Mak (BC050009) Mouse Tagged ORF Clone

CAT#: MR206262

Mak (Myc-DDK-tagged) - Mouse male germ cell-associated kinase (cDNA clone MGC:25408 IMAGE:4527843)

Product Images

Circular map for MR206262

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mak
• Synonyms: A930010O05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206262 representing BC050009
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGATACACAACCATGAAGCAGCTGGGAGACGGCACGTATGGGAGTGTACTGATGGGCAAGAGCA
  ATGAGTCTGGGGAGCTGGTGGCAATCAAGAGGATGAAGAGAAAGTTCTATTCTTGGGATGAGTGTATGAA
  CTTGCGAGAAGTTAAGTCCCTGAAAAAACTCAATCACGCCAATGTGATTAAACTAAAAGAAGTTATCAGA
  GAGAATGACCATCTTTATTTTGTATTTGAATATATGAAAGAAAACCTCTATCAGCTAATGAAAGACAGAA
  ACAAACTGTTCCCTGAGTCAGTCATCAGAAATATTATGTATCAGATACTACAGGGGCTGGCATTTATCCA
  TAAACACGGCTTTTTTCACAGGGACATGAAACCTGAGAACTTGCTCTGCATGGGCCCAGAGCTGGTGAAG
  ATTGCTGATTTTGGACTTGCAAGAGAATTAAGATCACAGCCACCATATACTGACTATGTGTCTACCAGAT
  GGTACCGGGCTCCTGAAGTCTTACTAAGGTCTTCGGTGTACAGCTCTCCCATTGACGTGTGGGCCGTGGG
  AAGTATAATGGCCGAGTTATATACATTTAGGCCGCTTTTCCCAGGGACAAGTGAAGTTGATGAGATCTTT
  AAAATCTGCCAAGTGTTAGGGACTCCCAAGAAGAGTGACTGGCCGGAAGGATACCAGCTTGCATCCTCCA
  TGAACTTCCGCTTTCCCCAGTGCATTCCCATCAACCTAAAAACTCTCATTCCCAATGCCAGTAGCGAGGC
  CATCCAGCTCATGACAGAGATGCTGAACTGGGATCCAAAGAAGCGACCAACAGCAAGCCAGGCACTGAAA
  CACCCATATTTCCAAGTCGGTCAGGTACTGGGCTCTTCTGCACACCATCTGGATACAAAACAGACTTTAC
  ACAAGCAGCTGCAGCCTCTGGAGCCAAAGCCATCTTCCTCTGAACGGGATCCTAAGCCTTTGCCAAACAT
  ACTTGATCAGCCTGCCGGGCAGCCCCAGCCAAAACAGGGCCACCAACCACTGCAGACCATTCAGCCACCA
  CAGAACACAGTGACTCACCCACCCCCAAAGCAGCAGGGTCACCAGAAACCACCACAAACCATGTTTCCAA
  GTATCATCAAAACCATCCCAGTGGTGAGTAGCCAAGCCGATGCTTTAGATGACACGGACTGGTGCACTCT
  GGGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206262 representing BC050009
  Red=Cloning site Green=Tags(s)
  
  MNRYTTMKQLGDGTYGSVLMGKSNESGELVAIKRMKRKFYSWDECMNLREVKSLKKLNHANVIKLKEVIR
  ENDHLYFVFEYMKENLYQLMKDRNKLFPESVIRNIMYQILQGLAFIHKHGFFHRDMKPENLLCMGPELVK
  IADFGLARELRSQPPYTDYVSTRWYRAPEVLLRSSVYSSPIDVWAVGSIMAELYTFRPLFPGTSEVDEIF
  KICQVLGTPKKSDWPEGYQLASSMNFRFPQCIPINLKTLIPNASSEAIQLMTEMLNWDPKKRPTASQALK
  HPYFQVGQVLGSSAHHLDTKQTLHKQLQPLEPKPSSSERDPKPLPNILDQPAGQPQPKQGHQPLQTIQPP
  QNTVTHPPPKQQGHQKPPQTMFPSIIKTIPVVSSQADALDDTDWCTLGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC050009
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC050009
• RefSeq Size: 6818 bp
• RefSeq ORF: 1199 bp
• Locus ID: 17152
• Cytogenetics: 13 20.25 cM
• MW: 249.9 kDa
• Gene Summary: Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Could have an important function in sensory cells and in spermatogenesis. May participate in signaling pathways used in visual and olfactory sensory transduction. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR (By similarity).[UniProtKB/Swiss-Prot Function]