVEVKSVVDFIQKHGNFKCFIDLHSYSQLLMYPYGYTVKKAPDAEELDDVARNAAQALASLSGTKYR
  VGPTCTTVYPASGSSVDWAYDNGIKYAFTFELRDTGYYGFLLPASQIIPTAEETWLGLKTIMEHVRDHLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027926
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027926.3
• RefSeq Size: 2061 bp
• RefSeq ORF: 1263 bp
• Locus ID: 71791
• UniProt ID: Q6P8K8
• Cytogenetics: 6 A3.3
• MW: 47.8 kDa
• Gene Summary: This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases that could be involved in the histone hyperacetylation pathway. The encoded preproprotein undergoes proteolytic processing that removes the N-terminal activation peptide to generate a functional enzyme. This gene is located in a cluster of carboxypeptidase genes on chromosome 6. [provided by RefSeq, Jul 2016]