Zbtb25 (NM_028356) Mouse Tagged ORF Clone

CAT#: MR204761

Zbtb25 (Myc-DDK-tagged) - Mouse zinc finger and BTB domain containing 25 (Zbtb25), transcript variant 2

Product Images

Circular map for MR204761

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zbtb25
• Synonyms: 2810462M08Rik; 2900064P18Rik; AI842128; Kup; Zfp-50; Zfp50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204761 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCAAGTGTTCTCACCAGAGACTGTGCAGACATCTAATTTGTATGGTATTCAGATTTCAACAACCC
  AGAAAACAGCTGTCAAACAAGGGCTGGAGATCAAAGAAACTCCTCCCAGTAGCAGTGGAAACAGAGCTGC
  TGTCCCAGGTGACCACCCCCAGCTGCAGCTCTCTCTGGCAATTGGGCTAGATGATGGCAGCGGTGCGGAG
  CAGCAGAGGACCCATCCTGCTGCCCAGGCCGTGGAGGAGCACCAGAAGCCCCCAGTGTCCATCAAGCAGG
  AGAGATGTGATCCAGAGTCTGTGATCTCCCAAAGCCACTCCTCATCCTCATCAGAGGTGACTGGCCCCTC
  TTTTACAGAAAACAGTATCAAAGTCCACTTATGCCATTACTGTGGGGAGCGCTTTGAATCCCGAAGTAAC
  CTGAGACAACACCTCCATACCCACGTGTCCAGATCTCTCCCGTTCGGTGTCCCTGCTTCCATTTTGGAAA
  GTAATGACCTTGGTGAAGTGCATCCACTTAGTGACAGCAGTGAGGCTCTTGAATGCCGGAGGCTCAGCTC
  CTTCATTGTCAAGGAGAATGAGCAGCAGCAGCCATCACCTGAGCACTCGAGCCGAGGCACCTCAGAGCCT
  TTGCAGATTAGTCAAGTATCTTTGATCTCCAAAGACACAGAGCCAGTAGAATTAAACTGTAATTTTTCTT
  TTTCAAGGAAAAGAAAAATCAGTTGTACCATCTGTGGTCATAAATTTCTTCGAAAGAGCCAGTTACTGGA
  ACACATGTATACACACAAAGGTAAATCCTACAGATATAACCGTTGCCAAAGGTTTGGAAACTCATTGGCC
  CAGAGGTTTCAGCAGTATTGTGACAGCTGGCCCGATGTCCCCTTAAAAAGCTCTCGCTTGTCACAAGAAC
  ATTTAGACTTGCCTTGTGCCTTAGAGTCAGAGCTCACACAAGCGGGTGTGGACACCATCCTCGTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204761 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNQVFSPETVQTSNLYGIQISTTQKTAVKQGLEIKETPPSSSGNRAAVPGDHPQLQLSLAIGLDDGSGAE
  QQRTHPAAQAVEEHQKPPVSIKQERCDPESVISQSHSSSSSEVTGPSFTENSIKVHLCHYCGERFESRSN
  LRQHLHTHVSRSLPFGVPASILESNDLGEVHPLSDSSEALECRRLSSFIVKENEQQQPSPEHSSRGTSEP
  LQISQVSLISKDTEPVELNCNFSFSRKRKISCTICGHKFLRKSQLLEHMYTHKGKSYRYNRCQRFGNSLA
  QRFQQYCDSWPDVPLKSSRLSQEHLDLPCALESELTQAGVDTILVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028356
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028356.1, NP_082632.1
• RefSeq Size: 1675 bp
• RefSeq ORF: 1320 bp
• Locus ID: 109929
• Cytogenetics: 12 33.73 cM
• MW: 36.3 kDa