Mr1 (NM_008209) Mouse Tagged ORF Clone

CAT#: MR205125

Mr1 (Myc-DDK-tagged) - Mouse major histocompatibility complex, class I-related (Mr1)

Product Images

Circular map for MR205125

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mr1
• Synonyms: H2ls
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205125 representing NM_008209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTCCTGTTACCTCTGCTCGCTGTATTCTTGGTGAAGCGAAGCCATACTCGGACCCACTCGCTGA
  GATATTTTCGTCTAGCTGTTTCCGATCCTGGTCCCGTCGTCCCTGAATTTATCTCTGTTGGGTATGTGGA
  CTCACACCCTATCACTACATACGACAGTGTCACTCGACAGAAGGAGCCGAAAGCTCCATGGATGGCAGAG
  AACCTGGCACCTGATCACTGGGAGAGGTACACTCAGCTGCTAAGGGGCTGGCAGCAGACATTCAAGGCGG
  AGCTGAGGCACCTACAGAGGCACTACAACCACTCAGGGCTTCACACCTACCAGAGAATGATTGGCTGTGA
  GTTGCTAGAAGATGGCAGCACCACAGGGTTTCTCCAGTATGCATATGATGGACAAGATTTCATCATCTTC
  AATAAAGACACCCTCTCCTGGTTGGCCATGGATTATGTGGCTCACATCACCAAGCAAGCATGGGAGGCCA
  ACCTGCATGAGTTGCAATACCAAAAGAACTGGCTGGAAGAAGAATGCATTGCCTGGCTAAAGAGGTTCTT
  GGAATATGGAAGAGATACCCTAGAAAGAACAGAGCATCCAGTGGTAAGAACAACTCGAAAGGAAACTTTT
  CCAGGGATTACAACTTTCTTCTGCAGAGCTCATGGCTTCTACCCACCAGAAATTTCCATGACATGGATGA
  AAAATGGGGAAGAAATTGCCCAAGAAGTGGATTACGGAGGGGTACTTCCCAGCGGGGATGGAACCTATCA
  AACGTGGCTGTCAGTTAATCTGGATCCTCAGAGCAATGATGTTTATTCTTGTCATGTGGAGCACTGTGGT
  CGCCAAATGGTTCTGGAGGCCCCTCGGGAATCAGGGGACATCCTTCGAGTGAGCACGATCTCTGGAACCA
  CAATTCTCATCATCGCCCTGGCTGGAGTTGGTGTTCTGATCTGGAGAAGGTCACAAGAACTAAAAGAAGT
  CATGTACCAACCCACCCAAGTGAATGAGGGAAGCTCTCCCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205125 representing NM_008209
  Red=Cloning site Green=Tags(s)
  
  MMLLLPLLAVFLVKRSHTRTHSLRYFRLAVSDPGPVVPEFISVGYVDSHPITTYDSVTRQKEPKAPWMAE
  NLAPDHWERYTQLLRGWQQTFKAELRHLQRHYNHSGLHTYQRMIGCELLEDGSTTGFLQYAYDGQDFIIF
  NKDTLSWLAMDYVAHITKQAWEANLHELQYQKNWLEEECIAWLKRFLEYGRDTLERTEHPVVRTTRKETF
  PGITTFFCRAHGFYPPEISMTWMKNGEEIAQEVDYGGVLPSGDGTYQTWLSVNLDPQSNDVYSCHVEHCG
  RQMVLEAPRESGDILRVSTISGTTILIIALAGVGVLIWRRSQELKEVMYQPTQVNEGSSPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008209
• ORF Size: 1023 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008209.5
• RefSeq Size: 2509 bp
• RefSeq ORF: 1026 bp
• Locus ID: 15064
• UniProt ID: Q8HWB0
• Cytogenetics: 1 G3
• MW: 39.8 kDa
• Gene Summary: Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites (By similarity). Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent.[UniProtKB/Swiss-Prot Function]