Alg2 (NM_019998) Mouse Tagged ORF Clone

CAT#: MR206559

Alg2 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 2 homolog (yeast, alpha-1,3-mannosyltransferase) (Alg2)

Product Images

Circular map for MR206559

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alg2
• Synonyms: 1110018A23Rik; 1300013N08Rik; ALPG2; CDGIi; MNCb-5081
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206559 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAAAACCTGTACCGAGCCCGTTCCCGGGTTTACAGCCCATCTGTGCTGTTTCTGCACCCAGACA
  TGGGTATAGGCGGAGCCGAGCGCCTAGTGCTGGACGCGGCGCTGGCGCTGCAGGAGTACGGCTGTGATGT
  GAAGATATGGACCGCGCACTACGACCCGAACCACTGCTTCATCGAGACCCGCGAGCTCTCGGTGCAATGC
  GCAGGGGACTGGCTGCCTCGCAGCCTGGGCTGGGGCGGCCGCGGCGCCGCCATCTGCTCCTACGTGCGCA
  TGGTCTTTCTGGCGCTCTACGTGCTGTTTCTCTCCGGCGAGGAGTTCGACGTGGTGGTGTGCGACCAGGT
  GTCTGCCTGTATCCCGGTGTTCAAACTGGCCAGACGGCGTAAGAGGGTCCTATTTTACTGTCACTTCCCA
  GATCTGCTGCTTACTCAGAGAAATTCAGCTCTGAAGAAGTTCTACAGGGCCCCCATCGACTGGATCGAGG
  AATACACCACAGGCATGGCAGACCGCATCTTGGTCAACAGCCAGTACACTGCTTCCGTCTTTAAAGAAAC
  CTTCAAGACCCTGTCTCACAGAAATCCTGATGTGCTCTACCCATCTCTGAATATCGGCAGCTTTGACTTG
  GCTATTCCTGAAAAGATAGATGACCTCGTCCCCAAGGGGAAGCAATTCCTGTTCCTCTCTATCAACCGAT
  ACGAAAGGAAGAAAAATCTGCCCTTGGCACTGAGATCCTTGGTGCAGCTTCGGAATCGGTTACCATCTCA
  AGAGTGGGATAAGGTTCATCTCTTCATGGCCGGTGGTTATGACGATAGGATCCCGGAGAACGTGGAGCAC
  TATAAGGAGTTGAAGAAAATGGTCCAAGAGTCAGACCTTGAGCGTCATGTGACCTTCCTGCGGTCCTTCT
  CGGACAGACAGAAGATCTCACTCCTCCACGGCTGCTTGTGTGTGCTCTACACTCCGAGCAACGAGCACTT
  TGGCATCGTCCCTCTGGAGGCCATGTACATGCAGTGCCCAGTCATCGCTGTTAATAACGGTGGGCCCCTG
  GAGTCCATTGTCCACAAGGTCACGGGGTTCCTGTGTGAGCCAGACCCAGTGCACTTCTCAGAAGCCATGG
  AGAAGTTCATCCACAAACCATCCTTAAAAGCCACGATGGGCCTCGCTGGAAAAGCCAGGGTGGCGGAGAA
  GTTTTCAGCTGATGCATTTGCAGACCAGCTGTACCAGTATGTCACGAAGCTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206559 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAENLYRARSRVYSPSVLFLHPDMGIGGAERLVLDAALALQEYGCDVKIWTAHYDPNHCFIETRELSVQC
  AGDWLPRSLGWGGRGAAICSYVRMVFLALYVLFLSGEEFDVVVCDQVSACIPVFKLARRRKRVLFYCHFP
  DLLLTQRNSALKKFYRAPIDWIEEYTTGMADRILVNSQYTASVFKETFKTLSHRNPDVLYPSLNIGSFDL
  AIPEKIDDLVPKGKQFLFLSINRYERKKNLPLALRSLVQLRNRLPSQEWDKVHLFMAGGYDDRIPENVEH
  YKELKKMVQESDLERHVTFLRSFSDRQKISLLHGCLCVLYTPSNEHFGIVPLEAMYMQCPVIAVNNGGPL
  ESIVHKVTGFLCEPDPVHFSEAMEKFIHKPSLKATMGLAGKARVAEKFSADAFADQLYQYVTKLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019998
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019998.3
• RefSeq Size: 3062 bp
• RefSeq ORF: 1248 bp
• Locus ID: 56737
• UniProt ID: Q9DBE8
• Cytogenetics: 4 B1
• MW: 47.4 kDa
• Gene Summary: Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.[UniProtKB/Swiss-Prot Function]