Eif4b (BC007171) Mouse Tagged ORF Clone

CAT#: MR205574

Eif4b (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114)

Product Images

Circular map for MR205574

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eif4b
• Synonyms: 2310046H11Rik; AL024095; C85189; Eif4a2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205574 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGCTATGGGGGCCGGGATCGCTATGATGACCGAGGCAGCAGAGACTATGACCGAGGCTATGACT
  CCAGGATAGGCAGTGGCAGAAGAGCATTTGGAAGTGGGTACCGGAGAGATGATGACTACAGAGGAGGTGG
  GGACCGCTATGAAGACCGCTATGACAGACGGGATGATCGGTCGTGGAGCTCCAGGGATGACTACTCTCGG
  GATGATTATAGGCGTGATGACAGAGGTCCCCCCCAGAGACCCAGACTGAACCTCAAGCCTCGCAGCGCTC
  CTAAGGAGGATGACGCCTCCGCCAGCACCTCCCAGTCCAGCCGGGCAGCCTCCATCTTTGGAGGGGCGAA
  GCCTGTTGACACAGCTGCTAGGGAAAGAGAAGTAGAGGAGCGGCTACAGAAGGAGCAGGAGAAGCTGCAG
  CGTCAGCTGGATGAGCCAAAACTAGACCGCCGGCCCCGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAA
  CTCAGGAAAGAGAACGGTCAAGGACAGGAAGTGAGTCATCGCAGACTGGGGCCTCAGCCACATCTGGCAG
  AAATACACGAAGGAGAGAGAGTGAGAAGTCTCTAGAAAATGAAACCCTCAATAAAGAAGAAGACTGTCAC
  TCTCCAACCTCTAAGCCTCCTAAACCTGACCAGCCTCTAAAGGTAATGCCAGCCCCTCCACCAAAGGAGA
  ATGCGTGGGTGAAGCGAAGCTCTAACCCTCCTGCCCGATCTCAGAGCTCAGACACAGAGCAGCCGTCCCC
  TACAAGTGGTGGAGGGAAAGTAGCTGCAGTCCAGCCCCCTGAGGAAGGACCATCAAGAAAAGATGGAAAT
  AAAGTGGATGTGGTGGGTGCCACACAAGGCCAAGCTGGAAGCTGCAGCCGTGGTCCCGGGGATGGAGGGA
  GCAGAGACCACTGGAAGGACTTGGATAGGAAGGATGGCAAAAAAGATCAAGATTCCAGATCTGCACCTGA
  GCCAAAGAAACCTGAGGAGAACCCAGCCTCCAAGTTCAGCTCTGCAAGCAAGTACGCTGCTCTGTCTGTG
  GATGGCGAGGATGAGGATGAGGGCGACGACTGCACTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205574 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSR
  DDYRRDDRGPPQRPRLNLKPRSAPKEDDASASTSQSSRAASIFGGAKPVDTAAREREVEERLQKEQEKLQ
  RQLDEPKLDRRPRERHPSWRSEETQERERSRTGSESSQTGASATSGRNTRRRESEKSLENETLNKEEDCH
  SPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQPSPTSGGGKVAAVQPPEEGPSRKDGN
  KVDVVGATQGQAGSCSRGPGDGGSRDHWKDLDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSV
  DGEDEDEGDDCTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC007171
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC007171, AAH07171
• RefSeq Size: 3658 bp
• RefSeq ORF: 1091 bp
• Locus ID: 75705
• Cytogenetics: 15 F2
• MW: 40.7 kDa
• Gene Summary: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F (By similarity).[UniProtKB/Swiss-Prot Function]