St8sia5 (NM_153124) Mouse Tagged ORF Clone

CAT#: MR205839

St8sia5 (Myc-DDK-tagged) - Mouse ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant 2

Product Images

Circular map for MR205839

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: St8sia5
• Synonyms: Siat8e; ST8SiaV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205839 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTACGCAGACCCCTCCGCCAACAGAGATTTGTTGGGGAATCGAACTTTGCTCTTTATCTTCATCT
  GCGCCTTTGCCTTGGTGACCTTGCTTCAGCAGATCCTGTACAGCAAGAGCTACATCAAAAGGTACTTCGA
  GTTTTACAAGGAGCCTTTAGAGTTTAACTCCACGAGATGCCTGGAGCTGAGGCAGGAGATCCTGGAGGTG
  AAGGTGCTGTCCATGGTGAAGCAGTCTGAACTCTTCGAAAGGTGGAAGAGTCTCCAGATATGCAAATGGG
  CAATGGACACCGCGGAAGCCAGCCTCTTCAAGTCCACCTTGTCCAGGTGCTGCAATGCCCCCAGCTTTCT
  CTTCACCACCCAGAAGAACACCCCGGTGGAGACGAATCTCAGGTATGAGGTGGAATCCAGCGGCTTATAT
  CACATCGACCAGGAGATCTTCAAAATGTTTCCCAAGGAAATGCCCTACTACCGCTCTCAGTTTAAGAAGT
  GTGCTGTGGTGGGCAATGGCGGCATCTTGAAGAACAGCGGCTGCGGGAAGGAGATCAACAGTGCTGACTT
  TGTCTTCCGGTGCAACCTGCCCCCCATCTCAGGGATATATACCACAGATGCGGGAGAGAAGACAGATGTA
  GTCACCGTGAATCCCAGCATCATAATTGACAGGTTCCACAAGTTGGAGAAGTGGCGGCGGCCTTTCTTCA
  GCGTGCTGCAGAGGTACGAGAACGCCTCAGTGCTGCTGCCAGCGTTCTACAACGTCCGCAACACCCTGGT
  ATCCTTCCGAGTCAAATACATGCTGGACGACTTCCAGTCGCGGCAGCCGGTCTACTTCTTCCACCCGCAG
  TACCTGAGCAGTGTGTCGCGCTACTGGCTCAGCCTGGGTGTGCGCGCGCGGCGTATCAGCACCGGCCTCA
  TCCTGGTCACCGCGGCCCTGGAGCTCTGCGAGGAGGTGCACCTGTTCGGTTTCTGGGCCTTCCCCATGAA
  CCCCTCCGGCTTCTTCATCACGCACCATTACTATGACAACGTCAAGCCCAAGCCGGGTTTCCATGCTATG
  CCCTCGGAGATCTTCACCTTCCTGCGCATGCACAGCCGCGGCATCCTTCGCGTGCACACAGGCACCTGCA
  ATTGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205839 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRYADPSANRDLLGNRTLLFIFICAFALVTLLQQILYSKSYIKRYFEFYKEPLEFNSTRCLELRQEILEV
  KVLSMVKQSELFERWKSLQICKWAMDTAEASLFKSTLSRCCNAPSFLFTTQKNTPVETNLRYEVESSGLY
  HIDQEIFKMFPKEMPYYRSQFKKCAVVGNGGILKNSGCGKEINSADFVFRCNLPPISGIYTTDAGEKTDV
  VTVNPSIIIDRFHKLEKWRRPFFSVLQRYENASVLLPAFYNVRNTLVSFRVKYMLDDFQSRQPVYFFHPQ
  YLSSVSRYWLSLGVRARRISTGLILVTAALELCEEVHLFGFWAFPMNPSGFFITHHYYDNVKPKPGFHAM
  PSEIFTFLRMHSRGILRVHTGTCNCC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153124
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153124.1
• RefSeq Size: 2006 bp
• RefSeq ORF: 1131 bp
• Locus ID: 225742
• Cytogenetics: 18 E3
• MW: 43.8 kDa
• Gene Summary: May be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b and GT3 from GD1a, GT1b, GM1b and GD3 respectively.[UniProtKB/Swiss-Prot Function]