Meox2 (NM_008584) Mouse Tagged ORF Clone

CAT#: MR204241

Meox2 (Myc-DDK-tagged) - Mouse mesenchyme homeobox 2 (Meox2)

Product Images

Circular map for MR204241

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Meox2
• Synonyms: AI528662; Gax; Mox-2; Mox2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204241 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACACCCCCTCTTTGGCTGCCTGCGCAGCCCCCACGCCACAGCGCAAGGCTTGCACCCCTTCTCCC
  AGTCTTCTCTGGCCCTCCATGGAAGATCTGACCACATGTCCTACCCCGAACTCTCCACATCTTCCTCGTC
  TTGCATAATCGCGGGATACCCCAACGAGGAGGGCATGTTTGCCAGCCAGCATCACAGGGGGCACCACCAC
  CACCACCACCATCACCATCACCACCACCAGCAGCAGCAGCACCAGGCTCTGCAAAGCAACTGGCACCTCC
  CGCAGATGTCCTCTCCGCCAAGCGCGGCCCGGCACAGCCTGTGCCTGCAGCCTGATTCGGGAGGGCCCCC
  GGAGCTGGGAAGCAGCCCTCCGGTCCTGTGTTCCAACTCATCTAGCCTGGGCTCCAGCACCCCTACCGGG
  GCCGCGTGCGCACCAGGGGATTATGGCCGTCAAGCGCTGTCACCCGCAGACGTAGAGAAGAGAAGTGGCA
  GCAAAAGGAAAAGCGACAGCTCAGATTCCCAGGAAGGAAACTACAAGTCAGAAGTGAACAGCAAACCTAG
  GAAGGAAAGAACAGCTTTTACCAAAGAGCAAATCAGAGAACTAGAGGCAGAATTTGCCCATCATAATTAT
  CTGACCAGACTGAGAAGATATGAGATAGCAGTGAATCTAGACCTCACTGAAAGACAGGTGAAAGTGTGGT
  TCCAGAACAGGAGAATGAAGTGGAAGCGGGTCAAAGGGGGACAGCAAGGAGCTGCTGCCCGAGAAAAGGA
  ACTGGTGAATGTGAAAAAGGGAACGCTTCTTCCGTCAGAGCTGTCAGGAATCGGTGCAGCCACCCTCCAG
  CAGACAGGGGACTCACTAGCAAACGACGACAGCCGCGATAGTGACCACAGCTCTGAGCACGCACACTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204241 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEHPLFGCLRSPHATAQGLHPFSQSSLALHGRSDHMSYPELSTSSSSCIIAGYPNEEGMFASQHHRGHHH
  HHHHHHHHHQQQQHQALQSNWHLPQMSSPPSAARHSLCLQPDSGGPPELGSSPPVLCSNSSSLGSSTPTG
  AACAPGDYGRQALSPADVEKRSGSKRKSDSSDSQEGNYKSEVNSKPRKERTAFTKEQIRELEAEFAHHNY
  LTRLRRYEIAVNLDLTERQVKVWFQNRRMKWKRVKGGQQGAAAREKELVNVKKGTLLPSELSGIGAATLQ
  QTGDSLANDDSRDSDHSSEHAHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008584
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008584.1, NM_008584.2, NM_008584.3, NP_032610.1
• RefSeq Size: 2348 bp
• RefSeq ORF: 912 bp
• Locus ID: 17286
• UniProt ID: P32443
• Cytogenetics: 12 16.84 cM
• MW: 33.5 kDa
• Gene Summary: Mesodermal transcription factor that plays a key role in somitogenesis and is required for sclerotome development. Activates expression of CDKN1A and CDKN2A in endothelial cells, acting as a regulator of vascular cell proliferation. While it activates CDKN1A in a DNA-dependent manner, it activates CDKN2A in a DNA-independent manner. May have a regulatory role when quiescent vascular smooth muscle cells reenter the cell cycle.[UniProtKB/Swiss-Prot Function]