Oas1e (NM_145210) Mouse Tagged ORF Clone

CAT#: MR205431

Oas1e (Myc-DDK-tagged) - Mouse 2'-5' oligoadenylate synthetase 1E (Oas1e)

Product Images

Circular map for MR205431

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Oas1e
• Synonyms: C87484; oasl7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205431 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGGGAACTCTTCAGAACCCCAATCTGGAGGCTGGATAACTTCATAGAGGATCAACTCCTTCCTG
  ACACCACCTTCCTTACTGAGCTCAGAGCAGACATCGACTCCATAAGTGCTTTCCTGAAGGAGAGATGCTT
  CCAAGGTGCCACCCACCCTATGAGGGTCGCCAGGGTTGTGATGGGAGGCTCCTATGATGAACACAATGCA
  CTCAAGGGAAGGTCAGAGGCCAACATGGTGGTGTTCTTTAATGATCTCACCAGCTTTGAGGACCAGTTAA
  AGTGGCAGCAAGTGTTCATTGAAGAAATTCGGAAACACCTGCTCCAGTTGCAGCAAGAGAAGCCATGTAA
  ACTCAAGTTTGAGGTGCAGAGCTCAGAGGAGCCCAACACCAGGTCTCTGACCTTCAAGCTGTGCTCCCCA
  GAGCGCCAGCAGGAGGTGGAATTTGATGTGCAGCCAGCCTATGATGCTCTGTATGAAGGGGGATACTGCA
  AGTCCTTTGAATCCATTAACTACAACAAAGTCTACGCCCAACTCATCCATGAGTGCACCACCCTGGAGAA
  GGAGGGCGAGTTCTCCATCTGCTTCACCGACCTTCATCAGAGCTTCCTGAGGTATCGTGCGCCCAAGCTC
  TGGAACCTCATCCGTCTGGTCAAGCACTGGTATCAACTGTGTAAGGAGAAGCTGAGGGGACCGCTGCCTC
  CACAGTATGCCCTGGAGCTGCTCACAGTCTACGTCTGGGAATTTGGGGTCCAAGACAGCTTTGGACTCCA
  TGCAGCCCAGTGCTTCCGAACGGTCTTAGAACTGGTCACCAAGTACAAATGCCTTCTAATCTACTGGACA
  TGGTTTTATGACTTTCGACCTGAGATCTCTGACTACCTGCACGGACAGATCAAAAAAGCCAGGCCTCTGA
  TCCTGGATCCGGCAGACCCAACAAGGAACGTGGCTGGTTCAGACTTACAGGCATGGGACCTGCTGGCAAA
  GGAGGCTCAGACCTGGATACATTCAAATTTTTTTAGGAACTGTGATATGTCCCTTGTGAATGGCTGGGAA
  GTGTCGCCAGAGAAACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205431 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARELFRTPIWRLDNFIEDQLLPDTTFLTELRADIDSISAFLKERCFQGATHPMRVARVVMGGSYDEHNA
  LKGRSEANMVVFFNDLTSFEDQLKWQQVFIEEIRKHLLQLQQEKPCKLKFEVQSSEEPNTRSLTFKLCSP
  ERQQEVEFDVQPAYDALYEGGYCKSFESINYNKVYAQLIHECTTLEKEGEFSICFTDLHQSFLRYRAPKL
  WNLIRLVKHWYQLCKEKLRGPLPPQYALELLTVYVWEFGVQDSFGLHAAQCFRTVLELVTKYKCLLIYWT
  WFYDFRPEISDYLHGQIKKARPLILDPADPTRNVAGSDLQAWDLLAKEAQTWIHSNFFRNCDMSLVNGWE
  VSPEKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145210
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145210.2
• RefSeq Size: 1715 bp
• RefSeq ORF: 1071 bp
• Locus ID: 231699
• Cytogenetics: 5 60.64 cM
• MW: 42 kDa