Irf3 (NM_016849) Mouse Tagged ORF Clone

CAT#: MR206641

  • TrueORF®

Irf3 (Myc-DDK-tagged) - Mouse interferon regulatory factor 3 (Irf3)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_016849" in other vectors (4)


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Reconstitution Protocol

USD 686.00

In Stock*

Size
    • 10 ug

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Frequently bought together (3)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Irf3
Synonyms C920001K05Rik; IRF-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206641 representing NM_016849
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACCCCGAAACCGCGGATTTTGCCCTGGCTGGTGTCACAGCTGGACCTGGGGCAGCTGGAAGGCG
TGGCCTGGCTGGACGAGAGCCGAACGAGGTTCAGGATCCCGTGGAAGCATGGCCTACGGCAGGACGCACA
GATGGCTGACTTTGGCATCTTCCAGGCCTGGGCAGAAGCCAGTGGTGCCTACACCCCGGGGAAGGATAAG
CCGGACGTGTCAACCTGGAAGAGGAATTTCCGGTCAGCCCTGAACCGGAAAGAAGTGTTGCGGTTAGCTG
CTGACAATAGCAAGGACCCTTATGACCCTCATAAAGTGTATGAGTTTGTGACTCCAGGGGCGCGGGACTT
CGTACATCTGGGTGCCTCTCCTGACACCAATGGCAAAAGCAGCCTGCCTCACTCCCAGGAAAACCTACCG
AAGTTATTTGATGGCCTGATCTTGGGGCCCCTCAAAGATGAGGGGTCCTCAGATCTGGCTATTGTTTCTG
ATCCTTCTCAACAACTGCCAAGCCCCAATGTGAACAACTTCCTAAACCCTGCACCCCAAGAAAATCCACT
GAAGCAGCTGCTAGCTGAGGAACAATGGGAGTTCGAGGTGACCGCCTTCTACCGAGGCCGCCAGGTCTTC
CAGCAGACACTCTTTTGCCCGGGGGGCCTGCGGCTGGTGGGCAGCACAGCTGACATGACACTGCCCTGGC
AGCCAGTCACCCTGCCCGATCCTGAGGGGTTTCTGACGGACAAGCTTGTGAAGGAGTACGTGGGGCAGGT
GCTCAAAGGGCTGGGCAATGGGCTGGCACTGTGGCAGGCTGGGCAGTGCCTCTGGGCCCAGCGCCTAGGC
CACTCCCACGCCTTCTGGGCTCTGGGGGAGGAGCTGCTTCCAGACAGTGGGCGAGGGCCTGATGGAGAGG
TCCACAAGGACAAGGACGGAGCCGTGTTCGACCTCAGGCCCTTCGTGGCAGATCTGATTGCCTTCATGGA
AGGAAGTGGACACTCCCCACGCTACACTCTGTGGTTCTGCATGGGGGAAATGTGGCCCCAGGACCAGCCA
TGGGTCAAGAGGCTTGTGATGGTCAAGGTTGTTCCTACATGTCTTAAGGAGCTGTTAGAGATGGCCCGGG
AAGGGGGAGCCTCTTCACTGAAAACCGTGGACTTGCACATCTCCAACAGCCAGCCTATCTCCCTTACCTC
TGACCAGTACAAGGCCTACCTCCAGGACTTGGTGGAGGACATGGACTTCCAGGCCACTGGAAATATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206641 representing NM_016849
Red=Cloning site Green=Tags(s)

METPKPRILPWLVSQLDLGQLEGVAWLDESRTRFRIPWKHGLRQDAQMADFGIFQAWAEASGAYTPGKDK
PDVSTWKRNFRSALNRKEVLRLAADNSKDPYDPHKVYEFVTPGARDFVHLGASPDTNGKSSLPHSQENLP
KLFDGLILGPLKDEGSSDLAIVSDPSQQLPSPNVNNFLNPAPQENPLKQLLAEEQWEFEVTAFYRGRQVF
QQTLFCPGGLRLVGSTADMTLPWQPVTLPDPEGFLTDKLVKEYVGQVLKGLGNGLALWQAGQCLWAQRLG
HSHAFWALGEELLPDSGRGPDGEVHKDKDGAVFDLRPFVADLIAFMEGSGHSPRYTLWFCMGEMWPQDQP
WVKRLVMVKVVPTCLKELLEMAREGGASSLKTVDLHISNSQPISLTSDQYKAYLQDLVEDMDFQATGNI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016849
ORF Size 1257 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016849.4
RefSeq Size 2041 bp
RefSeq ORF 1260 bp
Locus ID 54131
UniProt ID P70671
Cytogenetics 7 B3
MW 47.3 kDa
Gene Summary Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed:15800576). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed:15800576). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed:16846591, PubMed:16979567, PubMed:20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed:16846591, PubMed:16979567, PubMed:20049431). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed:16846591, PubMed:16979567, PubMed:20049431). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed:16846591, PubMed:16979567, PubMed:20049431).[UniProtKB/Swiss-Prot Function]

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