Bub3 (NM_009774) Mouse Tagged ORF Clone

CAT#: MR204752

Bub3 (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) (Bub3)

Product Images

Circular map for MR204752

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bub3
• Synonyms: AU019800; AU021329; AU043350; AW146323; C78067
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204752 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGGTTCGAACGAATTCAAGCTGAACCAGCCACCCGAGGATGGCATCTCCTCGGTTAAGTTCAGCC
  CCAACACATCCCAGTTCCTCCTGGTCTCCTCCTGGGACACGTCTGTGCGCCTCTACGATGTGCCCGCCAA
  TTCCATGAGGCTCAAGTACCAGCACACTGGTGCTGTCCTGGACTGCGCCTTCTACGATCCAACGCATGCC
  TGGAGTGGTGGATTAGATCATCAACTGAAAATGCACGATTTGAACACTGATCAAGAAAATCTTGTTGGAA
  CTCACGATGCCCCTATCAGATGTGTTGAATACTGTCCAGAAGTGAACGTGATGGTAACTGGAAGTTGGGA
  TCAGACAGTTAAACTGTGGGACCCCAGAACTCCGTGTAATGCTGGGACTTTCTCCCAGCCGGAAAAGGTC
  TACACCCTGTCAGTGTCTGGGGACAGGCTGATTGTGGGCACGGCGGGCCGCCGAGTGCTGGTGTGGGACT
  TGCGGAACATGGGCTATGTGCAGCAGCGGAGGGAGTCCAGCCTGAAGTACCAGACTCGCTGCATCCGAGC
  CTTCCCGAACAAGCAGGGTTATGTGTTGAGCTCCATCGAAGGCCGAGTGGCTGTGGAATACTTGGACCCG
  AGCCCTGAGGTGCAGAAGAAGAAGTACGCCTTCAAGTGCCACAGGCTAAAGGAGAACAACATTGAGCAGA
  TTTACCCAGTCAACGCCATCTCCTTTCACAACATCCACAACACGTTTGCCACAGGTGGTTCGGATGGATT
  CGTCAATATTTGGGACCCATTTAACAAGAAGCGCCTGTGCCAGTTCCATCGGTACCCCACCAGCATCGCT
  TCCCTTGCCTTCAGTAATGACGGAACTACGCTTGCAATAGCATCGTCATATATGTATGAGATGGATGACA
  CCGAGCATCCTGAAGATGGTATCTTCATTCGCCAAGTGACAGACGCAGAAACAAAGCCCAAGTCCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204752 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHA
  WSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKV
  YTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDP
  SPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIA
  SLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009774
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009774.1, NM_009774.2, NM_009774.3, NM_009774.4, NP_033904.2
• RefSeq Size: 2055 bp
• RefSeq ORF: 981 bp
• Locus ID: 12237
• UniProt ID: Q9WVA3
• Cytogenetics: 7 74.36 cM
• MW: 37 kDa
• Gene Summary: This gene encodes a component of the mitotic spindle assembly complex. The encoded protein is involved in the regulation of chromosome segregation during oocyte meiosis. Disruption of this gene results in the accumulation of mitotic errors and is lethal in the embryonic stage. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]