Nudt22 (NM_026675) Mouse Tagged ORF Clone

CAT#: MR204358

Nudt22 (Myc-DDK-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 22 (Nudt22)

Product Images

Circular map for MR204358

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nudt22
• Synonyms: 0610006K04Rik; AI851793; AW545547
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204358 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCGAGGTGTCCCTGCTGCTGCTGTGCCCTCTTGGGGGACTTTCCCAGGAGCAGGTAGCAGTGG
  AGCTGAGCCCGGCTCATGACCGTCGTCCATTGCCTGGAGGAGACAAGGCCATCACTGCCATCTGGGAGAC
  TCGGCAACAGGCCCAACCTTGGATCTTTGATGCTCCCAAGTTCCGCCTTCACTCAGCCACACTGGTGTCC
  AGCTCGCCTGAGCCACAGCTGCTCCTGCACTTGGGTCTAACTTCCTACCGGGACTTCCTGGGCACCAACT
  GGTCCAGCTCAGCCTCCTGGCTGCGACAGCAGGGCGCTGCAGATTGGGGTGACAAGCAAGCCTATCTGGC
  AGACCCACTGGGGGTGGGTGCCGCACTGGTCACAGCTGACGACTTCCTTGTCTTCCTGCGCCGCTCTCAG
  CAGGTAGCGGAAGCTCCCGGACTGGTGGATGTACCCGGTGGGCACCCTGAACCTCAGGCCCTGTGCTCTG
  GTGGCATCCCCCGGCACAAGGACCTCCCCGGGGAGCTGGTGGTACGAGAACTCTTCTCCAGTGTCCTACA
  GGAGATCTGTGATGAGGTGAACCTGCCACTGCACACCTTGAGCCAGCCACTGTTGTTGGGAATCGCTTGC
  AATGAGACCAGCGCGGGCAGAGCCAGTGCAGAGTTCTATGTCCAGTGCAGCTTGACTTCAGAGGAGGTCA
  GGAGTTATTACCTGAGTGGGGGACCTGAGGCCCACGAGTCTACAGGAATCATCTTTGTGGAGACACAGAG
  GGTACAGAGACTGCAGGAGACGGAGATGTGGGCACAACTCTGCCCCTCGGCCAAAGGCGCCATCCTCCTC
  TACAATCGCCATCCACCTCTACAGTCAGGTGTTGGGAAGTCCCACCTGAGTCACCCTAGCGCCCCAGCCC
  TATCACTGCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204358 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPEVSLLLLCPLGGLSQEQVAVELSPAHDRRPLPGGDKAITAIWETRQQAQPWIFDAPKFRLHSATLVS
  SSPEPQLLLHLGLTSYRDFLGTNWSSSASWLRQQGAADWGDKQAYLADPLGVGAALVTADDFLVFLRRSQ
  QVAEAPGLVDVPGGHPEPQALCSGGIPRHKDLPGELVVRELFSSVLQEICDEVNLPLHTLSQPLLLGIAC
  NETSAGRASAEFYVQCSLTSEEVRSYYLSGGPEAHESTGIIFVETQRVQRLQETEMWAQLCPSAKGAILL
  YNRHPPLQSGVGKSHLSHPSAPALSLQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026675
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026675.1, NM_026675.2, NP_080951.1
• RefSeq Size: 999 bp
• RefSeq ORF: 927 bp
• Locus ID: 68323
• UniProt ID: Q9DD16
• Cytogenetics: 19 A
• MW: 33.4 kDa
• Gene Summary: Hydrolyzes UDP-glucose to glucose 1-phosphate and UMP and UDP-galactose to galactose 1-phosphate and UMP. Preferred substrate is UDP-glucose.[UniProtKB/Swiss-Prot Function]