Rnf144a (NM_080563) Mouse Tagged ORF Clone

CAT#: MR204022

Rnf144a (Myc-DDK-tagged) - Mouse ring finger protein 144A (Rnf144a), transcript variant 2

Product Images

Circular map for MR204022

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnf144a
• Synonyms: Rnf144; Ubce7ip4; UIP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204022 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACAGCAAGGTACCGGCCCACCTGGGACCTGGCCCTCGACCCACTGGTGTCCTGCAAGCTGTGCC
  TCGGGGAATACCCAGCGGAGCAGATGACAACCATCGCCCAATGCCAGTGCATCTTCTGTACCCTGTGCCT
  GAAACAGTATGTTGAGCTCTTGATCAAAGAAGGGCTAGAAACTGCAATTAGCTGCCCGGATGCTGCCTGT
  CCCAAACAGGGCCACCTTCAGGAGAATGAGATTGAGTGTATGGTTGCCGCAGAAATAATGCAGAGATATA
  AAAAGCTACAATTTGAAAGAGAGGTGCTCTTTGACCCTTGTCGGACGTGGTGCCCAGCATCCACCTGCCA
  GGCCGTGTGCCAACTCCAGGACATAGGGCTGCAGACCCCGCAGCTGGTACAGTGCAAAGCCTGTGACATG
  GAATTCTGCTCTGCTTGCAAAGCCCGCTGGCACCCTGGCCAAGGCTGCCCAGAGACCATGCCCATCACAT
  TCCTTCCCGGAGAGACCAGCTCTGCTTTCAAGATGGAAGAGGGCGACGCACCCATCAAACGCTGCCCCAA
  GTGCAGGGTGTACATCGAGCGGGATGAGGGCTGTGCACAGATGATGTGTAAGAACTGCAAGCATGCCTTC
  TGCTGGTACTGCCTGGAGTCTCTGGATGATGATTTCCTTCTGATACATTATGACAAGGGGCCCTGCCGCA
  ACAAGCTGGGCCACTCCCGAGCATCAGTGATCTGGCATCGGACACAGGTGGTGGGAATTTTTGCTGGATT
  TGGGCTCCTGCTCTTAGTGGCCTCCCCTTTCCTGCTCCTGGCCACACCCTTCGTGCTTTGCTGCAAATGC
  AAGTGCAGTAAAGGTGACGACGACCCACTGCCCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204022 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTARYRPTWDLALDPLVSCKLCLGEYPAEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDAAC
  PKQGHLQENEIECMVAAEIMQRYKKLQFEREVLFDPCRTWCPASTCQAVCQLQDIGLQTPQLVQCKACDM
  EFCSACKARWHPGQGCPETMPITFLPGETSSAFKMEEGDAPIKRCPKCRVYIERDEGCAQMMCKNCKHAF
  CWYCLESLDDDFLLIHYDKGPCRNKLGHSRASVIWHRTQVVGIFAGFGLLLLVASPFLLLATPFVLCCKC
  KCSKGDDDPLPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080563
• ORF Size: 879 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080563.4, NP_542130.1
• RefSeq Size: 5115 bp
• RefSeq ORF: 879 bp
• Locus ID: 108089
• UniProt ID: Q925F3
• Cytogenetics: 12 A2
• MW: 32.8 kDa
• Gene Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC.[UniProtKB/Swiss-Prot Function]