Cbx8 (NM_013926) Mouse Tagged ORF Clone

CAT#: MR205544

Cbx8 (Myc-DDK-tagged) - Mouse chromobox homolog 8 (Drosophila Pc class) (Cbx8)

Product Images

Circular map for MR205544

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cbx8
• Synonyms: Pc3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205544 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCTCGGCGGTGGGGGAGCGGGTGTTCGCGGCCGAAGCCCTCCTGAAGCGGCGCATTCGCAAAG
  GACGCATGGAATATCTCGTGAAATGGAAGGGCTGGTCGCAGAAGTACAGCACGTGGGAGCCCGAAGAAAA
  TATTCTGGATGCTCGCCTCCTTGCAGCCTTTGAGGAAAGGGAACGGGAGATGGAGCTCTATGGCCCCAAA
  AAGCGAGGACCCAAGCCTAAAACCTTCCTTCTCAAGGCCCAGGCCAAGGCAAAGGCCAAAACCTATGAAT
  TCAGAAGTGACTCTACCAGAGGCATCCGGATCCCTTACCCAGGCCGCTCACCCCAGGATTTGGCGTCTAC
  TTCCAGGGCCCGAGAGGGCCTGCGGAACACGGGGCTACCCCCACCAGGGAGCAGCACCAGTACCTGCAGG
  GCAGACCCACCTCGGGACCGGGACCGAGAGAGGGACAGGGGTACCAGCCGTGTAGACGACAAGCCCAGCT
  CACCGGGGGACAGCTCCAAGAAACGAGGACCGAAACCCAGGAAGGAGCCCCTGGACCCTTCACAGAGACC
  TTTGGGAGAGCCCAGTGCCGGCCTTGGAGAGTACCTGAAAGGCAGGAAGCTGGATGAGACCTCTTCTGGG
  ACAGGAAAGTTCCCAGCTGGCCACAGTGTGATCCAGCTTGCTCGAAGGCAGGACTCAGACCTGGTGCAAT
  ATGGTGTGACCAGTCCTAGCTCAGCAGAGGCCTCGAGCAAGTTGGCTGTGGACACCTTTCCAGCCAGGGT
  AATAAAGCACAGGGCTGCTTTCCTGGAGGCCAAAGGCCAAGGTGCCCTGGACCCTGGTGGTGCCAGGGTT
  CGACATAGTTCAGGCACCCCAGCCTCAGTGGGAAGCCTGTATCGGGACATGGGGGCGCAAGGGGGAAGGC
  CCTCCCTCATCGCCAGGATCCCAGTGGCCAGGATCCTGGGGGACCCAGAGGAAGAGTCCTGGAGCCCCTC
  TCTGACTAACCTGGAGAAAGTGGTCGTTACAGATGTGACCTCAAACTTTTTGACCGTCACCATTAAGGAG
  AGCAACACGGACCAAGGATTCTTTAAGGAAAAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205544 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPK
  KRGPKPKTFLLKAQAKAKAKTYEFRSDSTRGIRIPYPGRSPQDLASTSRAREGLRNTGLPPPGSSTSTCR
  ADPPRDRDRERDRGTSRVDDKPSSPGDSSKKRGPKPRKEPLDPSQRPLGEPSAGLGEYLKGRKLDETSSG
  TGKFPAGHSVIQLARRQDSDLVQYGVTSPSSAEASSKLAVDTFPARVIKHRAAFLEAKGQGALDPGGARV
  RHSSGTPASVGSLYRDMGAQGGRPSLIARIPVARILGDPEEESWSPSLTNLEKVVVTDVTSNFLTVTIKE
  SNTDQGFFKEKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013926
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013926.1, NP_038954.1
• RefSeq Size: 1413 bp
• RefSeq ORF: 1089 bp
• Locus ID: 30951
• UniProt ID: Q9QXV1
• Cytogenetics: 11 E2
• MW: 39.9 kDa
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity).[UniProtKB/Swiss-Prot Function]