Aspn (NM_025711) Mouse Tagged ORF Clone

CAT#: MR205760

Aspn (Myc-DDK-tagged) - Mouse asporin (Aspn), transcript variant 1

Product Images

Circular map for MR205760

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aspn
• Synonyms: 4631401G09Rik; AA986886; PL; Plap1; SL; Slrr1c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205760 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGTATGTGATGCTACTGCTTTTGGCTGTGTGCTCTGCCAAACCCTTCTTTAGCCCTTCCCACA
  CAGCACTGAAGAATATGATGTTGAAGGATATGGAAGACACAGATGATGACGATAACGATGATGACGACAA
  CTCTCTTTTTCCAACGAAAGAGCCAGTGAACCCTTTTTTCCCTTTCGATTTGTTTCCAACATGTCCATTT
  GGGTGCCAATGTTACTCTCGAGTTGTTCACTGCTCTGATCTAGGTCTGACATCGGTTCCAAACAACATTC
  CATTTGATACTCGAATGGTTGACCTTCAAAATAATAAAATCAAGGAAATTAAAGAAAATGACTTTAAAGG
  ACTCACTTCACTTTATGCTCTGATTCTGAACAACAACAAGCTAACAAAGATTCACCCAAAAACCTTTCTA
  ACCACAAAGAAATTGAGAAGGCTATATTTATCCCACAACCAACTAAGTGAAATTCCACTTAATCTTCCCA
  AATCATTAGCAGAACTCAGAATTCATGATAATAAAGTTAAGAAGATACAAAAGGACACGTTCAAGGGAAT
  GAATGCTTTACATGTTTTGGAAATGAGTGCAAACCCTCTTGAGAACAACGGGATAGAACCAGGGGCATTT
  GAAGGGGTGACAGTATTCCATATCAGGATCGCTGAAGCAAAACTAACCTCAATTCCAAAAGGCCTACCAC
  CAACTTTGCTGGAGCTTCATTTAGATTTTAATAAAATTTCAACGGTGGAACTTGAAGATCTTAAACGGTA
  CAGGGAACTGCAAAGGCTGGGTCTTGGAAACAACAGAATCACAGATATTGAAAATGGAACTTTTGCTAAT
  ATACCACGTGTGAGAGAGATACACTTGGAACACAATAAACTAAAAAAAATCCCTTCAGGATTACAGGAGT
  TGAAATACCTCCAGATAATCTTCCTTCATTATAATTCAATTGCAAAAGTGGGAGTGAATGACTTCTGTCC
  AACAGTGCCAAAGATGAAGAAATCTTTATACAGTGCAATAAGTTTATTCAACAACCCAATGAAGTACTGG
  GAAATACAACCTGCAACATTTCGTTGTGTTCTTGGCAGAATGAGTGTTCAGCTTGGGAATGTTGGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205760 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKEYVMLLLLAVCSAKPFFSPSHTALKNMMLKDMEDTDDDDNDDDDNSLFPTKEPVNPFFPFDLFPTCPF
  GCQCYSRVVHCSDLGLTSVPNNIPFDTRMVDLQNNKIKEIKENDFKGLTSLYALILNNNKLTKIHPKTFL
  TTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHDNKVKKIQKDTFKGMNALHVLEMSANPLENNGIEPGAF
  EGVTVFHIRIAEAKLTSIPKGLPPTLLELHLDFNKISTVELEDLKRYRELQRLGLGNNRITDIENGTFAN
  IPRVREIHLEHNKLKKIPSGLQELKYLQIIFLHYNSIAKVGVNDFCPTVPKMKKSLYSAISLFNNPMKYW
  EIQPATFRCVLGRMSVQLGNVGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025711
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025711.3, NP_079987.2
• RefSeq Size: 2362 bp
• RefSeq ORF: 1122 bp
• Locus ID: 66695
• UniProt ID: Q99MQ4
• Cytogenetics: 13 A5
• MW: 42.6 kDa
• Gene Summary: This gene encodes a member of the small leucine-rich proteoglycan family. The encoded protein is an extracellular matrix protein that modulates the transforming growth factor-beta signaling pathway, regulating cartilage matrix gene expression and cartilage formation. The protein plays a role in the pathology of osteoarthritis. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]