Mansc1 (NM_026345) Mouse Tagged ORF Clone

CAT#: MR206544

Mansc1 (Myc-DDK-tagged) - Mouse MANSC domain containing 1 (Mansc1)

Product Images

Circular map for MR206544

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mansc1
• Synonyms: 9130403P13Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206544 representing NM_026345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTTCAGGGGAACCAGCTTGGCTTACTCGTTGCTGGTCATTTCCTTCCTGACACCAAGGTCGTCTG
  CTGGTCAGAACTGCCTCACCAAGAGTCTAGAAGACGTTGTCATTGACATCCAATCGTCGCTTTCAAAAGG
  CATTCGAGGCAATGAGCCCATACACTTGGCAACTCAGGAAGACTGTATCGGTGCCTGCTGTTCAACAAAA
  GACATAGCAGGGGACAAGGCATGTAATTTGATGATCTTTGACACCCGGAAGACAGACAGACAGCCCAACT
  GCTACCTGTTTTTCTGTCCCAGCGAGGATGCCTGTCCGCTGAAGCCAGCCAAGGGCCTTGTGACCTACAG
  GCTCATCAGAGATTTTCCGCTGACCAGCGCTAATTCATCACTCCAACAGTTAACACAAGGAGAGTTTCTC
  TTACTTGACCATTCGTCACCAGGAGCCACCCCTGGGTTTCGTACCCCAGCAGGTTACCCAAAGCCCACCG
  GCCTGTCTTGGAGTGACAGATCTTCTCTGAAGTCCACAGCCCCCCTGCACTTGCGCAAACACATCAAGGC
  TGATGAAACAAGCATGCAGCTCCCTGAAGAAAAAAGCCATTCTCAGAGTTTACAGCTTCCCTCAGAACTA
  AAAATGGCTCATCTGCTCCCTAAAACTGTGCCGACTCCACCTACCACTGTAGCTGTGGCTCCCCTCCGTA
  ACGTCTCTGCCACCCTGAAGCCTGAGCTTCTGTTGACCAGCATTTCAGTGACAGCTAAGACTTTGAAGCA
  GAAGGAGGCCACCACAGCATCACCTGTCACCACCGTGACCTCGAAGCTCCCAGGAGTCCCAGGGTCTACA
  AGTTTTACACCCGTGGTTACACATCAGGCAGCTTTGACTAACACCTTTCAGGCACATACAGACTCCAAAG
  GCATCTTAGAAACAATGCCCTTTCAAGGAGGCTCTACGCTGACTTCAGACCCGAGGCACGGGAAAAGCTC
  AACCTCAGAGTCTTCCATTACAAACAAGACTGCTTCCTGGGAGGACAGGAGGGTCAGTGTAGGCAGCGCA
  TCGCTGAACAAGGGTCCAAAAAGCCAGCATGGCCTTTCATTTGAGAAGTGGCTTCTCATTGGGACCCTCC
  TTTGTGGTGTTTTGTTTCTGGTAATAGGTCTCGTCCTCTTGGGTAGGATGCTGGTTGAAGCCCTCCGTAG
  GAAACGGTATTCAAGACTTGACTACTTGATCAACGGGATCTATGTTGACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206544 representing NM_026345
  Red=Cloning site Green=Tags(s)
  
  MLFRGTSLAYSLLVISFLTPRSSAGQNCLTKSLEDVVIDIQSSLSKGIRGNEPIHLATQEDCIGACCSTK
  DIAGDKACNLMIFDTRKTDRQPNCYLFFCPSEDACPLKPAKGLVTYRLIRDFPLTSANSSLQQLTQGEFL
  LLDHSSPGATPGFRTPAGYPKPTGLSWSDRSSLKSTAPLHLRKHIKADETSMQLPEEKSHSQSLQLPSEL
  KMAHLLPKTVPTPPTTVAVAPLRNVSATLKPELLLTSISVTAKTLKQKEATTASPVTTVTSKLPGVPGST
  SFTPVVTHQAALTNTFQAHTDSKGILETMPFQGGSTLTSDPRHGKSSTSESSITNKTASWEDRRVSVGSA
  SLNKGPKSQHGLSFEKWLLIGTLLCGVLFLVIGLVLLGRMLVEALRRKRYSRLDYLINGIYVDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026345
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026345.4, NP_080621.1
• RefSeq Size: 2405 bp
• RefSeq ORF: 1245 bp
• Locus ID: 67729
• UniProt ID: Q9CR33
• Cytogenetics: 6 G1
• MW: 45.3 kDa