Azi2 (NM_013727) Mouse Tagged ORF Clone

CAT#: MR206364

Azi2 (Myc-DDK-tagged) - Mouse 5-azacytidine induced gene 2 (Azi2), transcript variant 1

Product Images

Circular map for MR206364

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Azi2
• Synonyms: AA410145; AZ2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206364 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACGCTAGTAGAAGATGACATCTGCATTCTGAATCATGAAAAGGCCCACAGGAGAGAGGCAGTGA
  CTCCACTCTCAGCGTACCCTGGAGATGAGTCTGTTGCTTCCCATTTCGCACTGGTCACTGCTTATGAAGA
  CATCAAAAAGAGGCTGAAGGATTCAGAGAAAGAAAACTCCTTCTTAAAGAAAAGAATCAGGGCTTTGGAA
  GAAAGGCTTGTTGGAGCTCGAGCAGATGAAGAAACAAGTTCTGTGGGCCGAGAACAAGTGAATAAGGCCT
  ATCATGCATATCGTGAGGTTTGCATTGATAGAGACAATCTGAAGAACCAATTGGAAAAGATAAATAAAGA
  CAACTCTGAATCTTTGAAAATGTTGAATGAGCAGCTACAGTCTAAAGAAGTAGAACTTCTGCAGCTAAGA
  ACAGAGGTGGAGACTCAGCAGGTGATGAGGAATTTAAACCCACCCTCATCCAGCTGGGAGGTGGAGAAGC
  TAAGCTGTGACTTGAAGATCCATGGTTTGGAGCAAGAGCTGGGACTACTGAGGAAGGAGTGCAGTGACCT
  CAGGACAGAGCTGCAGAAGGCCAGACAGACGGGTCCACCTCAGGAAGACATTCTGCAGGGCAGAGATGTC
  ATCAGGCCGAGCTTGTCGAGAGAAGAGCATGTTCCACACCAGGGTCTTCACCACAGTGATAATATGCAAC
  ATGCATACTGGGAACTGAAGAGGGAGATGTCTAACTTACACCTGGTGACTCAAGTGCAAGCAGAACTACT
  AAGAAAACTGAAAACCTCAGCTGCAGTCAAGAAAGCCTGTACCCCAGTAGGATGTGTTGAAGACCTTGGG
  AGAGACAGCACGAAATTGCACTTGACAAATTTTACTGCAACTTACAAAAGACATCCCTCTTTGTCACCAA
  ATGGCAAAGCTCCTTGTTATGCTCCGTCTTCCCCTTTACCAGGAGATAGAAAGGTTTTCTCAGACAAAGC
  AGTTCTTCAGTCATGGACAGATAACGAGAGATTGGTTCCTAATGACGGTGCAGACTTTCCAGAGCACAGC
  TCCTATGGGAGAAATTCTCTGGAAGATAATTCCTGGGTATTCCCAAGCCCTCCTAAATCAAGTGAGACAG
  CATTTGGTGAAAATAAAAGCAAAATCTTACCTTTATCCAACCTGCCGCCACTGCATTACCTGGATCAACA
  GAATCAGAACTGCCTTTACAAAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206364 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDTLVEDDICILNHEKAHRREAVTPLSAYPGDESVASHFALVTAYEDIKKRLKDSEKENSFLKKRIRALE
  ERLVGARADEETSSVGREQVNKAYHAYREVCIDRDNLKNQLEKINKDNSESLKMLNEQLQSKEVELLQLR
  TEVETQQVMRNLNPPSSSWEVEKLSCDLKIHGLEQELGLLRKECSDLRTELQKARQTGPPQEDILQGRDV
  IRPSLSREEHVPHQGLHHSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTSAAVKKACTPVGCVEDLG
  RDSTKLHLTNFTATYKRHPSLSPNGKAPCYAPSSPLPGDRKVFSDKAVLQSWTDNERLVPNDGADFPEHS
  SYGRNSLEDNSWVFPSPPKSSETAFGENKSKILPLSNLPPLHYLDQQNQNCLYKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013727
• ORF Size: 1218 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013727.4
• RefSeq Size: 4631 bp
• RefSeq ORF: 1218 bp
• Locus ID: 27215
• UniProt ID: Q9QYP6
• Cytogenetics: 9 F3
• MW: 46.1 kDa
• Gene Summary: Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.[UniProtKB/Swiss-Prot Function]