Kmt5c (NM_146177) Mouse Tagged ORF Clone

CAT#: MR205370

Suv420h2 (Myc-DDK-tagged) - Mouse suppressor of variegation 4-20 homolog 2 (Drosophila) (Suv420h2), transcript variant 1

Product Images

Circular map for MR205370

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kmt5c
• Synonyms: BC024816; Suv4-20h2; Suv420h2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205370 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACCAATGGGGCCAAGATTGTGTCTACCCGTGCTTGGAAGAAGAATGAGAAGCTGGAGCTGCTGG
  TGGGCTGCATTGCAGAGCTGCGTGAAGAGGATGAAGACCTGCTTAGGGCCGGTGAGAACGACTTCAGCAT
  CATGTACTCCACCCGAAAAAGGAGTGCCCAGCTGTGGCTTGGCCCAGCTGCCTTCATCAACCATGACTGC
  AAACCCAACTGCAAGTTTGTGCCCTCAGATGGGAATACTGCCTGTGTGAAGGTGCTACGGGACATTGAGC
  CAGGGGATGAAGTGACTTGCTTCTATGGTGAGGGCTTCTTCGGCGAGAAGAATGAGCACTGTGAATGCTA
  TACCTGTGAGAGGAAAGGCGAGGGAGCTTTCAGACTTCAGCCCAGGGAGCCTGAGCTGCGACCAAAGCCC
  CTAGACAAGTATGAGCTCCGAGAGACAAAGAGGCGTCTGCAGCAGGGCTTGGTCAGCAGCCAGCAGAGCC
  TGATGAGCCGGTGGGCCTGCTCCCACCTGTCCCCACTGCGCCCTGACCCATTCTGTGCTGCCTGCCAGCC
  CTCATGCCTGCTGCCTGCCAGCCCCCACATGGATTACTTGCCCCTTTGGCTCCAGCGGGCGCCTCAACCC
  CAGCCCATAGTACCTCCCAGGAAGCGCCACCGCCGCCGCCGCCCCAGGATCCGCCAGGCTTCATTGCCTC
  CTGTCCTCCGCACTGCCTGTGTCCCCCTGCACCGATGGGGAGGCTGTGGTCCCCACTGCCAGCTACGTGC
  TGAGGCCATGGTGACCCTACACCTGCGCCCCCAGACTCGCTGGACCCCACAACAGGACTGGTACTGGGCC
  CGGCGCTATGGGCTACCTTCTGTGGGGCGTGTGGAACTTACCCGCCTAGCCCCAGCCCTGCCAGCTGCTC
  CTGCCCCTGCTGGGAACCCAGGTCCTGTCCCCACTCCTGATTTCATCCCTAAGCAGGCCCTTGCCTTTGC
  TCCGTTCTGCCCACCTAAGCGCCTCCGGCTAGTGGTCAGTCATGGTTCTATTGATCTGGACATCAATAGT
  GGTGAGCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205370 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METNGAKIVSTRAWKKNEKLELLVGCIAELREEDEDLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDC
  KPNCKFVPSDGNTACVKVLRDIEPGDEVTCFYGEGFFGEKNEHCECYTCERKGEGAFRLQPREPELRPKP
  LDKYELRETKRRLQQGLVSSQQSLMSRWACSHLSPLRPDPFCAACQPSCLLPASPHMDYLPLWLQRAPQP
  QPIVPPRKRHRRRRPRIRQASLPPVLRTACVPLHRWGGCGPHCQLRAEAMVTLHLRPQTRWTPQQDWYWA
  RRYGLPSVGRVELTRLAPALPAAPAPAGNPGPVPTPDFIPKQALAFAPFCPPKRLRLVVSHGSIDLDINS
  GEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146177
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146177.1, NP_666289.1
• RefSeq Size: 2240 bp
• RefSeq ORF: 1407 bp
• Locus ID: 232811
• UniProt ID: Q6Q783
• Cytogenetics: 7 A1
• MW: 39.9 kDa
• Gene Summary: Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2).[UniProtKB/Swiss-Prot Function]